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Photolysis of Staphyloxanthin in Methicillin‐Resistant Staphylococcus aureus Potentiates Killing by Reactive Oxygen Species
Confronted with the severe situation that the pace of resistance acquisition is faster than the clinical introduction of new antibiotics, health organizations are calling for effective approaches to combat methicillin‐resistant Staphylococcus aureus (MRSA) infections. Here, an approach to treat MRSA...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6548961/ https://www.ncbi.nlm.nih.gov/pubmed/31179216 http://dx.doi.org/10.1002/advs.201900030 |
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author | Dong, Pu‐Ting Mohammad, Haroon Hui, Jie Leanse, Leon G. Li, Junjie Liang, Lijia Dai, Tianhong Seleem, Mohamed N. Cheng, Ji‐Xin |
author_facet | Dong, Pu‐Ting Mohammad, Haroon Hui, Jie Leanse, Leon G. Li, Junjie Liang, Lijia Dai, Tianhong Seleem, Mohamed N. Cheng, Ji‐Xin |
author_sort | Dong, Pu‐Ting |
collection | PubMed |
description | Confronted with the severe situation that the pace of resistance acquisition is faster than the clinical introduction of new antibiotics, health organizations are calling for effective approaches to combat methicillin‐resistant Staphylococcus aureus (MRSA) infections. Here, an approach to treat MRSA through photolysis of staphyloxanthin, an antioxidant residing in the microdomain of S. aureus membrane, is reported. This photochemistry process is uncovered through transient absorption imaging and quantitated by absorption spectroscopy, Raman spectroscopy, and mass spectrometry. Photolysis of staphyloxanthin transiently elevates the membrane permeability and renders MRSA highly susceptible to hydrogen peroxide attack. Consequently, staphyloxanthin photolysis by low‐level 460 nm light eradicates MRSA synergistically with hydrogen peroxide and other reactive oxygen species. The effectiveness of this synergistic therapy is well validated in MRSA planktonic culture, MRSA‐infected macrophage cells, stationary‐phase MRSA, persisters, S. aureus biofilms, and two mice wound infection models. Collectively, the work demonstrates that staphyloxanthin photolysis is a new therapeutic platform to treat MRSA infections. |
format | Online Article Text |
id | pubmed-6548961 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-65489612019-06-07 Photolysis of Staphyloxanthin in Methicillin‐Resistant Staphylococcus aureus Potentiates Killing by Reactive Oxygen Species Dong, Pu‐Ting Mohammad, Haroon Hui, Jie Leanse, Leon G. Li, Junjie Liang, Lijia Dai, Tianhong Seleem, Mohamed N. Cheng, Ji‐Xin Adv Sci (Weinh) Full Papers Confronted with the severe situation that the pace of resistance acquisition is faster than the clinical introduction of new antibiotics, health organizations are calling for effective approaches to combat methicillin‐resistant Staphylococcus aureus (MRSA) infections. Here, an approach to treat MRSA through photolysis of staphyloxanthin, an antioxidant residing in the microdomain of S. aureus membrane, is reported. This photochemistry process is uncovered through transient absorption imaging and quantitated by absorption spectroscopy, Raman spectroscopy, and mass spectrometry. Photolysis of staphyloxanthin transiently elevates the membrane permeability and renders MRSA highly susceptible to hydrogen peroxide attack. Consequently, staphyloxanthin photolysis by low‐level 460 nm light eradicates MRSA synergistically with hydrogen peroxide and other reactive oxygen species. The effectiveness of this synergistic therapy is well validated in MRSA planktonic culture, MRSA‐infected macrophage cells, stationary‐phase MRSA, persisters, S. aureus biofilms, and two mice wound infection models. Collectively, the work demonstrates that staphyloxanthin photolysis is a new therapeutic platform to treat MRSA infections. John Wiley and Sons Inc. 2019-03-30 /pmc/articles/PMC6548961/ /pubmed/31179216 http://dx.doi.org/10.1002/advs.201900030 Text en © 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Full Papers Dong, Pu‐Ting Mohammad, Haroon Hui, Jie Leanse, Leon G. Li, Junjie Liang, Lijia Dai, Tianhong Seleem, Mohamed N. Cheng, Ji‐Xin Photolysis of Staphyloxanthin in Methicillin‐Resistant Staphylococcus aureus Potentiates Killing by Reactive Oxygen Species |
title | Photolysis of Staphyloxanthin in Methicillin‐Resistant Staphylococcus aureus Potentiates Killing by Reactive Oxygen Species |
title_full | Photolysis of Staphyloxanthin in Methicillin‐Resistant Staphylococcus aureus Potentiates Killing by Reactive Oxygen Species |
title_fullStr | Photolysis of Staphyloxanthin in Methicillin‐Resistant Staphylococcus aureus Potentiates Killing by Reactive Oxygen Species |
title_full_unstemmed | Photolysis of Staphyloxanthin in Methicillin‐Resistant Staphylococcus aureus Potentiates Killing by Reactive Oxygen Species |
title_short | Photolysis of Staphyloxanthin in Methicillin‐Resistant Staphylococcus aureus Potentiates Killing by Reactive Oxygen Species |
title_sort | photolysis of staphyloxanthin in methicillin‐resistant staphylococcus aureus potentiates killing by reactive oxygen species |
topic | Full Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6548961/ https://www.ncbi.nlm.nih.gov/pubmed/31179216 http://dx.doi.org/10.1002/advs.201900030 |
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