Cargando…
Genome sequence and characterization of the bcs clusters for the production of nanocellulose from the low pH resistant strain Komagataeibacter medellinensis ID13488
Komagataeibacter medellinensis ID13488 (formerly Gluconacetobacter medellinensis ID13488) is able to produce crystalline bacterial cellulose (BC) under high acidic growth conditions. These abilities make this strain desirable for industrial BC production from acidic residues (e.g. wastes generated f...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6559206/ https://www.ncbi.nlm.nih.gov/pubmed/30793484 http://dx.doi.org/10.1111/1751-7915.13376 |
_version_ | 1783425790720868352 |
---|---|
author | Hernández‐Arriaga, Ana M. del Cerro, Carlos Urbina, Leire Eceiza, Arantxa Corcuera, Mª Angeles Retegi, Aloña Auxiliadora Prieto, M. |
author_facet | Hernández‐Arriaga, Ana M. del Cerro, Carlos Urbina, Leire Eceiza, Arantxa Corcuera, Mª Angeles Retegi, Aloña Auxiliadora Prieto, M. |
author_sort | Hernández‐Arriaga, Ana M. |
collection | PubMed |
description | Komagataeibacter medellinensis ID13488 (formerly Gluconacetobacter medellinensis ID13488) is able to produce crystalline bacterial cellulose (BC) under high acidic growth conditions. These abilities make this strain desirable for industrial BC production from acidic residues (e.g. wastes generated from cider production). To explore the molecular bases of the BC biosynthesis in this bacterium, the genome has been sequenced revealing a sequence of 3.4 Mb containing three putative plasmids of 38.1 kb (pKM01), 4.3 kb (pKM02) and 3.3 Kb (pKM03). Genome comparison analyses of K. medellinensis ID13488 with other cellulose‐producing related strains resulted in the identification of the bcs genes involved in the cellulose biosynthesis. Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4) was studied by RT‐PCR, and their organization in four operons transcribed as four independent polycistronic mRNAs was determined. qRT‐PCR experiments demonstrated that mostly bcs1 and bcs4 are expressed under BC production conditions, suggesting that these operons direct the synthesis of BC. Genomic differences with the close related strain K. medellinensis NBRC 3288 unable to produce BC were also described and discussed. |
format | Online Article Text |
id | pubmed-6559206 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-65592062019-06-13 Genome sequence and characterization of the bcs clusters for the production of nanocellulose from the low pH resistant strain Komagataeibacter medellinensis ID13488 Hernández‐Arriaga, Ana M. del Cerro, Carlos Urbina, Leire Eceiza, Arantxa Corcuera, Mª Angeles Retegi, Aloña Auxiliadora Prieto, M. Microb Biotechnol Research Articles Komagataeibacter medellinensis ID13488 (formerly Gluconacetobacter medellinensis ID13488) is able to produce crystalline bacterial cellulose (BC) under high acidic growth conditions. These abilities make this strain desirable for industrial BC production from acidic residues (e.g. wastes generated from cider production). To explore the molecular bases of the BC biosynthesis in this bacterium, the genome has been sequenced revealing a sequence of 3.4 Mb containing three putative plasmids of 38.1 kb (pKM01), 4.3 kb (pKM02) and 3.3 Kb (pKM03). Genome comparison analyses of K. medellinensis ID13488 with other cellulose‐producing related strains resulted in the identification of the bcs genes involved in the cellulose biosynthesis. Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4) was studied by RT‐PCR, and their organization in four operons transcribed as four independent polycistronic mRNAs was determined. qRT‐PCR experiments demonstrated that mostly bcs1 and bcs4 are expressed under BC production conditions, suggesting that these operons direct the synthesis of BC. Genomic differences with the close related strain K. medellinensis NBRC 3288 unable to produce BC were also described and discussed. John Wiley and Sons Inc. 2019-02-22 /pmc/articles/PMC6559206/ /pubmed/30793484 http://dx.doi.org/10.1111/1751-7915.13376 Text en © 2019 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Hernández‐Arriaga, Ana M. del Cerro, Carlos Urbina, Leire Eceiza, Arantxa Corcuera, Mª Angeles Retegi, Aloña Auxiliadora Prieto, M. Genome sequence and characterization of the bcs clusters for the production of nanocellulose from the low pH resistant strain Komagataeibacter medellinensis ID13488 |
title | Genome sequence and characterization of the bcs clusters for the production of nanocellulose from the low pH resistant strain Komagataeibacter medellinensis
ID13488 |
title_full | Genome sequence and characterization of the bcs clusters for the production of nanocellulose from the low pH resistant strain Komagataeibacter medellinensis
ID13488 |
title_fullStr | Genome sequence and characterization of the bcs clusters for the production of nanocellulose from the low pH resistant strain Komagataeibacter medellinensis
ID13488 |
title_full_unstemmed | Genome sequence and characterization of the bcs clusters for the production of nanocellulose from the low pH resistant strain Komagataeibacter medellinensis
ID13488 |
title_short | Genome sequence and characterization of the bcs clusters for the production of nanocellulose from the low pH resistant strain Komagataeibacter medellinensis
ID13488 |
title_sort | genome sequence and characterization of the bcs clusters for the production of nanocellulose from the low ph resistant strain komagataeibacter medellinensis
id13488 |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6559206/ https://www.ncbi.nlm.nih.gov/pubmed/30793484 http://dx.doi.org/10.1111/1751-7915.13376 |
work_keys_str_mv | AT hernandezarriagaanam genomesequenceandcharacterizationofthebcsclustersfortheproductionofnanocellulosefromthelowphresistantstrainkomagataeibactermedellinensisid13488 AT delcerrocarlos genomesequenceandcharacterizationofthebcsclustersfortheproductionofnanocellulosefromthelowphresistantstrainkomagataeibactermedellinensisid13488 AT urbinaleire genomesequenceandcharacterizationofthebcsclustersfortheproductionofnanocellulosefromthelowphresistantstrainkomagataeibactermedellinensisid13488 AT eceizaarantxa genomesequenceandcharacterizationofthebcsclustersfortheproductionofnanocellulosefromthelowphresistantstrainkomagataeibactermedellinensisid13488 AT corcueramaangeles genomesequenceandcharacterizationofthebcsclustersfortheproductionofnanocellulosefromthelowphresistantstrainkomagataeibactermedellinensisid13488 AT retegialona genomesequenceandcharacterizationofthebcsclustersfortheproductionofnanocellulosefromthelowphresistantstrainkomagataeibactermedellinensisid13488 AT auxiliadoraprietom genomesequenceandcharacterizationofthebcsclustersfortheproductionofnanocellulosefromthelowphresistantstrainkomagataeibactermedellinensisid13488 |