Cargando…

Enhancement of rotator cuff tendon–bone healing using bone marrow–stimulating technique along with hyaluronic acid

OBJECTIVE: The purpose of this study was to investigate the effect of hyaluronic acid (HA) in the tendon–bone healing process after rotator cuff repair in a rabbit model. METHODS: In vitro, rat bone marrow stromal cells (rBMSCs) were cultured in media for cartilage-related and inflammation-related g...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Hong, Chen, Yuzhou, Chen, Shiyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Chinese Speaking Orthopaedic Society 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6551361/
https://www.ncbi.nlm.nih.gov/pubmed/31194057
http://dx.doi.org/10.1016/j.jot.2019.01.001
_version_ 1783424370909118464
author Li, Hong
Chen, Yuzhou
Chen, Shiyi
author_facet Li, Hong
Chen, Yuzhou
Chen, Shiyi
author_sort Li, Hong
collection PubMed
description OBJECTIVE: The purpose of this study was to investigate the effect of hyaluronic acid (HA) in the tendon–bone healing process after rotator cuff repair in a rabbit model. METHODS: In vitro, rat bone marrow stromal cells (rBMSCs) were cultured in media for cartilage-related and inflammation-related gene expression levels examination at 1.0 mg/mL of HA. In vivo, 48 New Zealand white rabbits underwent rotator cuff repair surgery, and they were randomly divided into three groups: (1) control group (n = 16), (2) microfracture (MF) group accepting MF treatment (n = 16) and (3) MF/HA group accepting MF with HA treatment (n = 16). Four rabbits from each group were sacrificed at 6 and 12 weeks postoperatively for histological evaluation and biomechanical testing. RESULTS: In vitro experiments reveal that HA significantly decreased inflammation-related mRNA expression (IL-1, TNFα) compared with the control group. At 6 weeks after surgery, there was no significant difference of load-to-failure between groups. At 12 weeks after surgery, the mean failure load of the MF/HA group was significantly higher than that of the control group (100.5 ± 10.1 N vs. 68.0 ± 6.2 N; p = 0.0115). The mean failure load of the MF group appeared higher than that of the control group, whereas there was no significant difference (p > 0.05). Histologically, more chondrocytes were clustered at the tendon–bone interface, and more extracellular matrixes were produced in the MF/HA group. The interface of the MF/HA group appeared similar with the normal tendon–bone interface. CONCLUSION: HA may play a crucial role in the acceleration of tendon-to-bone healing which might be through inhibiting inflammation. Rotator cuff repair using MF along with HA led to better tendon–bone healing and a subsequent increase of biomechanical strength at the repair site. THE TRANSLATIONAL POTENTIAL OF THIS ARTICLE: HA injection is very common for patients with rotator cuff disease because of its antiinflammatory action and adhesion prevention preoperatively. The HA injection during surgery provides an antiinflammatory effect during tendon–bone healing process and leads to better tendon–bone healing postoperatively.
format Online
Article
Text
id pubmed-6551361
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Chinese Speaking Orthopaedic Society
record_format MEDLINE/PubMed
spelling pubmed-65513612019-06-10 Enhancement of rotator cuff tendon–bone healing using bone marrow–stimulating technique along with hyaluronic acid Li, Hong Chen, Yuzhou Chen, Shiyi J Orthop Translat Original Article OBJECTIVE: The purpose of this study was to investigate the effect of hyaluronic acid (HA) in the tendon–bone healing process after rotator cuff repair in a rabbit model. METHODS: In vitro, rat bone marrow stromal cells (rBMSCs) were cultured in media for cartilage-related and inflammation-related gene expression levels examination at 1.0 mg/mL of HA. In vivo, 48 New Zealand white rabbits underwent rotator cuff repair surgery, and they were randomly divided into three groups: (1) control group (n = 16), (2) microfracture (MF) group accepting MF treatment (n = 16) and (3) MF/HA group accepting MF with HA treatment (n = 16). Four rabbits from each group were sacrificed at 6 and 12 weeks postoperatively for histological evaluation and biomechanical testing. RESULTS: In vitro experiments reveal that HA significantly decreased inflammation-related mRNA expression (IL-1, TNFα) compared with the control group. At 6 weeks after surgery, there was no significant difference of load-to-failure between groups. At 12 weeks after surgery, the mean failure load of the MF/HA group was significantly higher than that of the control group (100.5 ± 10.1 N vs. 68.0 ± 6.2 N; p = 0.0115). The mean failure load of the MF group appeared higher than that of the control group, whereas there was no significant difference (p > 0.05). Histologically, more chondrocytes were clustered at the tendon–bone interface, and more extracellular matrixes were produced in the MF/HA group. The interface of the MF/HA group appeared similar with the normal tendon–bone interface. CONCLUSION: HA may play a crucial role in the acceleration of tendon-to-bone healing which might be through inhibiting inflammation. Rotator cuff repair using MF along with HA led to better tendon–bone healing and a subsequent increase of biomechanical strength at the repair site. THE TRANSLATIONAL POTENTIAL OF THIS ARTICLE: HA injection is very common for patients with rotator cuff disease because of its antiinflammatory action and adhesion prevention preoperatively. The HA injection during surgery provides an antiinflammatory effect during tendon–bone healing process and leads to better tendon–bone healing postoperatively. Chinese Speaking Orthopaedic Society 2019-01-28 /pmc/articles/PMC6551361/ /pubmed/31194057 http://dx.doi.org/10.1016/j.jot.2019.01.001 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Li, Hong
Chen, Yuzhou
Chen, Shiyi
Enhancement of rotator cuff tendon–bone healing using bone marrow–stimulating technique along with hyaluronic acid
title Enhancement of rotator cuff tendon–bone healing using bone marrow–stimulating technique along with hyaluronic acid
title_full Enhancement of rotator cuff tendon–bone healing using bone marrow–stimulating technique along with hyaluronic acid
title_fullStr Enhancement of rotator cuff tendon–bone healing using bone marrow–stimulating technique along with hyaluronic acid
title_full_unstemmed Enhancement of rotator cuff tendon–bone healing using bone marrow–stimulating technique along with hyaluronic acid
title_short Enhancement of rotator cuff tendon–bone healing using bone marrow–stimulating technique along with hyaluronic acid
title_sort enhancement of rotator cuff tendon–bone healing using bone marrow–stimulating technique along with hyaluronic acid
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6551361/
https://www.ncbi.nlm.nih.gov/pubmed/31194057
http://dx.doi.org/10.1016/j.jot.2019.01.001
work_keys_str_mv AT lihong enhancementofrotatorcufftendonbonehealingusingbonemarrowstimulatingtechniquealongwithhyaluronicacid
AT chenyuzhou enhancementofrotatorcufftendonbonehealingusingbonemarrowstimulatingtechniquealongwithhyaluronicacid
AT chenshiyi enhancementofrotatorcufftendonbonehealingusingbonemarrowstimulatingtechniquealongwithhyaluronicacid