Silencing microRNA-27a inhibits proliferation and invasion of human osteosarcoma cells through the SFRP1-dependent Wnt/β-catenin signaling pathway
Osteosarcoma is the most common malignant tumor of bone with a high potential for metastasis. Importantly, microRNA-27a (miR-27a) is involved in the progression of osteosarcoma. The present study aims to discuss the effects of miR-27a and its target gene secreted frizzled related protein 1 (SFRP1) o...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6549093/ https://www.ncbi.nlm.nih.gov/pubmed/31072914 http://dx.doi.org/10.1042/BSR20182366 |
_version_ | 1783423935272976384 |
---|---|
author | Mu, Yu Zhang, Lina Chen, Xue Chen, Si Shi, Yuanyuan Li, Junfeng |
author_facet | Mu, Yu Zhang, Lina Chen, Xue Chen, Si Shi, Yuanyuan Li, Junfeng |
author_sort | Mu, Yu |
collection | PubMed |
description | Osteosarcoma is the most common malignant tumor of bone with a high potential for metastasis. Importantly, microRNA-27a (miR-27a) is involved in the progression of osteosarcoma. The present study aims to discuss the effects of miR-27a and its target gene secreted frizzled related protein 1 (SFRP1) on proliferation and invasion of human osteosarcoma cells via Wnt/β-catenin signaling pathway. The expression of miR-27a and SFRP1 in osteosarcoma tissues and cells was detected, followed by identification of their relations. Subsequently, miR-27a mimic, miR-27a inhibitor, or siRNA against SFRP1 were introduced into cells (HOS and U2OS) to investigate their role in cell proliferation and invasion. The expression of Wnt/β-catenin signaling pathway-related gene was analyzed to further uncover the regulatory mechanism of miR-27a. The osteosarcoma tissues and cells exhibited elevated miR-27 expression and reduced SFRP1 expression. SFRP1 was verified to be a target gene of miR-27a. Meanwhile, silenced miR-27a inhibited proliferation and invasion of human osteosarcoma cells. Finally, silencing miR-27a inhibited the activation of Wnt/β-catenin signaling pathway, evidenced by reduced β-catenin expression. Our study draws a conclusion that silencing miR-27a dampens osteosarcoma progression, which might be achieved through the inactivation of the Wnt/β-catenin signaling pathway by up-regulating SFRP1. |
format | Online Article Text |
id | pubmed-6549093 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-65490932019-06-18 Silencing microRNA-27a inhibits proliferation and invasion of human osteosarcoma cells through the SFRP1-dependent Wnt/β-catenin signaling pathway Mu, Yu Zhang, Lina Chen, Xue Chen, Si Shi, Yuanyuan Li, Junfeng Biosci Rep Research Articles Osteosarcoma is the most common malignant tumor of bone with a high potential for metastasis. Importantly, microRNA-27a (miR-27a) is involved in the progression of osteosarcoma. The present study aims to discuss the effects of miR-27a and its target gene secreted frizzled related protein 1 (SFRP1) on proliferation and invasion of human osteosarcoma cells via Wnt/β-catenin signaling pathway. The expression of miR-27a and SFRP1 in osteosarcoma tissues and cells was detected, followed by identification of their relations. Subsequently, miR-27a mimic, miR-27a inhibitor, or siRNA against SFRP1 were introduced into cells (HOS and U2OS) to investigate their role in cell proliferation and invasion. The expression of Wnt/β-catenin signaling pathway-related gene was analyzed to further uncover the regulatory mechanism of miR-27a. The osteosarcoma tissues and cells exhibited elevated miR-27 expression and reduced SFRP1 expression. SFRP1 was verified to be a target gene of miR-27a. Meanwhile, silenced miR-27a inhibited proliferation and invasion of human osteosarcoma cells. Finally, silencing miR-27a inhibited the activation of Wnt/β-catenin signaling pathway, evidenced by reduced β-catenin expression. Our study draws a conclusion that silencing miR-27a dampens osteosarcoma progression, which might be achieved through the inactivation of the Wnt/β-catenin signaling pathway by up-regulating SFRP1. Portland Press Ltd. 2019-06-04 /pmc/articles/PMC6549093/ /pubmed/31072914 http://dx.doi.org/10.1042/BSR20182366 Text en © 2019 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Mu, Yu Zhang, Lina Chen, Xue Chen, Si Shi, Yuanyuan Li, Junfeng Silencing microRNA-27a inhibits proliferation and invasion of human osteosarcoma cells through the SFRP1-dependent Wnt/β-catenin signaling pathway |
title | Silencing microRNA-27a inhibits proliferation and invasion of human osteosarcoma cells through the SFRP1-dependent Wnt/β-catenin signaling pathway |
title_full | Silencing microRNA-27a inhibits proliferation and invasion of human osteosarcoma cells through the SFRP1-dependent Wnt/β-catenin signaling pathway |
title_fullStr | Silencing microRNA-27a inhibits proliferation and invasion of human osteosarcoma cells through the SFRP1-dependent Wnt/β-catenin signaling pathway |
title_full_unstemmed | Silencing microRNA-27a inhibits proliferation and invasion of human osteosarcoma cells through the SFRP1-dependent Wnt/β-catenin signaling pathway |
title_short | Silencing microRNA-27a inhibits proliferation and invasion of human osteosarcoma cells through the SFRP1-dependent Wnt/β-catenin signaling pathway |
title_sort | silencing microrna-27a inhibits proliferation and invasion of human osteosarcoma cells through the sfrp1-dependent wnt/β-catenin signaling pathway |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6549093/ https://www.ncbi.nlm.nih.gov/pubmed/31072914 http://dx.doi.org/10.1042/BSR20182366 |
work_keys_str_mv | AT muyu silencingmicrorna27ainhibitsproliferationandinvasionofhumanosteosarcomacellsthroughthesfrp1dependentwntbcateninsignalingpathway AT zhanglina silencingmicrorna27ainhibitsproliferationandinvasionofhumanosteosarcomacellsthroughthesfrp1dependentwntbcateninsignalingpathway AT chenxue silencingmicrorna27ainhibitsproliferationandinvasionofhumanosteosarcomacellsthroughthesfrp1dependentwntbcateninsignalingpathway AT chensi silencingmicrorna27ainhibitsproliferationandinvasionofhumanosteosarcomacellsthroughthesfrp1dependentwntbcateninsignalingpathway AT shiyuanyuan silencingmicrorna27ainhibitsproliferationandinvasionofhumanosteosarcomacellsthroughthesfrp1dependentwntbcateninsignalingpathway AT lijunfeng silencingmicrorna27ainhibitsproliferationandinvasionofhumanosteosarcomacellsthroughthesfrp1dependentwntbcateninsignalingpathway |