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Retargeting of macroH2A following mitosis to cytogenetic-scale heterochromatic domains
The heritability of chromatin states through cell division is a potential contributor to the epigenetic maintenance of cellular memory of prior states. The macroH2A histone variant has properties of a regulator of epigenetic cell memory, including roles controlling gene silencing and cell differenti...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6548134/ https://www.ncbi.nlm.nih.gov/pubmed/31110057 http://dx.doi.org/10.1083/jcb.201811109 |
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author | Sato, Hanae Wu, Bin Delahaye, Fabien Singer, Robert H. Greally, John M. |
author_facet | Sato, Hanae Wu, Bin Delahaye, Fabien Singer, Robert H. Greally, John M. |
author_sort | Sato, Hanae |
collection | PubMed |
description | The heritability of chromatin states through cell division is a potential contributor to the epigenetic maintenance of cellular memory of prior states. The macroH2A histone variant has properties of a regulator of epigenetic cell memory, including roles controlling gene silencing and cell differentiation. Its mechanisms of regional genomic targeting and maintenance through cell division are unknown. Here, we combined in vivo imaging with biochemical and genomic approaches to show that human macroH2A is incorporated into chromatin in the G1 phase of the cell cycle following DNA replication. The newly incorporated macroH2A retargets the same large heterochromatic domains where macroH2A was already enriched in the previous cell cycle. It remains heterotypic, targeting individual nucleosomes that do not already contain a macroH2A molecule. The pattern observed resembles that of a new deposition of centromeric histone variants during the cell cycle, indicating mechanistic similarities for macrodomain-scale regulation of epigenetic properties of the cell. |
format | Online Article Text |
id | pubmed-6548134 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-65481342019-12-28 Retargeting of macroH2A following mitosis to cytogenetic-scale heterochromatic domains Sato, Hanae Wu, Bin Delahaye, Fabien Singer, Robert H. Greally, John M. J Cell Biol Research Articles The heritability of chromatin states through cell division is a potential contributor to the epigenetic maintenance of cellular memory of prior states. The macroH2A histone variant has properties of a regulator of epigenetic cell memory, including roles controlling gene silencing and cell differentiation. Its mechanisms of regional genomic targeting and maintenance through cell division are unknown. Here, we combined in vivo imaging with biochemical and genomic approaches to show that human macroH2A is incorporated into chromatin in the G1 phase of the cell cycle following DNA replication. The newly incorporated macroH2A retargets the same large heterochromatic domains where macroH2A was already enriched in the previous cell cycle. It remains heterotypic, targeting individual nucleosomes that do not already contain a macroH2A molecule. The pattern observed resembles that of a new deposition of centromeric histone variants during the cell cycle, indicating mechanistic similarities for macrodomain-scale regulation of epigenetic properties of the cell. Rockefeller University Press 2019-06-28 2019-05-20 /pmc/articles/PMC6548134/ /pubmed/31110057 http://dx.doi.org/10.1083/jcb.201811109 Text en © 2019 Sato et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Sato, Hanae Wu, Bin Delahaye, Fabien Singer, Robert H. Greally, John M. Retargeting of macroH2A following mitosis to cytogenetic-scale heterochromatic domains |
title | Retargeting of macroH2A following mitosis to cytogenetic-scale heterochromatic domains |
title_full | Retargeting of macroH2A following mitosis to cytogenetic-scale heterochromatic domains |
title_fullStr | Retargeting of macroH2A following mitosis to cytogenetic-scale heterochromatic domains |
title_full_unstemmed | Retargeting of macroH2A following mitosis to cytogenetic-scale heterochromatic domains |
title_short | Retargeting of macroH2A following mitosis to cytogenetic-scale heterochromatic domains |
title_sort | retargeting of macroh2a following mitosis to cytogenetic-scale heterochromatic domains |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6548134/ https://www.ncbi.nlm.nih.gov/pubmed/31110057 http://dx.doi.org/10.1083/jcb.201811109 |
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