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Acrylamide induces HepG2 cell proliferation through upregulation of miR-21 expression
Acrylamide, a potential carcinogen, exists in carbohydrate-rich foods cooked at a high temperature. It has been reported that acrylamide can cause DNA damage and cytotoxicity. The present study aimed to investigate the potential mechanism of human hepatocarcinoma HepG2 cell proliferation induced by...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Editorial Department of Journal of Biomedical Research
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6551424/ https://www.ncbi.nlm.nih.gov/pubmed/28963442 http://dx.doi.org/10.7555/JBR.31.20170016 |
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author | Xu, Yuyu Wang, Pengqi Xu, Chaoqi Shan, Xiaoyun Feng, Qing |
author_facet | Xu, Yuyu Wang, Pengqi Xu, Chaoqi Shan, Xiaoyun Feng, Qing |
author_sort | Xu, Yuyu |
collection | PubMed |
description | Acrylamide, a potential carcinogen, exists in carbohydrate-rich foods cooked at a high temperature. It has been reported that acrylamide can cause DNA damage and cytotoxicity. The present study aimed to investigate the potential mechanism of human hepatocarcinoma HepG2 cell proliferation induced by acrylamide and to explore the antagonistic effects of a natural polyphenol curcumin against acrylamide via miR-21. The results indicated that acrylamide (≤100 μmol/L) significantly increased HepG2 cell proliferation and miR-21 expression. In addition, acrylamide reduced the PTEN expression in protein level, while induced the expressions of p-AKT, EGFR and cyclin D1. The PI3K/AKT inhibitor decreased p-AKT protein expression and inhibited the proliferation of HepG2 cells. In addition, curcumin effectively reduced acrylamide-induced HepG2 cell proliferation and induced apoptosis through the expression of miR-21. In conclusion, the results showed that acrylamide increased HepG2 cell proliferation via upregulating miR-21 expression, which may be a new target for the treatment and prevention of cancer. |
format | Online Article Text |
id | pubmed-6551424 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Editorial Department of Journal of Biomedical Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-65514242019-11-20 Acrylamide induces HepG2 cell proliferation through upregulation of miR-21 expression Xu, Yuyu Wang, Pengqi Xu, Chaoqi Shan, Xiaoyun Feng, Qing J Biomed Res Original Article Acrylamide, a potential carcinogen, exists in carbohydrate-rich foods cooked at a high temperature. It has been reported that acrylamide can cause DNA damage and cytotoxicity. The present study aimed to investigate the potential mechanism of human hepatocarcinoma HepG2 cell proliferation induced by acrylamide and to explore the antagonistic effects of a natural polyphenol curcumin against acrylamide via miR-21. The results indicated that acrylamide (≤100 μmol/L) significantly increased HepG2 cell proliferation and miR-21 expression. In addition, acrylamide reduced the PTEN expression in protein level, while induced the expressions of p-AKT, EGFR and cyclin D1. The PI3K/AKT inhibitor decreased p-AKT protein expression and inhibited the proliferation of HepG2 cells. In addition, curcumin effectively reduced acrylamide-induced HepG2 cell proliferation and induced apoptosis through the expression of miR-21. In conclusion, the results showed that acrylamide increased HepG2 cell proliferation via upregulating miR-21 expression, which may be a new target for the treatment and prevention of cancer. Editorial Department of Journal of Biomedical Research 2019 2017-05-27 /pmc/articles/PMC6551424/ /pubmed/28963442 http://dx.doi.org/10.7555/JBR.31.20170016 Text en /creativecommons.org/licenses/by/4.0/ This is an open access article under the Creative Commons Attribution (CC BY 4.0) license, which permits others to distribute, remix, adapt and build upon this work, for commercial use, provided the original work is properly cited |
spellingShingle | Original Article Xu, Yuyu Wang, Pengqi Xu, Chaoqi Shan, Xiaoyun Feng, Qing Acrylamide induces HepG2 cell proliferation through upregulation of miR-21 expression |
title | Acrylamide induces HepG2 cell proliferation through upregulation of miR-21 expression |
title_full | Acrylamide induces HepG2 cell proliferation through upregulation of miR-21 expression |
title_fullStr | Acrylamide induces HepG2 cell proliferation through upregulation of miR-21 expression |
title_full_unstemmed | Acrylamide induces HepG2 cell proliferation through upregulation of miR-21 expression |
title_short | Acrylamide induces HepG2 cell proliferation through upregulation of miR-21 expression |
title_sort | acrylamide induces hepg2 cell proliferation through upregulation of mir-21 expression |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6551424/ https://www.ncbi.nlm.nih.gov/pubmed/28963442 http://dx.doi.org/10.7555/JBR.31.20170016 |
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