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Identifying parameters to improve the reproducibility of transient gene expression in High Five cells

Virus-free, transient gene expression (TGE) in High Five cells was recently presented as an efficient protein production method. However, published TGE protocols have not been standardized to a general protocol. Therefore, reproducibility and implementation of the method in other labs remains diffic...

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Detalles Bibliográficos
Autores principales: Bleckmann, Maren, Schürig, Margitta, Endres, Michelle, Samuels, Anke, Gebauer, Daniela, Konisch, Nadine, van den Heuvel, Joop
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6553862/
https://www.ncbi.nlm.nih.gov/pubmed/31170233
http://dx.doi.org/10.1371/journal.pone.0217878