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Species specific differences in use of ANP32 proteins by influenza A virus
Influenza A viruses (IAV) are subject to species barriers that prevent frequent zoonotic transmission and pandemics. One of these barriers is the poor activity of avian IAV polymerases in human cells. Differences between avian and mammalian ANP32 proteins underlie this host range barrier. Human ANP3...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6548507/ https://www.ncbi.nlm.nih.gov/pubmed/31159925 http://dx.doi.org/10.7554/eLife.45066 |
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author | Long, Jason S Idoko-Akoh, Alewo Mistry, Bhakti Goldhill, Daniel Staller, Ecco Schreyer, Jocelyn Ross, Craig Goodbourn, Steve Shelton, Holly Skinner, Michael A Sang, Helen McGrew, Michael J Barclay, Wendy |
author_facet | Long, Jason S Idoko-Akoh, Alewo Mistry, Bhakti Goldhill, Daniel Staller, Ecco Schreyer, Jocelyn Ross, Craig Goodbourn, Steve Shelton, Holly Skinner, Michael A Sang, Helen McGrew, Michael J Barclay, Wendy |
author_sort | Long, Jason S |
collection | PubMed |
description | Influenza A viruses (IAV) are subject to species barriers that prevent frequent zoonotic transmission and pandemics. One of these barriers is the poor activity of avian IAV polymerases in human cells. Differences between avian and mammalian ANP32 proteins underlie this host range barrier. Human ANP32A and ANP32B homologues both support function of human-adapted influenza polymerase but do not support efficient activity of avian IAV polymerase which requires avian ANP32A. We show here that the gene currently designated as avian ANP32B is evolutionarily distinct from mammalian ANP32B, and that chicken ANP32B does not support IAV polymerase activity even of human-adapted viruses. Consequently, IAV relies solely on chicken ANP32A to support its replication in chicken cells. Amino acids 129I and 130N, accounted for the inactivity of chicken ANP32B. Transfer of these residues to chicken ANP32A abolished support of IAV polymerase. Understanding ANP32 function will help develop antiviral strategies and aid the design of influenza virus resilient genome edited chickens. |
format | Online Article Text |
id | pubmed-6548507 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-65485072019-06-12 Species specific differences in use of ANP32 proteins by influenza A virus Long, Jason S Idoko-Akoh, Alewo Mistry, Bhakti Goldhill, Daniel Staller, Ecco Schreyer, Jocelyn Ross, Craig Goodbourn, Steve Shelton, Holly Skinner, Michael A Sang, Helen McGrew, Michael J Barclay, Wendy eLife Microbiology and Infectious Disease Influenza A viruses (IAV) are subject to species barriers that prevent frequent zoonotic transmission and pandemics. One of these barriers is the poor activity of avian IAV polymerases in human cells. Differences between avian and mammalian ANP32 proteins underlie this host range barrier. Human ANP32A and ANP32B homologues both support function of human-adapted influenza polymerase but do not support efficient activity of avian IAV polymerase which requires avian ANP32A. We show here that the gene currently designated as avian ANP32B is evolutionarily distinct from mammalian ANP32B, and that chicken ANP32B does not support IAV polymerase activity even of human-adapted viruses. Consequently, IAV relies solely on chicken ANP32A to support its replication in chicken cells. Amino acids 129I and 130N, accounted for the inactivity of chicken ANP32B. Transfer of these residues to chicken ANP32A abolished support of IAV polymerase. Understanding ANP32 function will help develop antiviral strategies and aid the design of influenza virus resilient genome edited chickens. eLife Sciences Publications, Ltd 2019-06-04 /pmc/articles/PMC6548507/ /pubmed/31159925 http://dx.doi.org/10.7554/eLife.45066 Text en © 2019, Long et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Microbiology and Infectious Disease Long, Jason S Idoko-Akoh, Alewo Mistry, Bhakti Goldhill, Daniel Staller, Ecco Schreyer, Jocelyn Ross, Craig Goodbourn, Steve Shelton, Holly Skinner, Michael A Sang, Helen McGrew, Michael J Barclay, Wendy Species specific differences in use of ANP32 proteins by influenza A virus |
title | Species specific differences in use of ANP32 proteins by influenza A virus |
title_full | Species specific differences in use of ANP32 proteins by influenza A virus |
title_fullStr | Species specific differences in use of ANP32 proteins by influenza A virus |
title_full_unstemmed | Species specific differences in use of ANP32 proteins by influenza A virus |
title_short | Species specific differences in use of ANP32 proteins by influenza A virus |
title_sort | species specific differences in use of anp32 proteins by influenza a virus |
topic | Microbiology and Infectious Disease |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6548507/ https://www.ncbi.nlm.nih.gov/pubmed/31159925 http://dx.doi.org/10.7554/eLife.45066 |
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