Cargando…

Development of a rapid, simple and efficient one-pot cloning method for a reverse genetics system of broad subtypes of influenza A virus

The reverse genetics (RG) system of influenza A viruses is well established. However, the conventional sequence-dependent method for cloning influenza genome segments is time-consuming and requires multiple processes (eg. enzyme digestion and ligation) and exhibits low cloning efficiency compared to...

Descripción completa

Detalles Bibliográficos
Autores principales:	Choi, Won-Suk, Jeong, Ju Hwan, Lloren, Khristine Kaith S., Ahn, Su Jeong, Antigua, Khristine Joy C., Kim, Young-il, Si, Young-Jae, Baek, Yun Hee, Choi, Young Ki, Song, Min-Suk
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6549168/ https://www.ncbi.nlm.nih.gov/pubmed/31165766 http://dx.doi.org/10.1038/s41598-019-44813-z

Ejemplares similares

Rapid and simple colorimetric detection of multiple influenza viruses infecting humans using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform
por: Ahn, Su Jeong, et al.
Publicado: (2019)

Correction to: Rapid and simple colorimetric detection of multiple influenza viruses infecting humans using a reverse transcriptional loopmediated isothermal amplification (RT-LAMP) diagnostic platform
por: Ahn, Su Jeong, et al.
Publicado: (2020)

The significance of avian influenza virus mouse-adaptation and its application in characterizing the efficacy of new vaccines and therapeutic agents
por: Choi, Won-Suk, et al.
Publicado: (2017)

Molecular Markers for Interspecies Transmission of Avian Influenza Viruses in Mammalian Hosts
por: Lloren, Khristine Kaith S., et al.
Publicado: (2017)

The Emergence and Decennary Distribution of Clade 2.3.4.4 HPAI H5Nx
por: Antigua, Khristine Joy C., et al.
Publicado: (2019)

Development of a Universal Cloning System for Reverse Genetics of Human Enteroviruses
por: Choi, Won-Suk, et al.
Publicado: (2023)

Structure, Substrate Specificity and Role of Lon Protease in Bacterial Pathogenesis and Survival
por: Kirthika, Perumalraja, et al.
Publicado: (2023)

Multiple HA substitutions in highly pathogenic avian influenza H5Nx viruses contributed to the change in the NA subtype preference
por: Antigua, Khristine Joy C., et al.
Publicado: (2022)

Salmonella-mediated oral delivery of multiple-target vaccine constructs with conserved and variable regions of SARS-CoV-2 protect against the Delta and Omicron variants in hamster
por: Lloren, Khristine Kaith Sison, et al.
Publicado: (2023)

An mRNA-Based Multiple Antigenic Gene Expression System Delivered by Engineered Salmonella for Severe Fever with Thrombocytopenia Syndrome and Assessment of Its Immunogenicity and Protection Using a Human DC-SIGN-Transduced Mouse Model
por: Park, Ji-Young, et al.
Publicado: (2023)

Development of a reverse transcription-loop-mediated isothermal amplification as a rapid early-detection method for novel SARS-CoV-2
por: Baek, Yun Hee, et al.
Publicado: (2020)

Peptide Nucleic Acid (PNA)-Enhanced Specificity of a Dual-Target Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) Assay for the Detection and Differentiation of SARS-CoV-2 from Related Viruses
por: Choi, Won-Suk, et al.
Publicado: (2020)

One-Pot Reverse Transcriptional Loop-Mediated Isothermal Amplification (RT-LAMP) for Detecting MERS-CoV
por: Lee, Se Hee, et al.
Publicado: (2017)

Macromedia Dreamweaver MX : training from the source /
por: Page, Khristine Annwn
Publicado: (2003)

Macromedia Dreamweaver MX 2004: training from the source
por: Page, Khristine Annwn
Publicado: (2004)

Macromedia Dreamweaver MX: training from the source
por: Page, Khristine Annwn
Publicado: (2003)

Macromedia Dreamweaver 8
por: Page, Khristine Annwn
Publicado: (2006)

Macromedia Dreamweaver 4: training from the source
por: Page, Khristine Annwn, et al.
Publicado: (2001)

