Investigation of the clinical significance and prospective molecular mechanisms of cystatin genes in patients with hepatitis B virus-related hepatocellular carcinoma

The present study aimed to investigate the clinical significance and prospective molecular mechanism of cystatin (CST) genes in patients with hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). The role of CST genes in the molecular mechanism of HCC was revealed through bioinformatics an...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Xin, Wang, Xiangkun, Huang, Ketuan, Liao, Xiwen, Yang, Chengkun, Yu, Tingdong, Liu, Junqi, Han, Chuangye, Zhu, Guangzhi, Su, Hao, Qin, Wei, Han, Quanfa, Liu, Zhengqian, Huang, Jianlv, Gong, Yizhen, Ye, Xinping, Peng, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6549101/
https://www.ncbi.nlm.nih.gov/pubmed/31115549
http://dx.doi.org/10.3892/or.2019.7154
_version_ 1783423936908754944
author Zhou, Xin
Wang, Xiangkun
Huang, Ketuan
Liao, Xiwen
Yang, Chengkun
Yu, Tingdong
Liu, Junqi
Han, Chuangye
Zhu, Guangzhi
Su, Hao
Qin, Wei
Han, Quanfa
Liu, Zhengqian
Huang, Jianlv
Gong, Yizhen
Ye, Xinping
Peng, Tao
author_facet Zhou, Xin
Wang, Xiangkun
Huang, Ketuan
Liao, Xiwen
Yang, Chengkun
Yu, Tingdong
Liu, Junqi
Han, Chuangye
Zhu, Guangzhi
Su, Hao
Qin, Wei
Han, Quanfa
Liu, Zhengqian
Huang, Jianlv
Gong, Yizhen
Ye, Xinping
Peng, Tao
author_sort Zhou, Xin
collection PubMed
description The present study aimed to investigate the clinical significance and prospective molecular mechanism of cystatin (CST) genes in patients with hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). The role of CST genes in the molecular mechanism of HCC was revealed through bioinformatics analysis. The clinical significance of CST genes was investigated using GSE14520-derived data from patients with HBV-related HCC. Gene set enrichment analysis (GSEA) was used to identify pathways in which the CST genes were enriched, as well as the association between these pathways and HCC. The expression levels of CST1, CST2, CST5, CSTA and CSTB genes were higher in HCC tissue compared with in normal tissue; conversely, CST3 and CST7 were reduced in HCC tissue. Subsequent receiver operating characteristic analysis of the CST genes demonstrated that CST7 and CSTB genes may function as potential diagnostic markers for HCC. Furthermore, the expression levels of CST6 and CST7 were strongly associated with recurrence-free survival and overall survival of patients with HBV-related HCC. GSEA of the CST genes revealed that CST7 was significantly enriched in tumor evasion and tolerogenicity, cancer progenitors, liver cancer late recurrence, liver cancer progression and several liver cancer subclasses. In addition, CST genes demonstrated homology in terms of protein structure and were revealed to be strongly co-expressed. The present findings suggested that CST7 and CSTB genes may serve as potential prognostic and diagnostic biomarkers for HCC.
format Online
Article
Text
id pubmed-6549101
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-65491012019-06-10 Investigation of the clinical significance and prospective molecular mechanisms of cystatin genes in patients with hepatitis B virus-related hepatocellular carcinoma Zhou, Xin Wang, Xiangkun Huang, Ketuan Liao, Xiwen Yang, Chengkun Yu, Tingdong Liu, Junqi Han, Chuangye Zhu, Guangzhi Su, Hao Qin, Wei Han, Quanfa Liu, Zhengqian Huang, Jianlv Gong, Yizhen Ye, Xinping Peng, Tao Oncol Rep Articles The present study aimed to investigate the clinical significance and prospective molecular mechanism of cystatin (CST) genes in patients with hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). The role of CST genes in the molecular mechanism of HCC was revealed through bioinformatics analysis. The clinical significance of CST genes was investigated using GSE14520-derived data from patients with HBV-related HCC. Gene set enrichment analysis (GSEA) was used to identify pathways in which the CST genes were enriched, as well as the association between these pathways and HCC. The expression levels of CST1, CST2, CST5, CSTA and CSTB genes were higher in HCC tissue compared with in normal tissue; conversely, CST3 and CST7 were reduced in HCC tissue. Subsequent receiver operating characteristic analysis of the CST genes demonstrated that CST7 and CSTB genes may function as potential diagnostic markers for HCC. Furthermore, the expression levels of CST6 and CST7 were strongly associated with recurrence-free survival and overall survival of patients with HBV-related HCC. GSEA of the CST genes revealed that CST7 was significantly enriched in tumor evasion and tolerogenicity, cancer progenitors, liver cancer late recurrence, liver cancer progression and several liver cancer subclasses. In addition, CST genes demonstrated homology in terms of protein structure and were revealed to be strongly co-expressed. The present findings suggested that CST7 and CSTB genes may serve as potential prognostic and diagnostic biomarkers for HCC. D.A. Spandidos 2019-07 2019-05-09 /pmc/articles/PMC6549101/ /pubmed/31115549 http://dx.doi.org/10.3892/or.2019.7154 Text en Copyright: © Zhou et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhou, Xin
