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Raising fluid walls around living cells
An effective transformation of the cell culture dishes that biologists use every day into microfluidic devices would open many avenues for miniaturizing cell-based workflows. In this article, we report a simple method for creating microfluidic arrangements around cells already growing on the surface...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6551168/ https://www.ncbi.nlm.nih.gov/pubmed/31183401 http://dx.doi.org/10.1126/sciadv.aav8002 |
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author | Soitu, Cristian Feuerborn, Alexander Deroy, Cyril Castrejón-Pita, Alfonso A. Cook, Peter R. Walsh, Edmond J. |
author_facet | Soitu, Cristian Feuerborn, Alexander Deroy, Cyril Castrejón-Pita, Alfonso A. Cook, Peter R. Walsh, Edmond J. |
author_sort | Soitu, Cristian |
collection | PubMed |
description | An effective transformation of the cell culture dishes that biologists use every day into microfluidic devices would open many avenues for miniaturizing cell-based workflows. In this article, we report a simple method for creating microfluidic arrangements around cells already growing on the surface of standard petri dishes, using the interface between immiscible fluids as a “building material.” Conventional dishes are repurposed into sophisticated microfluidic devices by reshaping, on demand, the fluid structures around living cells. Moreover, these microfluidic arrangements can be further reconfigured during experiments, which is impossible with most existing microfluidic platforms. The method is demonstrated using workflows involving cell cloning, the selection of a particular clone from among others in a dish, drug treatments, and wound healing. The versatility of the approach and its biologically friendly aspects may hasten uptake by biologists of microfluidics, so the technology finally fulfills its potential. |
format | Online Article Text |
id | pubmed-6551168 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-65511682019-06-10 Raising fluid walls around living cells Soitu, Cristian Feuerborn, Alexander Deroy, Cyril Castrejón-Pita, Alfonso A. Cook, Peter R. Walsh, Edmond J. Sci Adv Research Articles An effective transformation of the cell culture dishes that biologists use every day into microfluidic devices would open many avenues for miniaturizing cell-based workflows. In this article, we report a simple method for creating microfluidic arrangements around cells already growing on the surface of standard petri dishes, using the interface between immiscible fluids as a “building material.” Conventional dishes are repurposed into sophisticated microfluidic devices by reshaping, on demand, the fluid structures around living cells. Moreover, these microfluidic arrangements can be further reconfigured during experiments, which is impossible with most existing microfluidic platforms. The method is demonstrated using workflows involving cell cloning, the selection of a particular clone from among others in a dish, drug treatments, and wound healing. The versatility of the approach and its biologically friendly aspects may hasten uptake by biologists of microfluidics, so the technology finally fulfills its potential. American Association for the Advancement of Science 2019-06-05 /pmc/articles/PMC6551168/ /pubmed/31183401 http://dx.doi.org/10.1126/sciadv.aav8002 Text en Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Soitu, Cristian Feuerborn, Alexander Deroy, Cyril Castrejón-Pita, Alfonso A. Cook, Peter R. Walsh, Edmond J. Raising fluid walls around living cells |
title | Raising fluid walls around living cells |
title_full | Raising fluid walls around living cells |
title_fullStr | Raising fluid walls around living cells |
title_full_unstemmed | Raising fluid walls around living cells |
title_short | Raising fluid walls around living cells |
title_sort | raising fluid walls around living cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6551168/ https://www.ncbi.nlm.nih.gov/pubmed/31183401 http://dx.doi.org/10.1126/sciadv.aav8002 |
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