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miR-6716-5p promotes metastasis of colorectal cancer through downregulating NAT10 expression
Background: Human N-acetyltransferase 10 (NAT10) plays pivotal roles in cellular biological processes, such as senescence, autophagy and cytokinesis. The expression of NAT10 is dysregulated in colorectal cancer (CRC) and is associated with the prognosis of patients. However, it remains unclear how N...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6559146/ https://www.ncbi.nlm.nih.gov/pubmed/31239781 http://dx.doi.org/10.2147/CMAR.S197733 |
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author | Liu, Zhenzhen Liu, Xiaofeng Li, Yuan Ren, Pengwei Zhang, Chunfeng Wang, Lijun Du, Xiaojuan Xing, Baocai |
author_facet | Liu, Zhenzhen Liu, Xiaofeng Li, Yuan Ren, Pengwei Zhang, Chunfeng Wang, Lijun Du, Xiaojuan Xing, Baocai |
author_sort | Liu, Zhenzhen |
collection | PubMed |
description | Background: Human N-acetyltransferase 10 (NAT10) plays pivotal roles in cellular biological processes, such as senescence, autophagy and cytokinesis. The expression of NAT10 is dysregulated in colorectal cancer (CRC) and is associated with the prognosis of patients. However, it remains unclear how NAT10 is regulated in CRC. Methods: The microRNA(miRNA) regulating NAT10 was predicted by bioinformatics analysis and further validated by real-time quantitative PCR(RT-qPCR),Western blot and dual luciferase reporter assays. The expression of the miRNA regulating NAT10 in CRC tissues was examined using RT-qPCR. Cell proliferation, cell apoptosis, cell migration and cell invasion assays were performed after transfection with miRNA mimic and inhibitor. Results: Here, we report that miR-6716-5p inhibits the expression of NAT10 in CRC. The NAT10 protein level was downregulated by the miR-6716-5p mimic, and was upregulated by the miR-6716-5p inhibitor in CRC cell lines. In addition, miR-6716-5p bound to the 3ʹ-untranslated region of NAT10 mRNA and decreased NAT10 mRNA levels. Significantly, the miR-6716-5p level was higher in the tumor tissues of the CRC patients with liver metastasis than that in the non-metastatic CRC patients. In addition, the miR-6716-5p level was correlated with poor overall survival of CRC patients with liver metastasis. The miR-6716-5p inhibitor inhibited CRC cell migration and invasion. Consistently, the miR-6716-5p mimic significantly promoted cell migration and invasion, and this effect is dependent on NAT10. However, miR-6716-5p had no effect on CRC cell proliferation and apoptosis. We found that miR-6716-5p negatively regulated E-cadherin protein levels. In addition, E-cadherin was upregulated by NAT10 in CRC cells, confirming that miR-6716-5p downregulated E-cadherin levels by inhibiting NAT10 expression. Conclusion: We demonstrated that miR-6716-5p acts as a crucial regulator of NAT10 to promote cell migration and invasion in CRC by inhibiting NAT10 expression. Our data suggest that miR-6716-5p/NAT10 might act as a potential therapeutic target for CRC treatment. |
format | Online Article Text |
id | pubmed-6559146 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-65591462019-06-25 miR-6716-5p promotes metastasis of colorectal cancer through downregulating NAT10 expression Liu, Zhenzhen Liu, Xiaofeng Li, Yuan Ren, Pengwei Zhang, Chunfeng Wang, Lijun Du, Xiaojuan Xing, Baocai Cancer Manag Res Original Research Background: Human N-acetyltransferase 10 (NAT10) plays pivotal roles in cellular biological processes, such as senescence, autophagy and cytokinesis. The expression of NAT10 is dysregulated in colorectal cancer (CRC) and is associated with the prognosis of patients. However, it remains unclear how NAT10 is regulated in CRC. Methods: The microRNA(miRNA) regulating NAT10 was predicted by bioinformatics analysis and further validated by real-time quantitative PCR(RT-qPCR),Western blot and dual luciferase reporter assays. The expression of the miRNA regulating NAT10 in CRC tissues was examined using RT-qPCR. Cell proliferation, cell apoptosis, cell migration and cell invasion assays were performed after transfection with miRNA mimic and inhibitor. Results: Here, we report that miR-6716-5p inhibits the expression of NAT10 in CRC. The NAT10 protein level was downregulated by the miR-6716-5p mimic, and was upregulated by the miR-6716-5p inhibitor in CRC cell lines. In addition, miR-6716-5p bound to the 3ʹ-untranslated region of NAT10 mRNA and decreased NAT10 mRNA levels. Significantly, the miR-6716-5p level was higher in the tumor tissues of the CRC patients with liver metastasis than that in the non-metastatic CRC patients. In addition, the miR-6716-5p level was correlated with poor overall survival of CRC patients with liver metastasis. The miR-6716-5p inhibitor inhibited CRC cell migration and invasion. Consistently, the miR-6716-5p mimic significantly promoted cell migration and invasion, and this effect is dependent on NAT10. However, miR-6716-5p had no effect on CRC cell proliferation and apoptosis. We found that miR-6716-5p negatively regulated E-cadherin protein levels. In addition, E-cadherin was upregulated by NAT10 in CRC cells, confirming that miR-6716-5p downregulated E-cadherin levels by inhibiting NAT10 expression. Conclusion: We demonstrated that miR-6716-5p acts as a crucial regulator of NAT10 to promote cell migration and invasion in CRC by inhibiting NAT10 expression. Our data suggest that miR-6716-5p/NAT10 might act as a potential therapeutic target for CRC treatment. Dove 2019-06-06 /pmc/articles/PMC6559146/ /pubmed/31239781 http://dx.doi.org/10.2147/CMAR.S197733 Text en © 2019 Liu et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Liu, Zhenzhen Liu, Xiaofeng Li, Yuan Ren, Pengwei Zhang, Chunfeng Wang, Lijun Du, Xiaojuan Xing, Baocai miR-6716-5p promotes metastasis of colorectal cancer through downregulating NAT10 expression |
title | miR-6716-5p promotes metastasis of colorectal cancer through downregulating NAT10 expression |
title_full | miR-6716-5p promotes metastasis of colorectal cancer through downregulating NAT10 expression |
title_fullStr | miR-6716-5p promotes metastasis of colorectal cancer through downregulating NAT10 expression |
title_full_unstemmed | miR-6716-5p promotes metastasis of colorectal cancer through downregulating NAT10 expression |
title_short | miR-6716-5p promotes metastasis of colorectal cancer through downregulating NAT10 expression |
title_sort | mir-6716-5p promotes metastasis of colorectal cancer through downregulating nat10 expression |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6559146/ https://www.ncbi.nlm.nih.gov/pubmed/31239781 http://dx.doi.org/10.2147/CMAR.S197733 |
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