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Full-field structured-illumination super-resolution X-ray transmission microscopy

Modern transmission X-ray microscopy techniques provide very high resolution at low and medium X-ray energies, but suffer from a limited field-of-view. If sub-micrometre resolution is desired, their field-of-view is typically limited to less than one millimetre. Although the field-of-view increases...

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Autores principales: Günther, Benedikt, Hehn, Lorenz, Jud, Christoph, Hipp, Alexander, Dierolf, Martin, Pfeiffer, Franz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6555788/
https://www.ncbi.nlm.nih.gov/pubmed/31175291
http://dx.doi.org/10.1038/s41467-019-10537-x
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author Günther, Benedikt
Hehn, Lorenz
Jud, Christoph
Hipp, Alexander
Dierolf, Martin
Pfeiffer, Franz
author_facet Günther, Benedikt
Hehn, Lorenz
Jud, Christoph
Hipp, Alexander
Dierolf, Martin
Pfeiffer, Franz
author_sort Günther, Benedikt
collection PubMed
description Modern transmission X-ray microscopy techniques provide very high resolution at low and medium X-ray energies, but suffer from a limited field-of-view. If sub-micrometre resolution is desired, their field-of-view is typically limited to less than one millimetre. Although the field-of-view increases through combining multiple images from adjacent regions of the specimen, so does the required data acquisition time. Here, we present a method for fast full-field super-resolution transmission microscopy by structured illumination of the specimen. This technique is well-suited even for hard X-ray energies above 30 keV, where efficient optics are hard to obtain. Accordingly, investigation of optically thick specimen becomes possible with our method combining a wide field-of-view spanning multiple millimetres, or even centimetres, with sub-micron resolution and hard X-ray energies.
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spelling pubmed-65557882019-06-21 Full-field structured-illumination super-resolution X-ray transmission microscopy Günther, Benedikt Hehn, Lorenz Jud, Christoph Hipp, Alexander Dierolf, Martin Pfeiffer, Franz Nat Commun Article Modern transmission X-ray microscopy techniques provide very high resolution at low and medium X-ray energies, but suffer from a limited field-of-view. If sub-micrometre resolution is desired, their field-of-view is typically limited to less than one millimetre. Although the field-of-view increases through combining multiple images from adjacent regions of the specimen, so does the required data acquisition time. Here, we present a method for fast full-field super-resolution transmission microscopy by structured illumination of the specimen. This technique is well-suited even for hard X-ray energies above 30 keV, where efficient optics are hard to obtain. Accordingly, investigation of optically thick specimen becomes possible with our method combining a wide field-of-view spanning multiple millimetres, or even centimetres, with sub-micron resolution and hard X-ray energies. Nature Publishing Group UK 2019-06-07 /pmc/articles/PMC6555788/ /pubmed/31175291 http://dx.doi.org/10.1038/s41467-019-10537-x Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Günther, Benedikt
Hehn, Lorenz
Jud, Christoph
Hipp, Alexander
Dierolf, Martin
Pfeiffer, Franz
Full-field structured-illumination super-resolution X-ray transmission microscopy
title Full-field structured-illumination super-resolution X-ray transmission microscopy
title_full Full-field structured-illumination super-resolution X-ray transmission microscopy
title_fullStr Full-field structured-illumination super-resolution X-ray transmission microscopy
title_full_unstemmed Full-field structured-illumination super-resolution X-ray transmission microscopy
title_short Full-field structured-illumination super-resolution X-ray transmission microscopy
title_sort full-field structured-illumination super-resolution x-ray transmission microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6555788/
https://www.ncbi.nlm.nih.gov/pubmed/31175291
http://dx.doi.org/10.1038/s41467-019-10537-x
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