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Endothelial Cells Filopodia in the Anastomosis of Central Nervous System Capillaries

In this article we explore filopodia of endothelial cells (ECs) in the developing central nervous system (CNS) using the Golgi method and transmission electron microscopy. Filopodia of ECs play a crucial role in the anastomosis of growing capillaries of the CNS. The leading ECs filopodia from approa...

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Autores principales: Marín-Padilla, Miguel, Howard, Louisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6557217/
https://www.ncbi.nlm.nih.gov/pubmed/31213992
http://dx.doi.org/10.3389/fnana.2019.00049
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author Marín-Padilla, Miguel
Howard, Louisa
author_facet Marín-Padilla, Miguel
Howard, Louisa
author_sort Marín-Padilla, Miguel
collection PubMed
description In this article we explore filopodia of endothelial cells (ECs) in the developing central nervous system (CNS) using the Golgi method and transmission electron microscopy. Filopodia of ECs play a crucial role in the anastomosis of growing capillaries of the CNS. The leading ECs filopodia from approaching capillaries interconnect forming complex conglomerates that precede the anastomotic event. The contacting filopodia form narrow spaces between them filled with proteinaceous basal lamina material. The original narrow spaces coalesce into larger ones leading to the formation of a single one that will interconnect (anastomose) the two approaching capillaries. The four leading ECs (two for each approaching capillary) become the wall of the newly formed post-anastomotic CNS capillaries. These new CNS capillaries are very small with narrow and irregular lumina that might permit the passage of fluid but not yet of blood cells. Eventually, their lumen enlarges and permits the passage of blood cells.
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spelling pubmed-65572172019-06-18 Endothelial Cells Filopodia in the Anastomosis of Central Nervous System Capillaries Marín-Padilla, Miguel Howard, Louisa Front Neuroanat Neuroscience In this article we explore filopodia of endothelial cells (ECs) in the developing central nervous system (CNS) using the Golgi method and transmission electron microscopy. Filopodia of ECs play a crucial role in the anastomosis of growing capillaries of the CNS. The leading ECs filopodia from approaching capillaries interconnect forming complex conglomerates that precede the anastomotic event. The contacting filopodia form narrow spaces between them filled with proteinaceous basal lamina material. The original narrow spaces coalesce into larger ones leading to the formation of a single one that will interconnect (anastomose) the two approaching capillaries. The four leading ECs (two for each approaching capillary) become the wall of the newly formed post-anastomotic CNS capillaries. These new CNS capillaries are very small with narrow and irregular lumina that might permit the passage of fluid but not yet of blood cells. Eventually, their lumen enlarges and permits the passage of blood cells. Frontiers Media S.A. 2019-06-03 /pmc/articles/PMC6557217/ /pubmed/31213992 http://dx.doi.org/10.3389/fnana.2019.00049 Text en Copyright © 2019 Marín-Padilla and Howard. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Marín-Padilla, Miguel
Howard, Louisa
Endothelial Cells Filopodia in the Anastomosis of Central Nervous System Capillaries
title Endothelial Cells Filopodia in the Anastomosis of Central Nervous System Capillaries
title_full Endothelial Cells Filopodia in the Anastomosis of Central Nervous System Capillaries
title_fullStr Endothelial Cells Filopodia in the Anastomosis of Central Nervous System Capillaries
title_full_unstemmed Endothelial Cells Filopodia in the Anastomosis of Central Nervous System Capillaries
title_short Endothelial Cells Filopodia in the Anastomosis of Central Nervous System Capillaries
title_sort endothelial cells filopodia in the anastomosis of central nervous system capillaries
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6557217/
https://www.ncbi.nlm.nih.gov/pubmed/31213992
http://dx.doi.org/10.3389/fnana.2019.00049
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