A Cross-sectional Analysis of Facebook Comments to Study Public Perception of the Mass Drug Administration Program in the Philippines
por: Labana, Ryan V., et al.
Publicado: (2020)

Universal size ratios of Gaussian polymers with complex architecture: radius of gyration vs hydrodynamic radius
por: Haydukivska, Khristine, et al.
Publicado: (2020)

Insights from one thousand cloned dogs
por: Olsson, P. Olof, et al.
Publicado: (2022)

Efficacy of tamoxifen for the treatment of severe equine asthma
por: Mainguy‐Seers, Sophie, et al.
Publicado: (2018)

Nebulized dexamethasone sodium phosphate in the treatment of horses with severe asthma
por: de Wasseige, Selena, et al.
Publicado: (2021)

A Rapid Method for Generating Infectious SARS-CoV-2 and Variants Using Mutagenesis and Circular Polymerase Extension Cloning
por: Kim, Beom Kyu, et al.
Publicado: (2023)

Effects of sterilization methods on the survival of pathogenic bacteria in potting soil stored at various temperatures
por: Hyun, Jeong-Eun, et al.
Publicado: (2022)

Dahl (S × R) Rat Congenic Strain Analysis Confirms and Defines a Chromosome 17 Spatial Navigation Quantitative Trait Locus to <10 Mbp
por: Herrera, Victoria L., et al.
Publicado: (2013)

Expression of MUC5AC and Trefoil Peptide 1 (TFF1) in the Subtypes of Intestinal Metaplasia
por: Song, Joo-Yong, et al.
Publicado: (2012)

Simple surgical punctal occlusion with high frequency radiowave electrosurgery
por: Park, Jeong Woo, et al.
Publicado: (2023)

Dahl (S x R) Congenic Strain Analysis Confirms and Defines a Chromosome 5 Female-Specific Blood Pressure Quantitative Trait Locus to <7 Mbp
por: Herrera, Victoria L. M., et al.
Publicado: (2012)

Differential Genetic Basis for Pre-Menopausal and Post-Menopausal Salt-Sensitive Hypertension
por: Herrera, Victoria L. M., et al.
Publicado: (2012)

Worse Renal Disease in Postmenopausal F2[Dahl S x R]-Intercross Rats: Detection of Novel QTLs Affecting Hypertensive Kidney Disease
por: Herrera, Victoria L. M., et al.
Publicado: (2013)

Zika virus lateral flow assays using reverse transcription-loop-mediated isothermal amplification
por: Ahn, Gna, et al.
Publicado: (2021)

The Standardized BHH10 Extract, a Combination of Astragalus membranaceus, Cinnamomum cassia, and Phellodendron amurense, Reverses Bone Mass and Metabolism in a Rat Model of Postmenopausal Osteoporosis
por: Huh, Jeong-Eun, et al.
Publicado: (2015)

Histologic subtypes are not associated with the presence of sarcopenia in lung cancer
por: Kim, Chang Rae, et al.
Publicado: (2018)

Simple and Versatile Molecular Method of Copy-Number Measurement Using Cloned Competitors
por: Kim, Hyun-Kyoung, et al.
Publicado: (2013)

The Resurrection of Mabrokan: Production of Multiple Cloned Offspring from Decade-Old Vitrified Tissue Collected from a Deceased Champion Show Camel
por: Hossein, Mohammad Shamim, et al.
Publicado: (2021)

Development of a Rapid Fluorescent Diagnostic System to Detect Subtype H9 Influenza A Virus in Chicken Feces
por: Tuong, Hien Thi, et al.
Publicado: (2021)

Development and pregnancy rates of Camelus dromedarius-cloned embryos derived from in vivo- and in vitro-matured oocytes
por: Son, Young-Bum, et al.
Publicado: (2022)

A Reverse Genetics System for Zika Virus Based on a Simple Molecular Cloning Strategy
por: Münster, Maximilian, et al.
Publicado: (2018)

Cloning and Characterization of an Endoglucanase Gene from Actinomyces sp. Korean Native Goat 40
por: Kim, Sung Chan, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_ogr5csuuh8mc0tpo8jr5r1542v