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Multi-omics Analysis of Gut Microbiota and Metabolites in Rats With Irritable Bowel Syndrome
Irritable bowel syndrome (IBS) is a common gastrointestinal dysfunctional disease. The pathophysiology of IBS is, however, largely unknown. This study aimed to determine whether evaluation of fecal metabolite and microbiota profiles may offer an opportunity to identify a novel pathophysiological tar...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6549239/ https://www.ncbi.nlm.nih.gov/pubmed/31192167 http://dx.doi.org/10.3389/fcimb.2019.00178 |
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author | Liu, Si Si, Chaozeng Yu, Yang Zhao, Guiping Chen, Lei Zhao, Yu Zhang, Zheng Li, Hengcun Chen, Yang Min, Li Zhang, Shutian Zhu, Shengtao |
author_facet | Liu, Si Si, Chaozeng Yu, Yang Zhao, Guiping Chen, Lei Zhao, Yu Zhang, Zheng Li, Hengcun Chen, Yang Min, Li Zhang, Shutian Zhu, Shengtao |
author_sort | Liu, Si |
collection | PubMed |
description | Irritable bowel syndrome (IBS) is a common gastrointestinal dysfunctional disease. The pathophysiology of IBS is, however, largely unknown. This study aimed to determine whether evaluation of fecal metabolite and microbiota profiles may offer an opportunity to identify a novel pathophysiological target for IBS, and to reveal possible gut microbe–metabolite associations. By using gas chromatography coupled to time-of-flight mass spectrometry (GC-TOFMS) and 16S rRNA gene sequencing, we measured fecal metabolites and microbiota of the control and water avoidance stress (WAS)-induced IBS rats. We found a significantly differential metabolite profile between the IBS and control groups; a cluster of metabolites was also found to be significantly associated with the amount of defecations. Moreover, the WAS group exhibited a decreased alpha diversity of the microbial population as compared to the control group. However, the characteristics of gut microbiota could not differentiate the IBS group from the control group. Correlation of the metabolite level with the number of microbial genera showed no significant association between the control and IBS groups. This study provides a global perspective on metabolomics and microbiota profiling in WAS-induced IBS model and a theoretical basis for research on the pathophysiology of IBS. |
format | Online Article Text |
id | pubmed-6549239 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-65492392019-06-12 Multi-omics Analysis of Gut Microbiota and Metabolites in Rats With Irritable Bowel Syndrome Liu, Si Si, Chaozeng Yu, Yang Zhao, Guiping Chen, Lei Zhao, Yu Zhang, Zheng Li, Hengcun Chen, Yang Min, Li Zhang, Shutian Zhu, Shengtao Front Cell Infect Microbiol Cellular and Infection Microbiology Irritable bowel syndrome (IBS) is a common gastrointestinal dysfunctional disease. The pathophysiology of IBS is, however, largely unknown. This study aimed to determine whether evaluation of fecal metabolite and microbiota profiles may offer an opportunity to identify a novel pathophysiological target for IBS, and to reveal possible gut microbe–metabolite associations. By using gas chromatography coupled to time-of-flight mass spectrometry (GC-TOFMS) and 16S rRNA gene sequencing, we measured fecal metabolites and microbiota of the control and water avoidance stress (WAS)-induced IBS rats. We found a significantly differential metabolite profile between the IBS and control groups; a cluster of metabolites was also found to be significantly associated with the amount of defecations. Moreover, the WAS group exhibited a decreased alpha diversity of the microbial population as compared to the control group. However, the characteristics of gut microbiota could not differentiate the IBS group from the control group. Correlation of the metabolite level with the number of microbial genera showed no significant association between the control and IBS groups. This study provides a global perspective on metabolomics and microbiota profiling in WAS-induced IBS model and a theoretical basis for research on the pathophysiology of IBS. Frontiers Media S.A. 2019-05-29 /pmc/articles/PMC6549239/ /pubmed/31192167 http://dx.doi.org/10.3389/fcimb.2019.00178 Text en Copyright © 2019 Liu, Si, Yu, Zhao, Chen, Zhao, Zhang, Li, Chen, Min, Zhang and Zhu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Liu, Si Si, Chaozeng Yu, Yang Zhao, Guiping Chen, Lei Zhao, Yu Zhang, Zheng Li, Hengcun Chen, Yang Min, Li Zhang, Shutian Zhu, Shengtao Multi-omics Analysis of Gut Microbiota and Metabolites in Rats With Irritable Bowel Syndrome |
title | Multi-omics Analysis of Gut Microbiota and Metabolites in Rats With Irritable Bowel Syndrome |
title_full | Multi-omics Analysis of Gut Microbiota and Metabolites in Rats With Irritable Bowel Syndrome |
title_fullStr | Multi-omics Analysis of Gut Microbiota and Metabolites in Rats With Irritable Bowel Syndrome |
title_full_unstemmed | Multi-omics Analysis of Gut Microbiota and Metabolites in Rats With Irritable Bowel Syndrome |
title_short | Multi-omics Analysis of Gut Microbiota and Metabolites in Rats With Irritable Bowel Syndrome |
title_sort | multi-omics analysis of gut microbiota and metabolites in rats with irritable bowel syndrome |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6549239/ https://www.ncbi.nlm.nih.gov/pubmed/31192167 http://dx.doi.org/10.3389/fcimb.2019.00178 |
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