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Ultrastructural Changes of Collagen in Different Histopathological Grades of Oral Submucous Fibrosis

BACKGROUND AND OBJECTIVES: Oral submucous fibrosis (OSMF) is a potentially malignant disorder and a crippling condition of oral mucosa. It is usually seen in adults with areca nut chewing habit, which is characterized by changes in the connective tissue fibers leading to stiffness of the mucosa and...

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Autores principales: Tom, Arun, Baghirath, Venkat, Krishna, Bhargavi, Ganepalli, Ashalata, Kumar, Jogishetty Vijay, Mohan, Sunil Paramel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6555333/
https://www.ncbi.nlm.nih.gov/pubmed/31198360
http://dx.doi.org/10.4103/JPBS.JPBS_20_19
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author Tom, Arun
Baghirath, Venkat
Krishna, Bhargavi
Ganepalli, Ashalata
Kumar, Jogishetty Vijay
Mohan, Sunil Paramel
author_facet Tom, Arun
Baghirath, Venkat
Krishna, Bhargavi
Ganepalli, Ashalata
Kumar, Jogishetty Vijay
Mohan, Sunil Paramel
author_sort Tom, Arun
collection PubMed
description BACKGROUND AND OBJECTIVES: Oral submucous fibrosis (OSMF) is a potentially malignant disorder and a crippling condition of oral mucosa. It is usually seen in adults with areca nut chewing habit, which is characterized by changes in the connective tissue fibers leading to stiffness of the mucosa and restricted mouth opening. Patients with severe cases have distinct difficulties in chewing, swallowing, and speaking. It predominantly occurs in Indians and other population of the Indian subcontinent with a prevalence of 0.2%–0.5%. The potentiality of malignant transformation associated with OSMF is much higher with a range of 4.5%–7.6%. This article is about the ultrastructural changes pertaining to collagen and with respect to the histopathological grades of OSMF. MATERIALS AND METHODS: A total of 20 biopsy samples from clinically diagnosed OSMF subjects and 5 healthy controls were collected. After obtaining the biopsy, the specimens were divided into two halves, of which one was studied under light microscope and the other half was observed under transmission electron microscope. RESULTS: Of the 20 subjects in the study group, most of the subjects were in the 20–29 years age group with an overall male predilection (19 subjects). Four subjects were in grade 1, nine were in grade 2, and seven were in grade 3. Collagen showed sparse to dense fibrosis and normal to thick collagen bundles, and some subjects showed encroachment of collagen into the blood vessels. CONCLUSION: This study showed definitive changes with respect to collagen in the OSMF samples compared to the controls. The changes were found to be increasing with the progression of the disease to the higher grades. The changes were pertaining to the collagen and were drawing toward an increased fibrosis of the connective tissue, which compresses the blood vessels. Hence, the state of hypoperfusion and subsequent epithelial atrophy can be considered in the progression of disease to the higher grades.
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spelling pubmed-65553332019-06-13 Ultrastructural Changes of Collagen in Different Histopathological Grades of Oral Submucous Fibrosis Tom, Arun Baghirath, Venkat Krishna, Bhargavi Ganepalli, Ashalata Kumar, Jogishetty Vijay Mohan, Sunil Paramel J Pharm Bioallied Sci Original Article BACKGROUND AND OBJECTIVES: Oral submucous fibrosis (OSMF) is a potentially malignant disorder and a crippling condition of oral mucosa. It is usually seen in adults with areca nut chewing habit, which is characterized by changes in the connective tissue fibers leading to stiffness of the mucosa and restricted mouth opening. Patients with severe cases have distinct difficulties in chewing, swallowing, and speaking. It predominantly occurs in Indians and other population of the Indian subcontinent with a prevalence of 0.2%–0.5%. The potentiality of malignant transformation associated with OSMF is much higher with a range of 4.5%–7.6%. This article is about the ultrastructural changes pertaining to collagen and with respect to the histopathological grades of OSMF. MATERIALS AND METHODS: A total of 20 biopsy samples from clinically diagnosed OSMF subjects and 5 healthy controls were collected. After obtaining the biopsy, the specimens were divided into two halves, of which one was studied under light microscope and the other half was observed under transmission electron microscope. RESULTS: Of the 20 subjects in the study group, most of the subjects were in the 20–29 years age group with an overall male predilection (19 subjects). Four subjects were in grade 1, nine were in grade 2, and seven were in grade 3. Collagen showed sparse to dense fibrosis and normal to thick collagen bundles, and some subjects showed encroachment of collagen into the blood vessels. CONCLUSION: This study showed definitive changes with respect to collagen in the OSMF samples compared to the controls. The changes were found to be increasing with the progression of the disease to the higher grades. The changes were pertaining to the collagen and were drawing toward an increased fibrosis of the connective tissue, which compresses the blood vessels. Hence, the state of hypoperfusion and subsequent epithelial atrophy can be considered in the progression of disease to the higher grades. Medknow Publications & Media Pvt Ltd 2019-05 /pmc/articles/PMC6555333/ /pubmed/31198360 http://dx.doi.org/10.4103/JPBS.JPBS_20_19 Text en Copyright: © 2019 Journal of Pharmacy and Bioallied Sciences http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Tom, Arun
Baghirath, Venkat
Krishna, Bhargavi
Ganepalli, Ashalata
Kumar, Jogishetty Vijay
Mohan, Sunil Paramel
Ultrastructural Changes of Collagen in Different Histopathological Grades of Oral Submucous Fibrosis
title Ultrastructural Changes of Collagen in Different Histopathological Grades of Oral Submucous Fibrosis
title_full Ultrastructural Changes of Collagen in Different Histopathological Grades of Oral Submucous Fibrosis
title_fullStr Ultrastructural Changes of Collagen in Different Histopathological Grades of Oral Submucous Fibrosis
title_full_unstemmed Ultrastructural Changes of Collagen in Different Histopathological Grades of Oral Submucous Fibrosis
title_short Ultrastructural Changes of Collagen in Different Histopathological Grades of Oral Submucous Fibrosis
title_sort ultrastructural changes of collagen in different histopathological grades of oral submucous fibrosis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6555333/
https://www.ncbi.nlm.nih.gov/pubmed/31198360
http://dx.doi.org/10.4103/JPBS.JPBS_20_19
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