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Molecularly defined cortical astroglia subpopulation modulates neurons via secretion of Norrin

Despite expanding knowledge regarding the role of astroglia in regulating neuronal function, little is known about regional or functional subgroups of brain astroglia and how they may interact with neurons. We use an astroglia-specific promoter fragment in transgenic mice to identify an anatomically...

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Autores principales: Miller, Sean J., Philips, Thomas, Kim, Namho, Dastgheyb, Raha, Chen, Zhuoxun, Hsieh, Yi-Chun, Daigle, J. Gavin, Datta, Malika, Chew, Jeannie, Vidensky, Svetlana, Pham, Jacqueline T., Hughes, Ethan G., Robinson, Michael B., Sattler, Rita, Tomer, Raju, Suk, Jung Soo, Bergles, Dwight E., Haughey, Norman, Pletnikov, Mikhail, Hanes, Justin, Rothstein, Jeffrey D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6551209/
https://www.ncbi.nlm.nih.gov/pubmed/30936556
http://dx.doi.org/10.1038/s41593-019-0366-7
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author Miller, Sean J.
Philips, Thomas
Kim, Namho
Dastgheyb, Raha
Chen, Zhuoxun
Hsieh, Yi-Chun
Daigle, J. Gavin
Datta, Malika
Chew, Jeannie
Vidensky, Svetlana
Pham, Jacqueline T.
Hughes, Ethan G.
Robinson, Michael B.
Sattler, Rita
Tomer, Raju
Suk, Jung Soo
Bergles, Dwight E.
Haughey, Norman
Pletnikov, Mikhail
Hanes, Justin
Rothstein, Jeffrey D.
author_facet Miller, Sean J.
Philips, Thomas
Kim, Namho
Dastgheyb, Raha
Chen, Zhuoxun
Hsieh, Yi-Chun
Daigle, J. Gavin
Datta, Malika
Chew, Jeannie
Vidensky, Svetlana
Pham, Jacqueline T.
Hughes, Ethan G.
Robinson, Michael B.
Sattler, Rita
Tomer, Raju
Suk, Jung Soo
Bergles, Dwight E.
Haughey, Norman
Pletnikov, Mikhail
Hanes, Justin
Rothstein, Jeffrey D.
author_sort Miller, Sean J.
collection PubMed
description Despite expanding knowledge regarding the role of astroglia in regulating neuronal function, little is known about regional or functional subgroups of brain astroglia and how they may interact with neurons. We use an astroglia-specific promoter fragment in transgenic mice to identify an anatomically defined subset of adult grey matter astroglia. Using transcriptomic and histological analyses, we generate a combinatorial profile for in vivo identification and characterization of this astroglia subpopulation. These astroglia are enriched in cortical layer V, express distinct molecular markers, including norrin and LGR6, with corresponding layer-specific neuronal ligands, are found in human cortex and modulate neuronal activity. Astrocytic norrin appears to regulate dendrites and spines and its loss, as occurs in Norrie disease, contributes to cortical dendritic spine loss. These studies provide evidence that human and rodent astroglia subtypes are regionally and functionally distinct, can regulate local neuronal dendrite and synaptic spine development and contribute to disease.
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spelling pubmed-65512092019-10-01 Molecularly defined cortical astroglia subpopulation modulates neurons via secretion of Norrin Miller, Sean J. Philips, Thomas Kim, Namho Dastgheyb, Raha Chen, Zhuoxun Hsieh, Yi-Chun Daigle, J. Gavin Datta, Malika Chew, Jeannie Vidensky, Svetlana Pham, Jacqueline T. Hughes, Ethan G. Robinson, Michael B. Sattler, Rita Tomer, Raju Suk, Jung Soo Bergles, Dwight E. Haughey, Norman Pletnikov, Mikhail Hanes, Justin Rothstein, Jeffrey D. Nat Neurosci Article Despite expanding knowledge regarding the role of astroglia in regulating neuronal function, little is known about regional or functional subgroups of brain astroglia and how they may interact with neurons. We use an astroglia-specific promoter fragment in transgenic mice to identify an anatomically defined subset of adult grey matter astroglia. Using transcriptomic and histological analyses, we generate a combinatorial profile for in vivo identification and characterization of this astroglia subpopulation. These astroglia are enriched in cortical layer V, express distinct molecular markers, including norrin and LGR6, with corresponding layer-specific neuronal ligands, are found in human cortex and modulate neuronal activity. Astrocytic norrin appears to regulate dendrites and spines and its loss, as occurs in Norrie disease, contributes to cortical dendritic spine loss. These studies provide evidence that human and rodent astroglia subtypes are regionally and functionally distinct, can regulate local neuronal dendrite and synaptic spine development and contribute to disease. 2019-04-01 2019-05 /pmc/articles/PMC6551209/ /pubmed/30936556 http://dx.doi.org/10.1038/s41593-019-0366-7 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Miller, Sean J.
Philips, Thomas
Kim, Namho
Dastgheyb, Raha
Chen, Zhuoxun
Hsieh, Yi-Chun
Daigle, J. Gavin
Datta, Malika
Chew, Jeannie
Vidensky, Svetlana
Pham, Jacqueline T.
Hughes, Ethan G.
Robinson, Michael B.
Sattler, Rita
Tomer, Raju
Suk, Jung Soo
Bergles, Dwight E.
Haughey, Norman
Pletnikov, Mikhail
Hanes, Justin
Rothstein, Jeffrey D.
Molecularly defined cortical astroglia subpopulation modulates neurons via secretion of Norrin
title Molecularly defined cortical astroglia subpopulation modulates neurons via secretion of Norrin
title_full Molecularly defined cortical astroglia subpopulation modulates neurons via secretion of Norrin
title_fullStr Molecularly defined cortical astroglia subpopulation modulates neurons via secretion of Norrin
title_full_unstemmed Molecularly defined cortical astroglia subpopulation modulates neurons via secretion of Norrin
title_short Molecularly defined cortical astroglia subpopulation modulates neurons via secretion of Norrin
title_sort molecularly defined cortical astroglia subpopulation modulates neurons via secretion of norrin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6551209/
https://www.ncbi.nlm.nih.gov/pubmed/30936556
http://dx.doi.org/10.1038/s41593-019-0366-7
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