Cargando…

Identification of reference genes for quantitative PCR during C3H10T1/2 chondrogenic differentiation

C3H10T1/2, a mouse mesenchymal stem cell line, is a well-known in vitro model of chondrogenesis that can be easily employed to recapitulate some of the mechanisms intervening in this process. Moreover, these cells can be used to validate the effect of candidate molecules identified by high throughpu...

Descripción completa

Detalles Bibliográficos
Autores principales:	Cappato, Serena, Giacopelli, Francesca, Tonachini, Laura, Ravazzolo, Roberto, Bocciardi, Renata
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Netherlands 2019
Materias:	Methods Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6548758/ https://www.ncbi.nlm.nih.gov/pubmed/30847849 http://dx.doi.org/10.1007/s11033-019-04713-x

Ejemplares similares

Identification and characterization of regulatory elements in the promoter of ACVR1, the gene mutated in Fibrodysplasia Ossificans Progressiva
por: Giacopelli, Francesca, et al.
Publicado: (2013)

The Horizon of a Therapy for Rare Genetic Diseases: A “Druggable” Future for Fibrodysplasia Ossificans Progressiva
por: Cappato, Serena, et al.
Publicado: (2018)

The Role of the 3′UTR Region in the Regulation of the ACVR1/Alk-2 Gene Expression
por: Mura, Marzia, et al.
Publicado: (2012)

High-throughput screening for modulators of ACVR1 transcription: discovery of potential therapeutics for fibrodysplasia ossificans progressiva
por: Cappato, Serena, et al.
Publicado: (2016)

Correction: High-throughput screening for modulators of ACVR1 transcription: discovery of potential therapeutics for fibrodysplasia ossificans progressiva
por: Cappato, Serena, et al.
Publicado: (2016)

Label-free, atomic force microscopy-based mapping of DNA intrinsic curvature for the nanoscale comparative analysis of bent duplexes
por: Buzio, Renato, et al.
Publicado: (2012)

Is There a Biological Basis for Treatment of Fibrodysplasia Ossificans Progressiva with Rosiglitazone? Potential Benefits and Undesired Effects
por: Bocciardi, Renata, et al.
Publicado: (2010)

Genomic Context and Mechanisms of the ACVR1 Mutation in Fibrodysplasia Ossificans Progressiva
por: Ravazzolo, Roberto, et al.
Publicado: (2021)

Identification and Validation of Quantitative PCR Reference Genes Suitable for Normalizing Expression in Normal and Dystrophic Cell Culture Models of Myogenesis
por: Hildyard, John C.W., et al.
Publicado: (2014)

Genetic and Acquired Heterotopic Ossification: A Translational Tale of Mice and Men
por: Cappato, Serena, et al.
Publicado: (2020)

A case of Fibrodysplasia Ossificans Progressiva associated with a novel variant of the ACVR1 gene
por: Cappato, Serena, et al.
Publicado: (2021)

Development of a quantitative PCR method to differentiate between viable and nonviable bacteria in environmental water samples
por: Gedalanga, Phillip B., et al.
Publicado: (2009)

Symmetric curvature descriptors for label-free analysis of DNA
por: Buzio, Renato, et al.
Publicado: (2014)

Nanoliter high throughput quantitative PCR
por: Morrison, Tom, et al.
Publicado: (2006)

Quantitative real-time PCR protocol for analysis of nuclear receptor signaling pathways
por: Bookout, Angie L., et al.
Publicado: (2003)

Identification of stable reference genes for quantitative PCR in koalas
por: Sarker, N., et al.
Publicado: (2018)

A rapid and simple quantitative method for specific detection of smaller coterminal RNA by PCR (DeSCo-PCR): application to the detection of viral subgenomic RNAs
por: Kanodia, Pulkit, et al.
Publicado: (2020)

Allele-Specific Quantitative PCR for Accurate, Rapid, and Cost-Effective Genotyping
por: Lee, Han B., et al.
Publicado: (2016)

Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation
por: Whale, Alexandra S., et al.
Publicado: (2012)

Quantitative mitochondrial DNA copy number determination using droplet digital PCR with single-cell resolution
por: O'Hara, Ryan, et al.
Publicado: (2019)

Detection of alternative lengthening of telomeres by telomere quantitative PCR
por: Lau, Loretta M. S., et al.
Publicado: (2013)

qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data
por: Hellemans, Jan, et al.
Publicado: (2007)

Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data
por: Marullo, Manuela, et al.
Publicado: (2010)

Quantitative evaluation of DNA methylation by optimization of a differential-high resolution melt analysis protocol
por: Malentacchi, Francesca, et al.
Publicado: (2009)

Zip nucleic acids are potent hydrolysis probes for quantitative PCR
por: Paris, Clément, et al.
Publicado: (2010)

metaMIC: reference-free misassembly identification and correction of de novo metagenomic assemblies
por: Lai, Senying, et al.
Publicado: (2022)

Evidence‐based selection of reference genes for RT‐qPCR assays in periodontal research
por: Diehl, Daniel, et al.
Publicado: (2022)

Analysis of copy number variation using quantitative interspecies competitive PCR
por: Williams, Nigel M., et al.
Publicado: (2008)

Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data
por: Ruijter, J. M., et al.
Publicado: (2009)

Identification and validation of reference genes for quantitative RT-PCR normalization in wheat
por: Paolacci, Anna R, et al.
Publicado: (2009)

Identification of Reference Genes for Quantitative RT-PCR in Ascending Aortic Aneurysms
por: Henn, Dominic, et al.
Publicado: (2013)

Identification of reference genes for quantitative PCR analyses in developing mouse gonads
por: YOKOYAMA, Toshifumi, et al.
Publicado: (2018)

A new method for robust quantitative and qualitative analysis of real-time PCR
por: Shain, Eric B., et al.
Publicado: (2008)

Telomere length measurement by a novel monochrome multiplex quantitative PCR method
por: Cawthon, Richard M.
Publicado: (2009)

Measurable impact of RNA quality on gene expression results from quantitative PCR
por: Vermeulen, Joëlle, et al.
Publicado: (2011)

Two-Target Quantitative PCR To Predict Library Composition for Shallow Shotgun Sequencing
por: Cho, Matthew Y., et al.
Publicado: (2021)

QDMR: a quantitative method for identification of differentially methylated regions by entropy
por: Zhang, Yan, et al.
Publicado: (2011)

Differentiating Botulinum Neurotoxin-Producing Clostridia with a Simple, Multiplex PCR Assay
por: Williamson, Charles H. D., et al.
Publicado: (2017)

Transfection of the mutant MYH9 cDNA reproduces the most typical cellular phenotype of MYH9-related disease in different cell lines
por: Panza, Emanuele, et al.
Publicado: (2008)

Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinION™ nanopore sequencer
por: Kai, Shinichi, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_q84pbe1g17lcgvl0n501qsjskc