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Kerosene as an Alternative to Xylene in Histopathological Tissue Processing and Staining: An Experimental Study

BACKGROUND: Conventional tissue processing is as old as 100 years and still remains the gold standard. Tissue processing involves many steps, of which one of the important steps is clearing. Xylene is one of the common clearing agents used in laboratory, but it is carcinogenic and teratogenic. AIM:...

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Autores principales: Dineshshankar, Janardhanam, Saranya, Manikandan, Tamilthangam, Periyasamy, Swathiraman, Jeyaraman, Shanmathee, Kumaravel, Preethi, Ravichandran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6555370/
https://www.ncbi.nlm.nih.gov/pubmed/31198372
http://dx.doi.org/10.4103/JPBS.JPBS_38_19
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author Dineshshankar, Janardhanam
Saranya, Manikandan
Tamilthangam, Periyasamy
Swathiraman, Jeyaraman
Shanmathee, Kumaravel
Preethi, Ravichandran
author_facet Dineshshankar, Janardhanam
Saranya, Manikandan
Tamilthangam, Periyasamy
Swathiraman, Jeyaraman
Shanmathee, Kumaravel
Preethi, Ravichandran
author_sort Dineshshankar, Janardhanam
collection PubMed
description BACKGROUND: Conventional tissue processing is as old as 100 years and still remains the gold standard. Tissue processing involves many steps, of which one of the important steps is clearing. Xylene is one of the common clearing agents used in laboratory, but it is carcinogenic and teratogenic. AIM: The aim of this study was to substitute conventionally used xylene with kerosene in tissue processing and staining. MATERIALS AND METHODS: Thirty bits of chicken tissue samples were collected; each was randomly separated into two groups: tissue processing and staining. Instead of conventional xylene, we used kerosene. The tissue blocks were subjected to sectioning and staining, and finally, they were observed under light microscope. RESULTS: Tissue samples that were processed and cleared with kerosene showed equal clearing and staining without any alterations of the tissue morphology and cellular details with that of xylene. CONCLUSION: Kerosene can be used as a substitute to xylene without posing any health risk or compromising the cellular integrity.
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spelling pubmed-65553702019-06-13 Kerosene as an Alternative to Xylene in Histopathological Tissue Processing and Staining: An Experimental Study Dineshshankar, Janardhanam Saranya, Manikandan Tamilthangam, Periyasamy Swathiraman, Jeyaraman Shanmathee, Kumaravel Preethi, Ravichandran J Pharm Bioallied Sci Original Article BACKGROUND: Conventional tissue processing is as old as 100 years and still remains the gold standard. Tissue processing involves many steps, of which one of the important steps is clearing. Xylene is one of the common clearing agents used in laboratory, but it is carcinogenic and teratogenic. AIM: The aim of this study was to substitute conventionally used xylene with kerosene in tissue processing and staining. MATERIALS AND METHODS: Thirty bits of chicken tissue samples were collected; each was randomly separated into two groups: tissue processing and staining. Instead of conventional xylene, we used kerosene. The tissue blocks were subjected to sectioning and staining, and finally, they were observed under light microscope. RESULTS: Tissue samples that were processed and cleared with kerosene showed equal clearing and staining without any alterations of the tissue morphology and cellular details with that of xylene. CONCLUSION: Kerosene can be used as a substitute to xylene without posing any health risk or compromising the cellular integrity. Medknow Publications & Media Pvt Ltd 2019-05 /pmc/articles/PMC6555370/ /pubmed/31198372 http://dx.doi.org/10.4103/JPBS.JPBS_38_19 Text en Copyright: © 2019 Journal of Pharmacy and Bioallied Sciences http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Dineshshankar, Janardhanam
Saranya, Manikandan
Tamilthangam, Periyasamy
Swathiraman, Jeyaraman
Shanmathee, Kumaravel
Preethi, Ravichandran
Kerosene as an Alternative to Xylene in Histopathological Tissue Processing and Staining: An Experimental Study
title Kerosene as an Alternative to Xylene in Histopathological Tissue Processing and Staining: An Experimental Study
title_full Kerosene as an Alternative to Xylene in Histopathological Tissue Processing and Staining: An Experimental Study
title_fullStr Kerosene as an Alternative to Xylene in Histopathological Tissue Processing and Staining: An Experimental Study
title_full_unstemmed Kerosene as an Alternative to Xylene in Histopathological Tissue Processing and Staining: An Experimental Study
title_short Kerosene as an Alternative to Xylene in Histopathological Tissue Processing and Staining: An Experimental Study
title_sort kerosene as an alternative to xylene in histopathological tissue processing and staining: an experimental study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6555370/
https://www.ncbi.nlm.nih.gov/pubmed/31198372
http://dx.doi.org/10.4103/JPBS.JPBS_38_19
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