Fluid shear stress induces cell migration and invasion via activating autophagy in HepG2 cells

Fluid shear stress (FSS) regulates the metastasis of hepatocellular carcinoma (HCC). In the present study, we aimed to study the role of autophagy in HCC cells under FSS. The results showed that FSS upregulated the protein markers of autophagy, induced LC3B aggregation and formation of autophagosome...

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Autores principales: Yan, Zhiping, Su, Guanyue, Gao, Wenbo, He, Jia, Shen, Yang, Zeng, Ye, Liu, Xiaoheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6550537/
https://www.ncbi.nlm.nih.gov/pubmed/30663937
http://dx.doi.org/10.1080/19336918.2019.1568141
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author Yan, Zhiping
Su, Guanyue
Gao, Wenbo
He, Jia
Shen, Yang
Zeng, Ye
Liu, Xiaoheng
author_facet Yan, Zhiping
Su, Guanyue
Gao, Wenbo
He, Jia
Shen, Yang
Zeng, Ye
Liu, Xiaoheng
author_sort Yan, Zhiping
collection PubMed
description Fluid shear stress (FSS) regulates the metastasis of hepatocellular carcinoma (HCC). In the present study, we aimed to study the role of autophagy in HCC cells under FSS. The results showed that FSS upregulated the protein markers of autophagy, induced LC3B aggregation and formation of autophagosomes. Inhibition of integrin by Cliengitide (Cli) or inhibition of the microfilaments formation both inhibited the activation of autophagy in HepG2 under FSS. In addition, Cli inhibited the microfilaments formation and expressions of Rac1 and RhoA in HepG2 cells under FSS. Finally, inhibition of autophagy suppressed the cell migration and invasion in HepG2 under FSS. In conclusion, FSS induced autophagy to promote migration and invasion of HepG2 cells via integrin/cytoskeleton pathways.
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spelling pubmed-65505372019-06-17 Fluid shear stress induces cell migration and invasion via activating autophagy in HepG2 cells Yan, Zhiping Su, Guanyue Gao, Wenbo He, Jia Shen, Yang Zeng, Ye Liu, Xiaoheng Cell Adh Migr Research Paper Fluid shear stress (FSS) regulates the metastasis of hepatocellular carcinoma (HCC). In the present study, we aimed to study the role of autophagy in HCC cells under FSS. The results showed that FSS upregulated the protein markers of autophagy, induced LC3B aggregation and formation of autophagosomes. Inhibition of integrin by Cliengitide (Cli) or inhibition of the microfilaments formation both inhibited the activation of autophagy in HepG2 under FSS. In addition, Cli inhibited the microfilaments formation and expressions of Rac1 and RhoA in HepG2 cells under FSS. Finally, inhibition of autophagy suppressed the cell migration and invasion in HepG2 under FSS. In conclusion, FSS induced autophagy to promote migration and invasion of HepG2 cells via integrin/cytoskeleton pathways. Taylor & Francis 2019-01-31 /pmc/articles/PMC6550537/ /pubmed/30663937 http://dx.doi.org/10.1080/19336918.2019.1568141 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Yan, Zhiping
Su, Guanyue
Gao, Wenbo
He, Jia
Shen, Yang
Zeng, Ye
Liu, Xiaoheng
Fluid shear stress induces cell migration and invasion via activating autophagy in HepG2 cells
title Fluid shear stress induces cell migration and invasion via activating autophagy in HepG2 cells
title_full Fluid shear stress induces cell migration and invasion via activating autophagy in HepG2 cells
title_fullStr Fluid shear stress induces cell migration and invasion via activating autophagy in HepG2 cells
title_full_unstemmed Fluid shear stress induces cell migration and invasion via activating autophagy in HepG2 cells
title_short Fluid shear stress induces cell migration and invasion via activating autophagy in HepG2 cells
title_sort fluid shear stress induces cell migration and invasion via activating autophagy in hepg2 cells
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6550537/
https://www.ncbi.nlm.nih.gov/pubmed/30663937
http://dx.doi.org/10.1080/19336918.2019.1568141
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