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Molecularly defined cortical astroglia subpopulation modulates neurons via secretion of Norrin
Despite expanding knowledge regarding the role of astroglia in regulating neuronal function, little is known about regional or functional subgroups of brain astroglia and how they may interact with neurons. We use an astroglia-specific promoter fragment in transgenic mice to identify an anatomically...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6551209/ https://www.ncbi.nlm.nih.gov/pubmed/30936556 http://dx.doi.org/10.1038/s41593-019-0366-7 |
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author | Miller, Sean J. Philips, Thomas Kim, Namho Dastgheyb, Raha Chen, Zhuoxun Hsieh, Yi-Chun Daigle, J. Gavin Datta, Malika Chew, Jeannie Vidensky, Svetlana Pham, Jacqueline T. Hughes, Ethan G. Robinson, Michael B. Sattler, Rita Tomer, Raju Suk, Jung Soo Bergles, Dwight E. Haughey, Norman Pletnikov, Mikhail Hanes, Justin Rothstein, Jeffrey D. |
author_facet | Miller, Sean J. Philips, Thomas Kim, Namho Dastgheyb, Raha Chen, Zhuoxun Hsieh, Yi-Chun Daigle, J. Gavin Datta, Malika Chew, Jeannie Vidensky, Svetlana Pham, Jacqueline T. Hughes, Ethan G. Robinson, Michael B. Sattler, Rita Tomer, Raju Suk, Jung Soo Bergles, Dwight E. Haughey, Norman Pletnikov, Mikhail Hanes, Justin Rothstein, Jeffrey D. |
author_sort | Miller, Sean J. |
collection | PubMed |
description | Despite expanding knowledge regarding the role of astroglia in regulating neuronal function, little is known about regional or functional subgroups of brain astroglia and how they may interact with neurons. We use an astroglia-specific promoter fragment in transgenic mice to identify an anatomically defined subset of adult grey matter astroglia. Using transcriptomic and histological analyses, we generate a combinatorial profile for in vivo identification and characterization of this astroglia subpopulation. These astroglia are enriched in cortical layer V, express distinct molecular markers, including norrin and LGR6, with corresponding layer-specific neuronal ligands, are found in human cortex and modulate neuronal activity. Astrocytic norrin appears to regulate dendrites and spines and its loss, as occurs in Norrie disease, contributes to cortical dendritic spine loss. These studies provide evidence that human and rodent astroglia subtypes are regionally and functionally distinct, can regulate local neuronal dendrite and synaptic spine development and contribute to disease. |
format | Online Article Text |
id | pubmed-6551209 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
record_format | MEDLINE/PubMed |
spelling | pubmed-65512092019-10-01 Molecularly defined cortical astroglia subpopulation modulates neurons via secretion of Norrin Miller, Sean J. Philips, Thomas Kim, Namho Dastgheyb, Raha Chen, Zhuoxun Hsieh, Yi-Chun Daigle, J. Gavin Datta, Malika Chew, Jeannie Vidensky, Svetlana Pham, Jacqueline T. Hughes, Ethan G. Robinson, Michael B. Sattler, Rita Tomer, Raju Suk, Jung Soo Bergles, Dwight E. Haughey, Norman Pletnikov, Mikhail Hanes, Justin Rothstein, Jeffrey D. Nat Neurosci Article Despite expanding knowledge regarding the role of astroglia in regulating neuronal function, little is known about regional or functional subgroups of brain astroglia and how they may interact with neurons. We use an astroglia-specific promoter fragment in transgenic mice to identify an anatomically defined subset of adult grey matter astroglia. Using transcriptomic and histological analyses, we generate a combinatorial profile for in vivo identification and characterization of this astroglia subpopulation. These astroglia are enriched in cortical layer V, express distinct molecular markers, including norrin and LGR6, with corresponding layer-specific neuronal ligands, are found in human cortex and modulate neuronal activity. Astrocytic norrin appears to regulate dendrites and spines and its loss, as occurs in Norrie disease, contributes to cortical dendritic spine loss. These studies provide evidence that human and rodent astroglia subtypes are regionally and functionally distinct, can regulate local neuronal dendrite and synaptic spine development and contribute to disease. 2019-04-01 2019-05 /pmc/articles/PMC6551209/ /pubmed/30936556 http://dx.doi.org/10.1038/s41593-019-0366-7 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Miller, Sean J. Philips, Thomas Kim, Namho Dastgheyb, Raha Chen, Zhuoxun Hsieh, Yi-Chun Daigle, J. Gavin Datta, Malika Chew, Jeannie Vidensky, Svetlana Pham, Jacqueline T. Hughes, Ethan G. Robinson, Michael B. Sattler, Rita Tomer, Raju Suk, Jung Soo Bergles, Dwight E. Haughey, Norman Pletnikov, Mikhail Hanes, Justin Rothstein, Jeffrey D. Molecularly defined cortical astroglia subpopulation modulates neurons via secretion of Norrin |
title | Molecularly defined cortical astroglia subpopulation modulates neurons via secretion of Norrin |
title_full | Molecularly defined cortical astroglia subpopulation modulates neurons via secretion of Norrin |
title_fullStr | Molecularly defined cortical astroglia subpopulation modulates neurons via secretion of Norrin |
title_full_unstemmed | Molecularly defined cortical astroglia subpopulation modulates neurons via secretion of Norrin |
title_short | Molecularly defined cortical astroglia subpopulation modulates neurons via secretion of Norrin |
title_sort | molecularly defined cortical astroglia subpopulation modulates neurons via secretion of norrin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6551209/ https://www.ncbi.nlm.nih.gov/pubmed/30936556 http://dx.doi.org/10.1038/s41593-019-0366-7 |
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