Metatranscriptome of human fecal microbial communities in a cohort of adult men

The gut microbiome is intimately related to human health, but it is not yet known which functional activities are driven by specific microbes’ ecological configurations or transcription. We report a large-scale investigation of 372 human fecal metatranscriptomes and 929 metagenomes from a subset of 308 men in the Health Professionals Follow-up Study. We identified a metatranscriptomic “core” universally transcribed over time and across participants, often by different microbes. In contrast to the housekeeping functions enriched in this core, a “variable” metatranscriptome included specialized pathways that were differentially expressed both across participants and among microbes. Finally, longitudinal metagenomic profiles allowed ecological interaction network reconstruction, which remained stable over the six-month timespan, as did strain tracking within and between participants. These results provide an initial characterization of human fecal microbial ecology into core, subject-specific, microbe-specific, and temporally-variable transcription, and they differentiate metagenomically versus metatranscriptomically informative aspects of the human fecal microbiome.