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CBLL1 is highly expressed in non‐small cell lung cancer and promotes cell proliferation and invasion

BACKGROUND: Studies have shown that E3 ubiquitin ligase CBLL1 plays multiple roles in development and tumorigenesis. CBLL1 is over‐expressed in colon cancer and associated with cancer cell proliferation. While, the overexpression of CBLL1 inhibited the estrogenic dependent cell proliferation and mig...

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Autores principales: Hui, Linping, Zhang, Siyang, Wudu, Muli, Ren, Hongjiu, Xu, Yitong, Zhang, Qingfu, Qiu, Xueshan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons Australia, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6558451/
https://www.ncbi.nlm.nih.gov/pubmed/31124298
http://dx.doi.org/10.1111/1759-7714.13097
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author Hui, Linping
Zhang, Siyang
Wudu, Muli
Ren, Hongjiu
Xu, Yitong
Zhang, Qingfu
Qiu, Xueshan
author_facet Hui, Linping
Zhang, Siyang
Wudu, Muli
Ren, Hongjiu
Xu, Yitong
Zhang, Qingfu
Qiu, Xueshan
author_sort Hui, Linping
collection PubMed
description BACKGROUND: Studies have shown that E3 ubiquitin ligase CBLL1 plays multiple roles in development and tumorigenesis. CBLL1 is over‐expressed in colon cancer and associated with cancer cell proliferation. While, the overexpression of CBLL1 inhibited the estrogenic dependent cell proliferation and migration in ER alpha dependent breast cancer cell MCF‐7. METHODS: We used an immunohistochemical method to detect CBLL1 expression in human NSCLC and corresponding normal lung tissues and analyzed its relationship with clinicopathological parameters. Moreover, we investigated the role of CBLL1 in NSCLC cell behavior by inhibiting its expression in A549 and H1299 cells. RESULTS: In this study, we found that CBLL1 was frequently upregulated in non‐small lung cancer (NSCLC) tissues compared to the adjacent nontumor tissues. We found that the high expression of CBLL1 was associated with the tumor size in NSCLC tissues. It has been recently reported that CBLL1 promotes cell proliferation and invasion in A549 and H460 cells. Our results confirmed that CBLL1 promoted the proliferation by promoting G1/S cell cycle transition in NSCLCs cells. Moreover, CBLL1 knockdown inhibited cell invasion via increased E‐cadherin protein expression, and decreased expression of MMP2 and MMP9 in NSCLC cell lines. The protein expression of E‐cadherin was increased after CBLL1 depletion while the E‐cadherin mRNA was not affected after knockdown of the endogenous CBLL1. CONCLUSION: These results provide important insights for using CBLL1 as an oncogenic marker gene in the development and progression of non‐small cell lung cancer.
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spelling pubmed-65584512019-06-13 CBLL1 is highly expressed in non‐small cell lung cancer and promotes cell proliferation and invasion Hui, Linping Zhang, Siyang Wudu, Muli Ren, Hongjiu Xu, Yitong Zhang, Qingfu Qiu, Xueshan Thorac Cancer Original Articles BACKGROUND: Studies have shown that E3 ubiquitin ligase CBLL1 plays multiple roles in development and tumorigenesis. CBLL1 is over‐expressed in colon cancer and associated with cancer cell proliferation. While, the overexpression of CBLL1 inhibited the estrogenic dependent cell proliferation and migration in ER alpha dependent breast cancer cell MCF‐7. METHODS: We used an immunohistochemical method to detect CBLL1 expression in human NSCLC and corresponding normal lung tissues and analyzed its relationship with clinicopathological parameters. Moreover, we investigated the role of CBLL1 in NSCLC cell behavior by inhibiting its expression in A549 and H1299 cells. RESULTS: In this study, we found that CBLL1 was frequently upregulated in non‐small lung cancer (NSCLC) tissues compared to the adjacent nontumor tissues. We found that the high expression of CBLL1 was associated with the tumor size in NSCLC tissues. It has been recently reported that CBLL1 promotes cell proliferation and invasion in A549 and H460 cells. Our results confirmed that CBLL1 promoted the proliferation by promoting G1/S cell cycle transition in NSCLCs cells. Moreover, CBLL1 knockdown inhibited cell invasion via increased E‐cadherin protein expression, and decreased expression of MMP2 and MMP9 in NSCLC cell lines. The protein expression of E‐cadherin was increased after CBLL1 depletion while the E‐cadherin mRNA was not affected after knockdown of the endogenous CBLL1. CONCLUSION: These results provide important insights for using CBLL1 as an oncogenic marker gene in the development and progression of non‐small cell lung cancer. John Wiley & Sons Australia, Ltd 2019-05-23 2019-06 /pmc/articles/PMC6558451/ /pubmed/31124298 http://dx.doi.org/10.1111/1759-7714.13097 Text en © 2019 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Hui, Linping
Zhang, Siyang
Wudu, Muli
Ren, Hongjiu
Xu, Yitong
Zhang, Qingfu
Qiu, Xueshan
CBLL1 is highly expressed in non‐small cell lung cancer and promotes cell proliferation and invasion
title CBLL1 is highly expressed in non‐small cell lung cancer and promotes cell proliferation and invasion
title_full CBLL1 is highly expressed in non‐small cell lung cancer and promotes cell proliferation and invasion
title_fullStr CBLL1 is highly expressed in non‐small cell lung cancer and promotes cell proliferation and invasion
title_full_unstemmed CBLL1 is highly expressed in non‐small cell lung cancer and promotes cell proliferation and invasion
title_short CBLL1 is highly expressed in non‐small cell lung cancer and promotes cell proliferation and invasion
title_sort cbll1 is highly expressed in non‐small cell lung cancer and promotes cell proliferation and invasion
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6558451/
https://www.ncbi.nlm.nih.gov/pubmed/31124298
http://dx.doi.org/10.1111/1759-7714.13097
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