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The role of microRNA-30a and downstream snail1 on the growth and metastasis of melanoma tumor
OBJECTIVE(S): Growing evidences have indicated microRNAs as modulators of tumor development and aggression. On the other hand, a phenomenon known as epithelial-mesenchymal transition (EMT) that indicates a transient phase from epithelial-like features to mesenchymal phenotype is a key player in tumo...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6556508/ https://www.ncbi.nlm.nih.gov/pubmed/31217934 http://dx.doi.org/10.22038/IJBMS.2019.32317.7745 |
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author | Noori, Jahangir Haghjooy Javanmard, Shaghayegh Sharifi, Mohamadreza |
author_facet | Noori, Jahangir Haghjooy Javanmard, Shaghayegh Sharifi, Mohamadreza |
author_sort | Noori, Jahangir |
collection | PubMed |
description | OBJECTIVE(S): Growing evidences have indicated microRNAs as modulators of tumor development and aggression. On the other hand, a phenomenon known as epithelial-mesenchymal transition (EMT) that indicates a transient phase from epithelial-like features to mesenchymal phenotype is a key player in tumor progression. In this study, we aimed to assess the potential impacts of miR-30a-5p as an inhibitor of melanoma progression and metastasis. MATERIALS AND METHODS: MiR-30a-5p was transfected into B16-F10 melanoma cells. Then, the B16-F10 cells were injected subcutaneously or intravenously (IV) in to C57BL/6 mice. Then, the mice were euthanized and tumor size, tumor weight, snail1 protein expression and nodules in the lungs were evaluated. RESULTS: The migration of cancerous cells was significantly suppressed in vitro following the ectopic presentation of miR-30a-5p into B16-F10 melanoma cells. Furthermore, the metastatic behavior of the neoplastic cells was further suppressed in a xenograft mouse model of melanoma as observed with limited lung infiltration. We also found that transfected miR-30a-5p into melanoma cells could decrease snail1 and N-cadherin expression. CONCLUSION: MiR-30a-5p may represent an effective therapeutic target for the management of melanoma and other snail-overexpressing neoplasms. |
format | Online Article Text |
id | pubmed-6556508 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-65565082019-06-19 The role of microRNA-30a and downstream snail1 on the growth and metastasis of melanoma tumor Noori, Jahangir Haghjooy Javanmard, Shaghayegh Sharifi, Mohamadreza Iran J Basic Med Sci Original Article OBJECTIVE(S): Growing evidences have indicated microRNAs as modulators of tumor development and aggression. On the other hand, a phenomenon known as epithelial-mesenchymal transition (EMT) that indicates a transient phase from epithelial-like features to mesenchymal phenotype is a key player in tumor progression. In this study, we aimed to assess the potential impacts of miR-30a-5p as an inhibitor of melanoma progression and metastasis. MATERIALS AND METHODS: MiR-30a-5p was transfected into B16-F10 melanoma cells. Then, the B16-F10 cells were injected subcutaneously or intravenously (IV) in to C57BL/6 mice. Then, the mice were euthanized and tumor size, tumor weight, snail1 protein expression and nodules in the lungs were evaluated. RESULTS: The migration of cancerous cells was significantly suppressed in vitro following the ectopic presentation of miR-30a-5p into B16-F10 melanoma cells. Furthermore, the metastatic behavior of the neoplastic cells was further suppressed in a xenograft mouse model of melanoma as observed with limited lung infiltration. We also found that transfected miR-30a-5p into melanoma cells could decrease snail1 and N-cadherin expression. CONCLUSION: MiR-30a-5p may represent an effective therapeutic target for the management of melanoma and other snail-overexpressing neoplasms. Mashhad University of Medical Sciences 2019-05 /pmc/articles/PMC6556508/ /pubmed/31217934 http://dx.doi.org/10.22038/IJBMS.2019.32317.7745 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Noori, Jahangir Haghjooy Javanmard, Shaghayegh Sharifi, Mohamadreza The role of microRNA-30a and downstream snail1 on the growth and metastasis of melanoma tumor |
title | The role of microRNA-30a and downstream snail1 on the growth and metastasis of melanoma tumor |
title_full | The role of microRNA-30a and downstream snail1 on the growth and metastasis of melanoma tumor |
title_fullStr | The role of microRNA-30a and downstream snail1 on the growth and metastasis of melanoma tumor |
title_full_unstemmed | The role of microRNA-30a and downstream snail1 on the growth and metastasis of melanoma tumor |
title_short | The role of microRNA-30a and downstream snail1 on the growth and metastasis of melanoma tumor |
title_sort | role of microrna-30a and downstream snail1 on the growth and metastasis of melanoma tumor |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6556508/ https://www.ncbi.nlm.nih.gov/pubmed/31217934 http://dx.doi.org/10.22038/IJBMS.2019.32317.7745 |
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