Decreased expression of MUC2 due to a decrease in the expression of lectins and apoptotic defects in colitis patients

INTRODUCTION: The involvement of mucin, lectin, and apoptosis in colitis is still unclear. This study aimed to investigate changes in MUC2 expression, inflammation, and changes in lectin expression in colitis patients. METHODS: A total of 17 patients were divided into two groups including 11 hemorrh...

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Autores principales: Wibowo, Agung A., Pardjianto, Bambang, Sumitro, Sutiman B., Kania, Nia, Handono, Kusworini
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6556545/
https://www.ncbi.nlm.nih.gov/pubmed/31198858
http://dx.doi.org/10.1016/j.bbrep.2019.100655
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author Wibowo, Agung A.
Pardjianto, Bambang
Sumitro, Sutiman B.
Kania, Nia
Handono, Kusworini
author_facet Wibowo, Agung A.
Pardjianto, Bambang
Sumitro, Sutiman B.
Kania, Nia
Handono, Kusworini
author_sort Wibowo, Agung A.
collection PubMed
description INTRODUCTION: The involvement of mucin, lectin, and apoptosis in colitis is still unclear. This study aimed to investigate changes in MUC2 expression, inflammation, and changes in lectin expression in colitis patients. METHODS: A total of 17 patients were divided into two groups including 11 hemorrhoid patients as a control group and 6 colitis patients. MUC2 mutation analysis was carried out using immunofluorescent and FISH techniques. Assessment of caspase-3, Ki-67, NF-kB, and lectin expressions was also carried out by immunofluorescent technique then analyzed by confocal laser scanning microscope. RESULTS: The MUC2, caspase-3, and lectin expressions were significantly lower in the colitis group than in the control group (p < 0.05). CONCLUSIONS: It was concluded that in colitis there was a change in MUC2 expression due to changes in lectins accompanied by apoptotic defects.conclusion
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spelling pubmed-65565452019-06-13 Decreased expression of MUC2 due to a decrease in the expression of lectins and apoptotic defects in colitis patients Wibowo, Agung A. Pardjianto, Bambang Sumitro, Sutiman B. Kania, Nia Handono, Kusworini Biochem Biophys Rep Research Article INTRODUCTION: The involvement of mucin, lectin, and apoptosis in colitis is still unclear. This study aimed to investigate changes in MUC2 expression, inflammation, and changes in lectin expression in colitis patients. METHODS: A total of 17 patients were divided into two groups including 11 hemorrhoid patients as a control group and 6 colitis patients. MUC2 mutation analysis was carried out using immunofluorescent and FISH techniques. Assessment of caspase-3, Ki-67, NF-kB, and lectin expressions was also carried out by immunofluorescent technique then analyzed by confocal laser scanning microscope. RESULTS: The MUC2, caspase-3, and lectin expressions were significantly lower in the colitis group than in the control group (p < 0.05). CONCLUSIONS: It was concluded that in colitis there was a change in MUC2 expression due to changes in lectins accompanied by apoptotic defects.conclusion Elsevier 2019-06-05 /pmc/articles/PMC6556545/ /pubmed/31198858 http://dx.doi.org/10.1016/j.bbrep.2019.100655 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Article
Wibowo, Agung A.
Pardjianto, Bambang
Sumitro, Sutiman B.
Kania, Nia
Handono, Kusworini
Decreased expression of MUC2 due to a decrease in the expression of lectins and apoptotic defects in colitis patients
title Decreased expression of MUC2 due to a decrease in the expression of lectins and apoptotic defects in colitis patients
title_full Decreased expression of MUC2 due to a decrease in the expression of lectins and apoptotic defects in colitis patients
title_fullStr Decreased expression of MUC2 due to a decrease in the expression of lectins and apoptotic defects in colitis patients
title_full_unstemmed Decreased expression of MUC2 due to a decrease in the expression of lectins and apoptotic defects in colitis patients
title_short Decreased expression of MUC2 due to a decrease in the expression of lectins and apoptotic defects in colitis patients
title_sort decreased expression of muc2 due to a decrease in the expression of lectins and apoptotic defects in colitis patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6556545/
https://www.ncbi.nlm.nih.gov/pubmed/31198858
http://dx.doi.org/10.1016/j.bbrep.2019.100655
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