SUN-036 SLC16A11 Gene Expression In Mothers With And Without Gestational Diabetes And Their Offspring Umbilical Cord Blood.

BACKGROUND: Studies have identified the intrauterine environment as a regulating factor of the methylation profiles of DNA of newborns. In Mexicans a SLC16A11 risk haplotypes is associated with type 2 diabetes mellitus. It is in our interest to evaluate SLC16A11 expression from the earliest stages of human development in the cells of umbilical cord blood of newborn offspring of mothers with and without gestational diabetes mellitus (GDM), and thus identify indirectly epigenetic modifications due to maternal hyperglycemic status. To date is scarce this kind of studies. OBJECTIVE: To determine the SLC16A11 gene expression, in the cells of umbilical cord blood of newborn offspring of mothers with or without GDM. MATERIAL AND METHODS: Pregnant patients submitting to cesarean, with or without GDM, were invited to participate in at Gyneco-obstetric hospital of IMSS, Mexico City. RNA of peripheral blood cells (PBC) from mothers with or without GDM, and from the umbilical cord blood cells of their newborn offspring, were extracted. Briefly, to evaluate differences in SLC16A11 gene expression, cDNA was generated by using retro-transcriptase reaction and then subjected to end point PCR with specific oligonucleotides. Variables were analyzed with t-Student test using the SPSS program. Values of p < 0.05 were considered statistically significant. RESULTS: Mean age of mothers was 30 ± 4.25 years. GDM mothers were heavier (32.90 ± 3.7 vs 30.12 ± 3.4 kg/m(2), p=0.032), but there were no differences in glycemic control (HbA1c)(5.4 ± 0.6 vs 5.2 ± 0.6, p=0.333). Somatometric measurements of offspring showed no significant differences (weight: 3013.33 ± 675.54 vs 3135.71g ± 740.59, p=0.626 and length: 48.50 ± 3.03 vs 49.17cm ± 1.56, p=0.368). Analysis SLC16A11 gene expression did not showed differences in mothers, (p=0.059), and in offspring (p=0.315). Because there is a SLC16A11 gene expression, this could suggest that the SLC16A11 gene promoter could not be methylated. CONCLUSIONS: No modifications in SLC16A11 gene expression were present in GDM, which suggests that GDM does not modify the molecular mechanism in SLC16A11 RNA expression.