Wang, Xiangkun
Huang, Ketuan
Liao, Xiwen
Yang, Chengkun
Yu, Tingdong
Liu, Junqi
Han, Chuangye
Zhu, Guangzhi
Su, Hao
Qin, Wei
Han, Quanfa
Liu, Zhengqian
Huang, Jianlv
Gong, Yizhen
Ye, Xinping
Peng, Tao
Investigation of the clinical significance and prospective molecular mechanisms of cystatin genes in patients with hepatitis B virus-related hepatocellular carcinoma
title Investigation of the clinical significance and prospective molecular mechanisms of cystatin genes in patients with hepatitis B virus-related hepatocellular carcinoma
title_full Investigation of the clinical significance and prospective molecular mechanisms of cystatin genes in patients with hepatitis B virus-related hepatocellular carcinoma
title_fullStr Investigation of the clinical significance and prospective molecular mechanisms of cystatin genes in patients with hepatitis B virus-related hepatocellular carcinoma
title_full_unstemmed Investigation of the clinical significance and prospective molecular mechanisms of cystatin genes in patients with hepatitis B virus-related hepatocellular carcinoma
title_short Investigation of the clinical significance and prospective molecular mechanisms of cystatin genes in patients with hepatitis B virus-related hepatocellular carcinoma
title_sort investigation of the clinical significance and prospective molecular mechanisms of cystatin genes in patients with hepatitis b virus-related hepatocellular carcinoma
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6549101/
https://www.ncbi.nlm.nih.gov/pubmed/31115549
http://dx.doi.org/10.3892/or.2019.7154
work_keys_str_mv AT zhouxin investigationoftheclinicalsignificanceandprospectivemolecularmechanismsofcystatingenesinpatientswithhepatitisbvirusrelatedhepatocellularcarcinoma
AT wangxiangkun investigationoftheclinicalsignificanceandprospectivemolecularmechanismsofcystatingenesinpatientswithhepatitisbvirusrelatedhepatocellularcarcinoma
AT huangketuan investigationoftheclinicalsignificanceandprospectivemolecularmechanismsofcystatingenesinpatientswithhepatitisbvirusrelatedhepatocellularcarcinoma
AT liaoxiwen investigationoftheclinicalsignificanceandprospectivemolecularmechanismsofcystatingenesinpatientswithhepatitisbvirusrelatedhepatocellularcarcinoma
AT yangchengkun investigationoftheclinicalsignificanceandprospectivemolecularmechanismsofcystatingenesinpatientswithhepatitisbvirusrelatedhepatocellularcarcinoma
AT yutingdong investigationoftheclinicalsignificanceandprospectivemolecularmechanismsofcystatingenesinpatientswithhepatitisbvirusrelatedhepatocellularcarcinoma
AT liujunqi investigationoftheclinicalsignificanceandprospectivemolecularmechanismsofcystatingenesinpatientswithhepatitisbvirusrelatedhepatocellularcarcinoma
AT hanchuangye investigationoftheclinicalsignificanceandprospectivemolecularmechanismsofcystatingenesinpatientswithhepatitisbvirusrelatedhepatocellularcarcinoma
AT zhuguangzhi investigationoftheclinicalsignificanceandprospectivemolecularmechanismsofcystatingenesinpatientswithhepatitisbvirusrelatedhepatocellularcarcinoma
AT suhao investigationoftheclinicalsignificanceandprospectivemolecularmechanismsofcystatingenesinpatientswithhepatitisbvirusrelatedhepatocellularcarcinoma
AT qinwei investigationoftheclinicalsignificanceandprospectivemolecularmechanismsofcystatingenesinpatientswithhepatitisbvirusrelatedhepatocellularcarcinoma
AT hanquanfa investigationoftheclinicalsignificanceandprospectivemolecularmechanismsofcystatingenesinpatientswithhepatitisbvirusrelatedhepatocellularcarcinoma
AT liuzhengqian investigationoftheclinicalsignificanceandprospectivemolecularmechanismsofcystatingenesinpatientswithhepatitisbvirusrelatedhepatocellularcarcinoma
AT huangjianlv investigationoftheclinicalsignificanceandprospectivemolecularmechanismsofcystatingenesinpatientswithhepatitisbvirusrelatedhepatocellularcarcinoma
AT gongyizhen investigationoftheclinicalsignificanceandprospectivemolecularmechanismsofcystatingenesinpatientswithhepatitisbvirusrelatedhepatocellularcarcinoma
AT yexinping investigationoftheclinicalsignificanceandprospectivemolecularmechanismsofcystatingenesinpatientswithhepatitisbvirusrelatedhepatocellularcarcinoma
AT pengtao investigationoftheclinicalsignificanceandprospectivemolecularmechanismsofcystatingenesinpatientswithhepatitisbvirusrelatedhepatocellularcarcinoma