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Functional interplay between YY1 and CARM1 promotes oral carcinogenesis
Coactivator associated arginine methyltransferase 1 (CARM1) has been functionally implicated in maintenance of pluripotency, cellular differentiation and tumorigenesis; where it plays regulatory roles by virtue of its ability to coactivate transcription as well as to modulate protein function as an...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6557205/ https://www.ncbi.nlm.nih.gov/pubmed/31217904 http://dx.doi.org/10.18632/oncotarget.26984 |
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author | Behera, Amit K. Kumar, Manoj Shanmugam, Muthu K. Bhattacharya, Aditya Rao, Vinay J. Bhat, Akshay Vasudevan, Madavan Gopinath, Kodaganur S. Mohiyuddin, Azeem Chatterjee, Anupam Sethi, Gautam Kundu, Tapas K. |
author_facet | Behera, Amit K. Kumar, Manoj Shanmugam, Muthu K. Bhattacharya, Aditya Rao, Vinay J. Bhat, Akshay Vasudevan, Madavan Gopinath, Kodaganur S. Mohiyuddin, Azeem Chatterjee, Anupam Sethi, Gautam Kundu, Tapas K. |
author_sort | Behera, Amit K. |
collection | PubMed |
description | Coactivator associated arginine methyltransferase 1 (CARM1) has been functionally implicated in maintenance of pluripotency, cellular differentiation and tumorigenesis; where it plays regulatory roles by virtue of its ability to coactivate transcription as well as to modulate protein function as an arginine methyltransferase. Previous studies establish an oncogenic function of CARM1 in the context of colorectal and breast cancer, which correlate to its overexpressed condition. However, the mechanism behind its deregulated expression in the context of cancer has not been addressed before. In the present study we uncover an oncogenic function of CARM1 in the context of oral cancer, where it was found to be overexpressed. We also identify YY1 to be a positive regulator of CARM1 gene promoter, where silencing of YY1 in oral cancer cell line could lead to reduction in expression of CARM1. In this context, YY1 showed concomitant overexpression in oral cancer patient samples compared to adjacent normal tissue. Cell line based experiments as well as xenograft study revealed pro-neoplastic functions of YY1 in oral cancer. Transcriptomics analysis as well as qRT-PCR validation clearly indicated pro-proliferative, pro-angiogenic and pro-metastatic role of YY1 in oral cancer. We also show that YY1 is a substrate of CARM1 mediated arginine methylation, where the latter could coactivate YY1 mediated reporter gene activation in vivo. Taken together, CARM1 and YY1 were found to regulate each other in a positive feedback loop to facilitate oral cancer progression. |
format | Online Article Text |
id | pubmed-6557205 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-65572052019-06-19 Functional interplay between YY1 and CARM1 promotes oral carcinogenesis Behera, Amit K. Kumar, Manoj Shanmugam, Muthu K. Bhattacharya, Aditya Rao, Vinay J. Bhat, Akshay Vasudevan, Madavan Gopinath, Kodaganur S. Mohiyuddin, Azeem Chatterjee, Anupam Sethi, Gautam Kundu, Tapas K. Oncotarget Research Paper Coactivator associated arginine methyltransferase 1 (CARM1) has been functionally implicated in maintenance of pluripotency, cellular differentiation and tumorigenesis; where it plays regulatory roles by virtue of its ability to coactivate transcription as well as to modulate protein function as an arginine methyltransferase. Previous studies establish an oncogenic function of CARM1 in the context of colorectal and breast cancer, which correlate to its overexpressed condition. However, the mechanism behind its deregulated expression in the context of cancer has not been addressed before. In the present study we uncover an oncogenic function of CARM1 in the context of oral cancer, where it was found to be overexpressed. We also identify YY1 to be a positive regulator of CARM1 gene promoter, where silencing of YY1 in oral cancer cell line could lead to reduction in expression of CARM1. In this context, YY1 showed concomitant overexpression in oral cancer patient samples compared to adjacent normal tissue. Cell line based experiments as well as xenograft study revealed pro-neoplastic functions of YY1 in oral cancer. Transcriptomics analysis as well as qRT-PCR validation clearly indicated pro-proliferative, pro-angiogenic and pro-metastatic role of YY1 in oral cancer. We also show that YY1 is a substrate of CARM1 mediated arginine methylation, where the latter could coactivate YY1 mediated reporter gene activation in vivo. Taken together, CARM1 and YY1 were found to regulate each other in a positive feedback loop to facilitate oral cancer progression. Impact Journals LLC 2019-06-04 /pmc/articles/PMC6557205/ /pubmed/31217904 http://dx.doi.org/10.18632/oncotarget.26984 Text en Copyright: © 2019 Behera et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Behera, Amit K. Kumar, Manoj Shanmugam, Muthu K. Bhattacharya, Aditya Rao, Vinay J. Bhat, Akshay Vasudevan, Madavan Gopinath, Kodaganur S. Mohiyuddin, Azeem Chatterjee, Anupam Sethi, Gautam Kundu, Tapas K. Functional interplay between YY1 and CARM1 promotes oral carcinogenesis |
title | Functional interplay between YY1 and CARM1 promotes oral carcinogenesis |
title_full | Functional interplay between YY1 and CARM1 promotes oral carcinogenesis |
title_fullStr | Functional interplay between YY1 and CARM1 promotes oral carcinogenesis |
title_full_unstemmed | Functional interplay between YY1 and CARM1 promotes oral carcinogenesis |
title_short | Functional interplay between YY1 and CARM1 promotes oral carcinogenesis |
title_sort | functional interplay between yy1 and carm1 promotes oral carcinogenesis |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6557205/ https://www.ncbi.nlm.nih.gov/pubmed/31217904 http://dx.doi.org/10.18632/oncotarget.26984 |
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