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Full-field structured-illumination super-resolution X-ray transmission microscopy
Modern transmission X-ray microscopy techniques provide very high resolution at low and medium X-ray energies, but suffer from a limited field-of-view. If sub-micrometre resolution is desired, their field-of-view is typically limited to less than one millimetre. Although the field-of-view increases...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6555788/ https://www.ncbi.nlm.nih.gov/pubmed/31175291 http://dx.doi.org/10.1038/s41467-019-10537-x |
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author | Günther, Benedikt Hehn, Lorenz Jud, Christoph Hipp, Alexander Dierolf, Martin Pfeiffer, Franz |
author_facet | Günther, Benedikt Hehn, Lorenz Jud, Christoph Hipp, Alexander Dierolf, Martin Pfeiffer, Franz |
author_sort | Günther, Benedikt |
collection | PubMed |
description | Modern transmission X-ray microscopy techniques provide very high resolution at low and medium X-ray energies, but suffer from a limited field-of-view. If sub-micrometre resolution is desired, their field-of-view is typically limited to less than one millimetre. Although the field-of-view increases through combining multiple images from adjacent regions of the specimen, so does the required data acquisition time. Here, we present a method for fast full-field super-resolution transmission microscopy by structured illumination of the specimen. This technique is well-suited even for hard X-ray energies above 30 keV, where efficient optics are hard to obtain. Accordingly, investigation of optically thick specimen becomes possible with our method combining a wide field-of-view spanning multiple millimetres, or even centimetres, with sub-micron resolution and hard X-ray energies. |
format | Online Article Text |
id | pubmed-6555788 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-65557882019-06-21 Full-field structured-illumination super-resolution X-ray transmission microscopy Günther, Benedikt Hehn, Lorenz Jud, Christoph Hipp, Alexander Dierolf, Martin Pfeiffer, Franz Nat Commun Article Modern transmission X-ray microscopy techniques provide very high resolution at low and medium X-ray energies, but suffer from a limited field-of-view. If sub-micrometre resolution is desired, their field-of-view is typically limited to less than one millimetre. Although the field-of-view increases through combining multiple images from adjacent regions of the specimen, so does the required data acquisition time. Here, we present a method for fast full-field super-resolution transmission microscopy by structured illumination of the specimen. This technique is well-suited even for hard X-ray energies above 30 keV, where efficient optics are hard to obtain. Accordingly, investigation of optically thick specimen becomes possible with our method combining a wide field-of-view spanning multiple millimetres, or even centimetres, with sub-micron resolution and hard X-ray energies. Nature Publishing Group UK 2019-06-07 /pmc/articles/PMC6555788/ /pubmed/31175291 http://dx.doi.org/10.1038/s41467-019-10537-x Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Günther, Benedikt Hehn, Lorenz Jud, Christoph Hipp, Alexander Dierolf, Martin Pfeiffer, Franz Full-field structured-illumination super-resolution X-ray transmission microscopy |
title | Full-field structured-illumination super-resolution X-ray transmission microscopy |
title_full | Full-field structured-illumination super-resolution X-ray transmission microscopy |
title_fullStr | Full-field structured-illumination super-resolution X-ray transmission microscopy |
title_full_unstemmed | Full-field structured-illumination super-resolution X-ray transmission microscopy |
title_short | Full-field structured-illumination super-resolution X-ray transmission microscopy |
title_sort | full-field structured-illumination super-resolution x-ray transmission microscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6555788/ https://www.ncbi.nlm.nih.gov/pubmed/31175291 http://dx.doi.org/10.1038/s41467-019-10537-x |
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