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Developing regulatory property of gelatin-tannic acid multilayer films for coating-based nitric oxide gas delivery system

To utilize potentials of nitric oxide (NO) gas in anti-bacterial, anticancer, wound healing applications, numerous studies have been conducted to develop a NO delivery system in the past few decades. Even though a coating method and film types are essential to apply in biomedical device coating from...

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Autores principales: Park, Kyungtae, Jeong, Hyejoong, Tanum, Junjira, Yoo, Jae-chan, Hong, Jinkee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6549184/
https://www.ncbi.nlm.nih.gov/pubmed/31165751
http://dx.doi.org/10.1038/s41598-019-44678-2
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author Park, Kyungtae
Jeong, Hyejoong
Tanum, Junjira
Yoo, Jae-chan
Hong, Jinkee
author_facet Park, Kyungtae
Jeong, Hyejoong
Tanum, Junjira
Yoo, Jae-chan
Hong, Jinkee
author_sort Park, Kyungtae
collection PubMed
description To utilize potentials of nitric oxide (NO) gas in anti-bacterial, anticancer, wound healing applications, numerous studies have been conducted to develop a NO delivery system in the past few decades. Even though a coating method and film types are essential to apply in biomedical device coating from previous NO delivery systems, release control from the coating system is still challenging. In this study, we introduced a multilayered polymeric coating system to overcome the uncontrollable NO release kinetics of film systems. We used biocompatible gelatin and tannic acid to construct a rough, porous structured film based on the layer-by-layer self-assembly method. The multilayered polymeric structure facilitated the controlled amount of NO release from (Gel/TA)(n) film and showed burst release in early period owing to their large surface area from the rough, porous structure. We synthesized the proton-responsive NO donor, N-diazeniumdiolate (NONOates), into the (Gel/TA)(n) film through a chemical reaction under high pressure NO gas. NO release profile was analyzed by a real-time NO analysis machine (NOA 280i). Then, the NO-releasing (Gel/TA)(n) film was tested its toxicity against human dermal fibroblast cells and bactericidal effects against Staphylococcus aureus.
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spelling pubmed-65491842019-06-12 Developing regulatory property of gelatin-tannic acid multilayer films for coating-based nitric oxide gas delivery system Park, Kyungtae Jeong, Hyejoong Tanum, Junjira Yoo, Jae-chan Hong, Jinkee Sci Rep Article To utilize potentials of nitric oxide (NO) gas in anti-bacterial, anticancer, wound healing applications, numerous studies have been conducted to develop a NO delivery system in the past few decades. Even though a coating method and film types are essential to apply in biomedical device coating from previous NO delivery systems, release control from the coating system is still challenging. In this study, we introduced a multilayered polymeric coating system to overcome the uncontrollable NO release kinetics of film systems. We used biocompatible gelatin and tannic acid to construct a rough, porous structured film based on the layer-by-layer self-assembly method. The multilayered polymeric structure facilitated the controlled amount of NO release from (Gel/TA)(n) film and showed burst release in early period owing to their large surface area from the rough, porous structure. We synthesized the proton-responsive NO donor, N-diazeniumdiolate (NONOates), into the (Gel/TA)(n) film through a chemical reaction under high pressure NO gas. NO release profile was analyzed by a real-time NO analysis machine (NOA 280i). Then, the NO-releasing (Gel/TA)(n) film was tested its toxicity against human dermal fibroblast cells and bactericidal effects against Staphylococcus aureus. Nature Publishing Group UK 2019-06-05 /pmc/articles/PMC6549184/ /pubmed/31165751 http://dx.doi.org/10.1038/s41598-019-44678-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Park, Kyungtae
Jeong, Hyejoong
Tanum, Junjira
Yoo, Jae-chan
Hong, Jinkee
Developing regulatory property of gelatin-tannic acid multilayer films for coating-based nitric oxide gas delivery system
title Developing regulatory property of gelatin-tannic acid multilayer films for coating-based nitric oxide gas delivery system
title_full Developing regulatory property of gelatin-tannic acid multilayer films for coating-based nitric oxide gas delivery system
title_fullStr Developing regulatory property of gelatin-tannic acid multilayer films for coating-based nitric oxide gas delivery system
title_full_unstemmed Developing regulatory property of gelatin-tannic acid multilayer films for coating-based nitric oxide gas delivery system
title_short Developing regulatory property of gelatin-tannic acid multilayer films for coating-based nitric oxide gas delivery system
title_sort developing regulatory property of gelatin-tannic acid multilayer films for coating-based nitric oxide gas delivery system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6549184/
https://www.ncbi.nlm.nih.gov/pubmed/31165751
http://dx.doi.org/10.1038/s41598-019-44678-2
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