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Exploring the tissue tropism of pseudorabies virus based on miRNA level analysis
BACKGROUND: Pseudorabies virus (PRV, or suid herpesvirus, SuHV-1), a member of the herpesvirus family, has an extremely broad host range and threatens the pig industry in China. PRV can evade host innate immunity and infect the kidney, lung, brain and other tissues. At the same time, many studies ha...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6558711/ https://www.ncbi.nlm.nih.gov/pubmed/31185898 http://dx.doi.org/10.1186/s12866-019-1497-4 |
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author | Fan, Yi Zhu, Ling Sun, Xiangang Lyu, Wenting Xu, Lei Yin, Yue Zhao, Jun Huang, Jianbo Den, Yichao Jiang, Zhiyi Xu, Shiyao Mao, Xiyu Xu, Zhiwen |
author_facet | Fan, Yi Zhu, Ling Sun, Xiangang Lyu, Wenting Xu, Lei Yin, Yue Zhao, Jun Huang, Jianbo Den, Yichao Jiang, Zhiyi Xu, Shiyao Mao, Xiyu Xu, Zhiwen |
author_sort | Fan, Yi |
collection | PubMed |
description | BACKGROUND: Pseudorabies virus (PRV, or suid herpesvirus, SuHV-1), a member of the herpesvirus family, has an extremely broad host range and threatens the pig industry in China. PRV can evade host innate immunity and infect the kidney, lung, brain and other tissues. At the same time, many studies have reported that microRNA (miRNA) can affect the replication of viruses by regulating gene expression levels. RESULTS: Here, to identify changes in miRNA expression and post-transcriptional regulation associated with PRV infection in the lung, spleen, and olfactory bulb, we sequenced small RNAs in tissues of rats infected or uninfected with PRV strain XJ (PRV-XJ). Sixty-one, 199 and 29 differentially-expressed miRNAs were identified in the lung, spleen, and olfactory bulb, respectively, of infected compared with uninfected rats. Among the miRNAs differentially-expressed in PRV-infected rats, 36, 171, and 15 miRNAs showed tissue-selective expression in the olfactory bulb, lung and spleen, respectively. All differentially-expressed miRNAs were analyzed for their GO functional annotations and KEGG pathway associations . CONCLUSIONS: In PRV-XJ-infected rats, miRNAs were differentially expressed in the lung, spleen and olfactory bulb. These miRNAs were involved in regulating various pathways of the nervous, respiratory and immune systems, and may affect the tissue tropism of the virus and play pivotal roles in viral infection and proliferation. |
format | Online Article Text |
id | pubmed-6558711 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-65587112019-06-13 Exploring the tissue tropism of pseudorabies virus based on miRNA level analysis Fan, Yi Zhu, Ling Sun, Xiangang Lyu, Wenting Xu, Lei Yin, Yue Zhao, Jun Huang, Jianbo Den, Yichao Jiang, Zhiyi Xu, Shiyao Mao, Xiyu Xu, Zhiwen BMC Microbiol Research Article BACKGROUND: Pseudorabies virus (PRV, or suid herpesvirus, SuHV-1), a member of the herpesvirus family, has an extremely broad host range and threatens the pig industry in China. PRV can evade host innate immunity and infect the kidney, lung, brain and other tissues. At the same time, many studies have reported that microRNA (miRNA) can affect the replication of viruses by regulating gene expression levels. RESULTS: Here, to identify changes in miRNA expression and post-transcriptional regulation associated with PRV infection in the lung, spleen, and olfactory bulb, we sequenced small RNAs in tissues of rats infected or uninfected with PRV strain XJ (PRV-XJ). Sixty-one, 199 and 29 differentially-expressed miRNAs were identified in the lung, spleen, and olfactory bulb, respectively, of infected compared with uninfected rats. Among the miRNAs differentially-expressed in PRV-infected rats, 36, 171, and 15 miRNAs showed tissue-selective expression in the olfactory bulb, lung and spleen, respectively. All differentially-expressed miRNAs were analyzed for their GO functional annotations and KEGG pathway associations . CONCLUSIONS: In PRV-XJ-infected rats, miRNAs were differentially expressed in the lung, spleen and olfactory bulb. These miRNAs were involved in regulating various pathways of the nervous, respiratory and immune systems, and may affect the tissue tropism of the virus and play pivotal roles in viral infection and proliferation. BioMed Central 2019-06-11 /pmc/articles/PMC6558711/ /pubmed/31185898 http://dx.doi.org/10.1186/s12866-019-1497-4 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Fan, Yi Zhu, Ling Sun, Xiangang Lyu, Wenting Xu, Lei Yin, Yue Zhao, Jun Huang, Jianbo Den, Yichao Jiang, Zhiyi Xu, Shiyao Mao, Xiyu Xu, Zhiwen Exploring the tissue tropism of pseudorabies virus based on miRNA level analysis |
title | Exploring the tissue tropism of pseudorabies virus based on miRNA level analysis |
title_full | Exploring the tissue tropism of pseudorabies virus based on miRNA level analysis |
title_fullStr | Exploring the tissue tropism of pseudorabies virus based on miRNA level analysis |
title_full_unstemmed | Exploring the tissue tropism of pseudorabies virus based on miRNA level analysis |
title_short | Exploring the tissue tropism of pseudorabies virus based on miRNA level analysis |
title_sort | exploring the tissue tropism of pseudorabies virus based on mirna level analysis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6558711/ https://www.ncbi.nlm.nih.gov/pubmed/31185898 http://dx.doi.org/10.1186/s12866-019-1497-4 |
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