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The role of microRNA-30a and downstream snail1 on the growth and metastasis of melanoma tumor

OBJECTIVE(S): Growing evidences have indicated microRNAs as modulators of tumor development and aggression. On the other hand, a phenomenon known as epithelial-mesenchymal transition (EMT) that indicates a transient phase from epithelial-like features to mesenchymal phenotype is a key player in tumo...

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Autores principales: Noori, Jahangir, Haghjooy Javanmard, Shaghayegh, Sharifi, Mohamadreza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6556508/
https://www.ncbi.nlm.nih.gov/pubmed/31217934
http://dx.doi.org/10.22038/IJBMS.2019.32317.7745
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author Noori, Jahangir
Haghjooy Javanmard, Shaghayegh
Sharifi, Mohamadreza
author_facet Noori, Jahangir
Haghjooy Javanmard, Shaghayegh
Sharifi, Mohamadreza
author_sort Noori, Jahangir
collection PubMed
description OBJECTIVE(S): Growing evidences have indicated microRNAs as modulators of tumor development and aggression. On the other hand, a phenomenon known as epithelial-mesenchymal transition (EMT) that indicates a transient phase from epithelial-like features to mesenchymal phenotype is a key player in tumor progression. In this study, we aimed to assess the potential impacts of miR-30a-5p as an inhibitor of melanoma progression and metastasis. MATERIALS AND METHODS: MiR-30a-5p was transfected into B16-F10 melanoma cells. Then, the B16-F10 cells were injected subcutaneously or intravenously (IV) in to C57BL/6 mice. Then, the mice were euthanized and tumor size, tumor weight, snail1 protein expression and nodules in the lungs were evaluated. RESULTS: The migration of cancerous cells was significantly suppressed in vitro following the ectopic presentation of miR-30a-5p into B16-F10 melanoma cells. Furthermore, the metastatic behavior of the neoplastic cells was further suppressed in a xenograft mouse model of melanoma as observed with limited lung infiltration. We also found that transfected miR-30a-5p into melanoma cells could decrease snail1 and N-cadherin expression. CONCLUSION: MiR-30a-5p may represent an effective therapeutic target for the management of melanoma and other snail-overexpressing neoplasms.
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spelling pubmed-65565082019-06-19 The role of microRNA-30a and downstream snail1 on the growth and metastasis of melanoma tumor Noori, Jahangir Haghjooy Javanmard, Shaghayegh Sharifi, Mohamadreza Iran J Basic Med Sci Original Article OBJECTIVE(S): Growing evidences have indicated microRNAs as modulators of tumor development and aggression. On the other hand, a phenomenon known as epithelial-mesenchymal transition (EMT) that indicates a transient phase from epithelial-like features to mesenchymal phenotype is a key player in tumor progression. In this study, we aimed to assess the potential impacts of miR-30a-5p as an inhibitor of melanoma progression and metastasis. MATERIALS AND METHODS: MiR-30a-5p was transfected into B16-F10 melanoma cells. Then, the B16-F10 cells were injected subcutaneously or intravenously (IV) in to C57BL/6 mice. Then, the mice were euthanized and tumor size, tumor weight, snail1 protein expression and nodules in the lungs were evaluated. RESULTS: The migration of cancerous cells was significantly suppressed in vitro following the ectopic presentation of miR-30a-5p into B16-F10 melanoma cells. Furthermore, the metastatic behavior of the neoplastic cells was further suppressed in a xenograft mouse model of melanoma as observed with limited lung infiltration. We also found that transfected miR-30a-5p into melanoma cells could decrease snail1 and N-cadherin expression. CONCLUSION: MiR-30a-5p may represent an effective therapeutic target for the management of melanoma and other snail-overexpressing neoplasms. Mashhad University of Medical Sciences 2019-05 /pmc/articles/PMC6556508/ /pubmed/31217934 http://dx.doi.org/10.22038/IJBMS.2019.32317.7745 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Noori, Jahangir
Haghjooy Javanmard, Shaghayegh
Sharifi, Mohamadreza
The role of microRNA-30a and downstream snail1 on the growth and metastasis of melanoma tumor
title The role of microRNA-30a and downstream snail1 on the growth and metastasis of melanoma tumor
title_full The role of microRNA-30a and downstream snail1 on the growth and metastasis of melanoma tumor
title_fullStr The role of microRNA-30a and downstream snail1 on the growth and metastasis of melanoma tumor
title_full_unstemmed The role of microRNA-30a and downstream snail1 on the growth and metastasis of melanoma tumor
title_short The role of microRNA-30a and downstream snail1 on the growth and metastasis of melanoma tumor
title_sort role of microrna-30a and downstream snail1 on the growth and metastasis of melanoma tumor
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6556508/
https://www.ncbi.nlm.nih.gov/pubmed/31217934
http://dx.doi.org/10.22038/IJBMS.2019.32317.7745
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