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Circulating levels of inflammatory markers and DNA methylation, an analysis of repeated samples from a population based cohort

DNA methylation in blood may adapt to conditions affecting our health, such as inflammation, and multiple studies have identified differential DNA methylation related to smoking, obesity and various diseases. The purpose of this study was to evaluate previously reported, and explore possible new, as...

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Autores principales: Myte, Robin, Sundkvist, Anneli, Van Guelpen, Bethany, Harlid, Sophia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6557598/
https://www.ncbi.nlm.nih.gov/pubmed/31033411
http://dx.doi.org/10.1080/15592294.2019.1603962
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author Myte, Robin
Sundkvist, Anneli
Van Guelpen, Bethany
Harlid, Sophia
author_facet Myte, Robin
Sundkvist, Anneli
Van Guelpen, Bethany
Harlid, Sophia
author_sort Myte, Robin
collection PubMed
description DNA methylation in blood may adapt to conditions affecting our health, such as inflammation, and multiple studies have identified differential DNA methylation related to smoking, obesity and various diseases. The purpose of this study was to evaluate previously reported, and explore possible new, associations between levels of inflammatory markers and DNA methylation in blood. We used a well-characterized study population consisting of 127 individuals, all of whom were participants in the population-based Västerbotten Intervention Programme cohort and had provided two blood samples, ten years apart. Levels of CRP and 160 other proteins were measured in plasma, and DNA methylation levels (assessed using the 850K Illumina Infinium MethylationEPIC BeadChip) were measured in white blood cell DNA. Associations between CpG methylation and protein levels were estimated using linear mixed models. In the study we were able to confirm the direction for 85 of 102 previously reported protein-methylation associations. Depicting associations in a network allowed us to identify CpG sites with associations to multiple proteins, and ten CpG sites were each associated with three or more inflammatory markers. Furthermore, two genetic regions included nine additional unreported CpG sites that may represent trans-acting methylation sites. Our study supports a complex interaction between DNA methylation and circulating proteins involved in the inflammatory response. The notion of trans-acting methylation sites affecting, or being affected by, the expression of genes on completely different chromosomes should be taken into account when interpreting results from epigenome-wide association studies.
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spelling pubmed-65575982019-06-19 Circulating levels of inflammatory markers and DNA methylation, an analysis of repeated samples from a population based cohort Myte, Robin Sundkvist, Anneli Van Guelpen, Bethany Harlid, Sophia Epigenetics Research Paper DNA methylation in blood may adapt to conditions affecting our health, such as inflammation, and multiple studies have identified differential DNA methylation related to smoking, obesity and various diseases. The purpose of this study was to evaluate previously reported, and explore possible new, associations between levels of inflammatory markers and DNA methylation in blood. We used a well-characterized study population consisting of 127 individuals, all of whom were participants in the population-based Västerbotten Intervention Programme cohort and had provided two blood samples, ten years apart. Levels of CRP and 160 other proteins were measured in plasma, and DNA methylation levels (assessed using the 850K Illumina Infinium MethylationEPIC BeadChip) were measured in white blood cell DNA. Associations between CpG methylation and protein levels were estimated using linear mixed models. In the study we were able to confirm the direction for 85 of 102 previously reported protein-methylation associations. Depicting associations in a network allowed us to identify CpG sites with associations to multiple proteins, and ten CpG sites were each associated with three or more inflammatory markers. Furthermore, two genetic regions included nine additional unreported CpG sites that may represent trans-acting methylation sites. Our study supports a complex interaction between DNA methylation and circulating proteins involved in the inflammatory response. The notion of trans-acting methylation sites affecting, or being affected by, the expression of genes on completely different chromosomes should be taken into account when interpreting results from epigenome-wide association studies. Taylor & Francis 2019-04-29 /pmc/articles/PMC6557598/ /pubmed/31033411 http://dx.doi.org/10.1080/15592294.2019.1603962 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Myte, Robin
Sundkvist, Anneli
Van Guelpen, Bethany
Harlid, Sophia
Circulating levels of inflammatory markers and DNA methylation, an analysis of repeated samples from a population based cohort
title Circulating levels of inflammatory markers and DNA methylation, an analysis of repeated samples from a population based cohort
title_full Circulating levels of inflammatory markers and DNA methylation, an analysis of repeated samples from a population based cohort
title_fullStr Circulating levels of inflammatory markers and DNA methylation, an analysis of repeated samples from a population based cohort
title_full_unstemmed Circulating levels of inflammatory markers and DNA methylation, an analysis of repeated samples from a population based cohort
title_short Circulating levels of inflammatory markers and DNA methylation, an analysis of repeated samples from a population based cohort
title_sort circulating levels of inflammatory markers and dna methylation, an analysis of repeated samples from a population based cohort
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6557598/
https://www.ncbi.nlm.nih.gov/pubmed/31033411
http://dx.doi.org/10.1080/15592294.2019.1603962
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