SAT-047 Disorders in Mice with Subclinical Hypothyroidism

Subclinical hypothyroidism (SCH) is becoming a global health problem because of its increasing prevalence and potential adverse effects, including cardiovascular diseases and nonalcoholic fatty liver disease. However, the association remains controversial. MicroRNAs (miRNAs) have been shown to be implicated in lipid metabolism disorders. But how miRNAs regulate hepatic lipid metabolism in SCH remains unknown. We investigated miRNA alterations and proteome profiles in a SCH mouse model, which were generated with methimazole(MMI) for 16 weeks. The profiles of 17 miRNAs which are critical to hepatic lipid metabolism and proteome were investigated using quantitative PCR and iTRAQ labeling in the livers from SCH (n=9) and control mice (CON, n=7). Putative targets prediction of miRNAs was carried out using Targetscan and miRanda. Compared with the control mice, SCH mice had significantly different expressed 8 miRNAs and 36 proteins in livers. We identified a regulatory module containing 3 miRNAs (miR-34a-5p, miR-24-3p, miR-130a-3p ) and 5 proteins (Thioredoxin (Txn), Aldehyde dehydrogenase (Aldh3a2), Selenium-binding protein 2(Selenbp2), Elongation factor 1-beta( Eef1b) and Prosaposin, isoform CRA-a (Psap)). Integrated analysis of miRNA and proteome highlighted a regulatory module between miRNAs and proteins, which, to some extent, may contribute to a better understanding of hepatic lipid metabolism disorders in SCH mice.