Platelet endothelial aggregation receptor-1 regulates bovine muscle satellite cell migration and differentiation via integrin beta-1 and focal adhesion kinase

PEAR1 is highly expressed at bovine MDSC differentiation. However, its biological function remains unclear. Western blotting results showed that PEAR1 increased between day 0 and day 2 of cell differentiation and decreased from day 3. Moreover, scratch test showed that wound healing rate increased a...

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Autores principales: Pang, Yusheng, Zhang, Ziheng, Wang, Zhao, Wang, Yuxin, Yan, Yunqin, Li, Shufeng, Tong, Huili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6550786/
https://www.ncbi.nlm.nih.gov/pubmed/31096840
http://dx.doi.org/10.1080/19336918.2019.1619434
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author Pang, Yusheng
Zhang, Ziheng
Wang, Zhao
Wang, Yuxin
Yan, Yunqin
Li, Shufeng
Tong, Huili
author_facet Pang, Yusheng
Zhang, Ziheng
Wang, Zhao
Wang, Yuxin
Yan, Yunqin
Li, Shufeng
Tong, Huili
author_sort Pang, Yusheng
collection PubMed
description PEAR1 is highly expressed at bovine MDSC differentiation. However, its biological function remains unclear. Western blotting results showed that PEAR1 increased between day 0 and day 2 of cell differentiation and decreased from day 3. Moreover, scratch test showed that wound healing rate increased after PEAR1 overexpression and decreased upon its suppression. Meanwhile, we found that, upon PEAR1 induction, both the expression of the focal adhesion-associated and MyoG, and the myotube fusion rate increased. However, when PEAR1 was suppressed, opposite results were obtained. Immunoprecipitation revealed an association between PEAR1 and ITGB1. Notably, inhibition of FAK and ITGB1 repressed cell differentiation. In conclusion, our study indicated that PEAR1 is involved in the regulation of bovine MDSC migration and differentiation.
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spelling pubmed-65507862019-06-17 Platelet endothelial aggregation receptor-1 regulates bovine muscle satellite cell migration and differentiation via integrin beta-1 and focal adhesion kinase Pang, Yusheng Zhang, Ziheng Wang, Zhao Wang, Yuxin Yan, Yunqin Li, Shufeng Tong, Huili Cell Adh Migr Research Paper PEAR1 is highly expressed at bovine MDSC differentiation. However, its biological function remains unclear. Western blotting results showed that PEAR1 increased between day 0 and day 2 of cell differentiation and decreased from day 3. Moreover, scratch test showed that wound healing rate increased after PEAR1 overexpression and decreased upon its suppression. Meanwhile, we found that, upon PEAR1 induction, both the expression of the focal adhesion-associated and MyoG, and the myotube fusion rate increased. However, when PEAR1 was suppressed, opposite results were obtained. Immunoprecipitation revealed an association between PEAR1 and ITGB1. Notably, inhibition of FAK and ITGB1 repressed cell differentiation. In conclusion, our study indicated that PEAR1 is involved in the regulation of bovine MDSC migration and differentiation. Taylor & Francis 2019-05-27 /pmc/articles/PMC6550786/ /pubmed/31096840 http://dx.doi.org/10.1080/19336918.2019.1619434 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Pang, Yusheng
Zhang, Ziheng
Wang, Zhao
Wang, Yuxin
Yan, Yunqin
Li, Shufeng
Tong, Huili
Platelet endothelial aggregation receptor-1 regulates bovine muscle satellite cell migration and differentiation via integrin beta-1 and focal adhesion kinase
title Platelet endothelial aggregation receptor-1 regulates bovine muscle satellite cell migration and differentiation via integrin beta-1 and focal adhesion kinase
title_full Platelet endothelial aggregation receptor-1 regulates bovine muscle satellite cell migration and differentiation via integrin beta-1 and focal adhesion kinase
title_fullStr Platelet endothelial aggregation receptor-1 regulates bovine muscle satellite cell migration and differentiation via integrin beta-1 and focal adhesion kinase
title_full_unstemmed Platelet endothelial aggregation receptor-1 regulates bovine muscle satellite cell migration and differentiation via integrin beta-1 and focal adhesion kinase
title_short Platelet endothelial aggregation receptor-1 regulates bovine muscle satellite cell migration and differentiation via integrin beta-1 and focal adhesion kinase
title_sort platelet endothelial aggregation receptor-1 regulates bovine muscle satellite cell migration and differentiation via integrin beta-1 and focal adhesion kinase
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6550786/
https://www.ncbi.nlm.nih.gov/pubmed/31096840
http://dx.doi.org/10.1080/19336918.2019.1619434
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