Epi-illumination SPIM for Volumetric Imaging with High Spatial-temporal Resolution

We designed an epi-illumination SPIM system which utilizes a single objective and has an identical sample interface as an inverted fluorescence microscope with no additional reflection elements. It achieves subcellular resolution and single-molecule sensitivity and is compatible with common biologic...

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Detalles Bibliográficos
Autores principales: Yang, Bin, Chen, Xingye, Wang, Yina, Feng, Siyu, Pessino, Veronica, Stuurman, Nico, Cho, Nathan H., Cheng, Karen W., Lord, Samuel J., Xu, Linfeng, Xie, Dan, Mullins, R. Dyche, Leonetti, Manuel D., Huang, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6557432/
https://www.ncbi.nlm.nih.gov/pubmed/31061492
http://dx.doi.org/10.1038/s41592-019-0401-3
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author Yang, Bin
Chen, Xingye
Wang, Yina
Feng, Siyu
Pessino, Veronica
Stuurman, Nico
Cho, Nathan H.
Cheng, Karen W.
Lord, Samuel J.
Xu, Linfeng
Xie, Dan
Mullins, R. Dyche
Leonetti, Manuel D.
Huang, Bo
author_facet Yang, Bin
Chen, Xingye
Wang, Yina
Feng, Siyu
Pessino, Veronica
Stuurman, Nico
Cho, Nathan H.
Cheng, Karen W.
Lord, Samuel J.
Xu, Linfeng
Xie, Dan
Mullins, R. Dyche
Leonetti, Manuel D.
Huang, Bo
author_sort Yang, Bin
collection PubMed
description We designed an epi-illumination SPIM system which utilizes a single objective and has an identical sample interface as an inverted fluorescence microscope with no additional reflection elements. It achieves subcellular resolution and single-molecule sensitivity and is compatible with common biological sample holders, including multi-well plates. We demonstrated multicolor fast volumetric imaging, single-molecule localization microscopy, parallel imaging of sixteen cell lines and parallel recording of cellular responses to perturbations.
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spelling pubmed-65574322019-11-06 Epi-illumination SPIM for Volumetric Imaging with High Spatial-temporal Resolution Yang, Bin Chen, Xingye Wang, Yina Feng, Siyu Pessino, Veronica Stuurman, Nico Cho, Nathan H. Cheng, Karen W. Lord, Samuel J. Xu, Linfeng Xie, Dan Mullins, R. Dyche Leonetti, Manuel D. Huang, Bo Nat Methods Article We designed an epi-illumination SPIM system which utilizes a single objective and has an identical sample interface as an inverted fluorescence microscope with no additional reflection elements. It achieves subcellular resolution and single-molecule sensitivity and is compatible with common biological sample holders, including multi-well plates. We demonstrated multicolor fast volumetric imaging, single-molecule localization microscopy, parallel imaging of sixteen cell lines and parallel recording of cellular responses to perturbations. 2019-05-06 2019-06 /pmc/articles/PMC6557432/ /pubmed/31061492 http://dx.doi.org/10.1038/s41592-019-0401-3 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Yang, Bin
Chen, Xingye
Wang, Yina
Feng, Siyu
Pessino, Veronica
Stuurman, Nico
Cho, Nathan H.
Cheng, Karen W.
Lord, Samuel J.
Xu, Linfeng
Xie, Dan
Mullins, R. Dyche
Leonetti, Manuel D.
Huang, Bo
Epi-illumination SPIM for Volumetric Imaging with High Spatial-temporal Resolution
title Epi-illumination SPIM for Volumetric Imaging with High Spatial-temporal Resolution
title_full Epi-illumination SPIM for Volumetric Imaging with High Spatial-temporal Resolution
title_fullStr Epi-illumination SPIM for Volumetric Imaging with High Spatial-temporal Resolution
title_full_unstemmed Epi-illumination SPIM for Volumetric Imaging with High Spatial-temporal Resolution
title_short Epi-illumination SPIM for Volumetric Imaging with High Spatial-temporal Resolution
title_sort epi-illumination spim for volumetric imaging with high spatial-temporal resolution
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6557432/
https://www.ncbi.nlm.nih.gov/pubmed/31061492
http://dx.doi.org/10.1038/s41592-019-0401-3
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