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ZnO-NPs embedded biodegradable thiolated bandage for postoperative surgical site infection: In vitro and in vivo evaluation
Post-operative surgical site infections (SSI) present a serious threat and may lead to complications. Currently available dressings for SSI lack mucoadhesion, safety, efficacy and most importantly patient compliance. We aimed to address these concerns by developing a bioactive thiolated chitosan-alg...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6553718/ https://www.ncbi.nlm.nih.gov/pubmed/31170179 http://dx.doi.org/10.1371/journal.pone.0217079 |
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author | Arshad, Rabia Sohail, Muhammad Farhan Sarwar, Hafiz Shoaib Saeed, Hamid Ali, Imran Akhtar, Sohail Hussain, Syed Zajif Afzal, Iqra Jahan, Sarwat Anees-ur-Rehman, Shahnaz, Gul |
author_facet | Arshad, Rabia Sohail, Muhammad Farhan Sarwar, Hafiz Shoaib Saeed, Hamid Ali, Imran Akhtar, Sohail Hussain, Syed Zajif Afzal, Iqra Jahan, Sarwat Anees-ur-Rehman, Shahnaz, Gul |
author_sort | Arshad, Rabia |
collection | PubMed |
description | Post-operative surgical site infections (SSI) present a serious threat and may lead to complications. Currently available dressings for SSI lack mucoadhesion, safety, efficacy and most importantly patient compliance. We aimed to address these concerns by developing a bioactive thiolated chitosan-alginate bandage embedded with zinc oxide nanoparticles (ZnO-NPs) for localized topical treatment of SSI. The FTIR, XRD, DSC and TGA of bandage confirmed the compatibility of ingredients and modifications made. The porosity, swelling index and lysozyme degradation showed good properties for wound healing and biodegradation. Moreover, in-vitro antibacterial activity showed higher bactericidal effect as compared to ZnO-NPs free bandage. In-vivo wound healing in murine model showed significant improved tissue generation and speedy wound healing as compared to positive and negative controls. Over all, thiolated bandage showed potential as an advanced therapeutic agent for treating surgical site infections, meeting the required features of an ideal surgical dressing. |
format | Online Article Text |
id | pubmed-6553718 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-65537182019-06-17 ZnO-NPs embedded biodegradable thiolated bandage for postoperative surgical site infection: In vitro and in vivo evaluation Arshad, Rabia Sohail, Muhammad Farhan Sarwar, Hafiz Shoaib Saeed, Hamid Ali, Imran Akhtar, Sohail Hussain, Syed Zajif Afzal, Iqra Jahan, Sarwat Anees-ur-Rehman, Shahnaz, Gul PLoS One Research Article Post-operative surgical site infections (SSI) present a serious threat and may lead to complications. Currently available dressings for SSI lack mucoadhesion, safety, efficacy and most importantly patient compliance. We aimed to address these concerns by developing a bioactive thiolated chitosan-alginate bandage embedded with zinc oxide nanoparticles (ZnO-NPs) for localized topical treatment of SSI. The FTIR, XRD, DSC and TGA of bandage confirmed the compatibility of ingredients and modifications made. The porosity, swelling index and lysozyme degradation showed good properties for wound healing and biodegradation. Moreover, in-vitro antibacterial activity showed higher bactericidal effect as compared to ZnO-NPs free bandage. In-vivo wound healing in murine model showed significant improved tissue generation and speedy wound healing as compared to positive and negative controls. Over all, thiolated bandage showed potential as an advanced therapeutic agent for treating surgical site infections, meeting the required features of an ideal surgical dressing. Public Library of Science 2019-06-06 /pmc/articles/PMC6553718/ /pubmed/31170179 http://dx.doi.org/10.1371/journal.pone.0217079 Text en © 2019 Arshad et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Arshad, Rabia Sohail, Muhammad Farhan Sarwar, Hafiz Shoaib Saeed, Hamid Ali, Imran Akhtar, Sohail Hussain, Syed Zajif Afzal, Iqra Jahan, Sarwat Anees-ur-Rehman, Shahnaz, Gul ZnO-NPs embedded biodegradable thiolated bandage for postoperative surgical site infection: In vitro and in vivo evaluation |
title | ZnO-NPs embedded biodegradable thiolated bandage for postoperative surgical site infection: In vitro and in vivo evaluation |
title_full | ZnO-NPs embedded biodegradable thiolated bandage for postoperative surgical site infection: In vitro and in vivo evaluation |
title_fullStr | ZnO-NPs embedded biodegradable thiolated bandage for postoperative surgical site infection: In vitro and in vivo evaluation |
title_full_unstemmed | ZnO-NPs embedded biodegradable thiolated bandage for postoperative surgical site infection: In vitro and in vivo evaluation |
title_short | ZnO-NPs embedded biodegradable thiolated bandage for postoperative surgical site infection: In vitro and in vivo evaluation |
title_sort | zno-nps embedded biodegradable thiolated bandage for postoperative surgical site infection: in vitro and in vivo evaluation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6553718/ https://www.ncbi.nlm.nih.gov/pubmed/31170179 http://dx.doi.org/10.1371/journal.pone.0217079 |
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