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Optimization and Comparison of Different Methods and Factors for Efficient Transformation of Brucella abortus RB51strain

BACKGROUND: The development of protective vaccines for Brucella spp. has been hampered by the difficulty in transformation of Brucella cells with foreign DNA for genetic manipulation. It seems that the formation of Brucella spheroplasts would increase the efficiency of transformation. The aim of thi...

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Detalles Bibliográficos
Autores principales:	Gheibi, Azam, Khanahmad, Hossein, Kardar, Gholam Ali, Boshtam, Maryam, Rezaie, Sassan, Kazemi, Bahram, Khorramizadeh, Mohammad Reza
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Wolters Kluwer - Medknow 2019
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6555225/ https://www.ncbi.nlm.nih.gov/pubmed/31198771 http://dx.doi.org/10.4103/abr.abr_14_19

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