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Investigation of cell culture conditions for optimal foot-and-mouth disease virus production

BACKGROUND: Foot-and-mouth disease is a highly contagious and economically devastating disease with endemic occurrence in many parts of the world. Vaccination is the method of choice to eradicate the disease and to limit the viral spread. The vaccine production process is based on mammalian cell cul...

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Autores principales: Dill, Veronika, Zimmer, Aline, Beer, Martin, Eschbaumer, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6555971/
https://www.ncbi.nlm.nih.gov/pubmed/31174517
http://dx.doi.org/10.1186/s12896-019-0527-5
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author Dill, Veronika
Zimmer, Aline
Beer, Martin
Eschbaumer, Michael
author_facet Dill, Veronika
Zimmer, Aline
Beer, Martin
Eschbaumer, Michael
author_sort Dill, Veronika
collection PubMed
description BACKGROUND: Foot-and-mouth disease is a highly contagious and economically devastating disease with endemic occurrence in many parts of the world. Vaccination is the method of choice to eradicate the disease and to limit the viral spread. The vaccine production process is based on mammalian cell culture, in which the viral yield varies in dependence of the composition of the culture media. For foot-and-mouth disease virus (FMDV), very little is known about the culture media components that are necessary to grow the virus to high titers in cell culture. RESULTS: This study examined the influence of increasing concentrations of glucose, glutamine, ammonium chloride and different cell densities on the yield of FMDV. While an excess of glucose or glutamine does not affect the viral yield, increasing cell density reduces the viral titer by a log(10) step at a cell density of 3 × 10(6) cells/mL. This can be mitigated by performing a 100% media exchange before infection of the cells. CONCLUSIONS: The reasons for the diminished viral growth, if no complete media exchange has been performed prior to infection, remain unclear and further studies are necessary to investigate the causes more deeply. For now, the results argue for a vaccine production process with 100% media exchange to reliably obtain high viral titers. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12896-019-0527-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-65559712019-06-10 Investigation of cell culture conditions for optimal foot-and-mouth disease virus production Dill, Veronika Zimmer, Aline Beer, Martin Eschbaumer, Michael BMC Biotechnol Research Article BACKGROUND: Foot-and-mouth disease is a highly contagious and economically devastating disease with endemic occurrence in many parts of the world. Vaccination is the method of choice to eradicate the disease and to limit the viral spread. The vaccine production process is based on mammalian cell culture, in which the viral yield varies in dependence of the composition of the culture media. For foot-and-mouth disease virus (FMDV), very little is known about the culture media components that are necessary to grow the virus to high titers in cell culture. RESULTS: This study examined the influence of increasing concentrations of glucose, glutamine, ammonium chloride and different cell densities on the yield of FMDV. While an excess of glucose or glutamine does not affect the viral yield, increasing cell density reduces the viral titer by a log(10) step at a cell density of 3 × 10(6) cells/mL. This can be mitigated by performing a 100% media exchange before infection of the cells. CONCLUSIONS: The reasons for the diminished viral growth, if no complete media exchange has been performed prior to infection, remain unclear and further studies are necessary to investigate the causes more deeply. For now, the results argue for a vaccine production process with 100% media exchange to reliably obtain high viral titers. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12896-019-0527-5) contains supplementary material, which is available to authorized users. BioMed Central 2019-06-07 /pmc/articles/PMC6555971/ /pubmed/31174517 http://dx.doi.org/10.1186/s12896-019-0527-5 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Dill, Veronika
Zimmer, Aline
Beer, Martin
Eschbaumer, Michael
Investigation of cell culture conditions for optimal foot-and-mouth disease virus production
title Investigation of cell culture conditions for optimal foot-and-mouth disease virus production
title_full Investigation of cell culture conditions for optimal foot-and-mouth disease virus production
title_fullStr Investigation of cell culture conditions for optimal foot-and-mouth disease virus production
title_full_unstemmed Investigation of cell culture conditions for optimal foot-and-mouth disease virus production
title_short Investigation of cell culture conditions for optimal foot-and-mouth disease virus production
title_sort investigation of cell culture conditions for optimal foot-and-mouth disease virus production
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6555971/
https://www.ncbi.nlm.nih.gov/pubmed/31174517
http://dx.doi.org/10.1186/s12896-019-0527-5
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