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Live-Attenuated Respiratory Syncytial Virus Vaccine With Deletion of RNA Synthesis Regulatory Protein M2-2 and Cold Passage Mutations Is Overattenuated
BACKGROUND: The live respiratory syncytial virus (RSV) candidate vaccine LIDcpΔM2-2 is attenuated through deletion of M2-2 and 5 cold-passage mutations. METHODS: RSV-seronegative children aged 6–24 months received a single intranasal dose of 10(5) plaque-forming units (PFU) of LIDcpΔM2-2 or placebo....
| Autores principales: | , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6559275/ https://www.ncbi.nlm.nih.gov/pubmed/31211158 http://dx.doi.org/10.1093/ofid/ofz212 |
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| author | Cunningham, Coleen K Karron, Ruth Muresan, Petronella McFarland, Elizabeth J Perlowski, Charlotte Libous, Jennifer Thumar, Bhagvanji Gnanashanmugam, Devasena Moye, Jack Schappell, Elizabeth Barr, Emily Rexroad, Vivian Aziz, Mariam Deville, Jaime Rutstein, Richard Yang, Lijuan Luongo, Cindy Collins, Peter Buchholz, Ursula |
| author_facet | Cunningham, Coleen K Karron, Ruth Muresan, Petronella McFarland, Elizabeth J Perlowski, Charlotte Libous, Jennifer Thumar, Bhagvanji Gnanashanmugam, Devasena Moye, Jack Schappell, Elizabeth Barr, Emily Rexroad, Vivian Aziz, Mariam Deville, Jaime Rutstein, Richard Yang, Lijuan Luongo, Cindy Collins, Peter Buchholz, Ursula |
| author_sort | Cunningham, Coleen K |
| collection | PubMed |
| description | BACKGROUND: The live respiratory syncytial virus (RSV) candidate vaccine LIDcpΔM2-2 is attenuated through deletion of M2-2 and 5 cold-passage mutations. METHODS: RSV-seronegative children aged 6–24 months received a single intranasal dose of 10(5) plaque-forming units (PFU) of LIDcpΔM2-2 or placebo. RSV serum antibodies, vaccine infectivity, and reactogenicity were assessed. RESULTS: Four of 11 (36%) vaccinees shed vaccine virus with median peak titers of 1.6 log(10) PFU/mL by quantitative culture and 4.5 log(10) copies/mL by polymerase chain reaction; 45% had ≥4-fold rise in serum-neutralizing antibodies. Respiratory symptoms or fever were common in vaccinees (64%) and placebo recipients (6/6, 100%). CONCLUSIONS: RSV LIDcpΔM2-2 is overattenuated. Clinical Trial Numbers. NCT02890381, NCT02948127. |
| format | Online Article Text |
| id | pubmed-6559275 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2019 |
| publisher | Oxford University Press |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-65592752019-06-17 Live-Attenuated Respiratory Syncytial Virus Vaccine With Deletion of RNA Synthesis Regulatory Protein M2-2 and Cold Passage Mutations Is Overattenuated Cunningham, Coleen K Karron, Ruth Muresan, Petronella McFarland, Elizabeth J Perlowski, Charlotte Libous, Jennifer Thumar, Bhagvanji Gnanashanmugam, Devasena Moye, Jack Schappell, Elizabeth Barr, Emily Rexroad, Vivian Aziz, Mariam Deville, Jaime Rutstein, Richard Yang, Lijuan Luongo, Cindy Collins, Peter Buchholz, Ursula Open Forum Infect Dis Brief Report BACKGROUND: The live respiratory syncytial virus (RSV) candidate vaccine LIDcpΔM2-2 is attenuated through deletion of M2-2 and 5 cold-passage mutations. METHODS: RSV-seronegative children aged 6–24 months received a single intranasal dose of 10(5) plaque-forming units (PFU) of LIDcpΔM2-2 or placebo. RSV serum antibodies, vaccine infectivity, and reactogenicity were assessed. RESULTS: Four of 11 (36%) vaccinees shed vaccine virus with median peak titers of 1.6 log(10) PFU/mL by quantitative culture and 4.5 log(10) copies/mL by polymerase chain reaction; 45% had ≥4-fold rise in serum-neutralizing antibodies. Respiratory symptoms or fever were common in vaccinees (64%) and placebo recipients (6/6, 100%). CONCLUSIONS: RSV LIDcpΔM2-2 is overattenuated. Clinical Trial Numbers. NCT02890381, NCT02948127. Oxford University Press 2019-05-06 /pmc/articles/PMC6559275/ /pubmed/31211158 http://dx.doi.org/10.1093/ofid/ofz212 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Infectious Diseases Society of America. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
| spellingShingle | Brief Report Cunningham, Coleen K Karron, Ruth Muresan, Petronella McFarland, Elizabeth J Perlowski, Charlotte Libous, Jennifer Thumar, Bhagvanji Gnanashanmugam, Devasena Moye, Jack Schappell, Elizabeth Barr, Emily Rexroad, Vivian Aziz, Mariam Deville, Jaime Rutstein, Richard Yang, Lijuan Luongo, Cindy Collins, Peter Buchholz, Ursula Live-Attenuated Respiratory Syncytial Virus Vaccine With Deletion of RNA Synthesis Regulatory Protein M2-2 and Cold Passage Mutations Is Overattenuated |
| title | Live-Attenuated Respiratory Syncytial Virus Vaccine With Deletion of RNA Synthesis Regulatory Protein M2-2 and Cold Passage Mutations Is Overattenuated |
| title_full | Live-Attenuated Respiratory Syncytial Virus Vaccine With Deletion of RNA Synthesis Regulatory Protein M2-2 and Cold Passage Mutations Is Overattenuated |
| title_fullStr | Live-Attenuated Respiratory Syncytial Virus Vaccine With Deletion of RNA Synthesis Regulatory Protein M2-2 and Cold Passage Mutations Is Overattenuated |
| title_full_unstemmed | Live-Attenuated Respiratory Syncytial Virus Vaccine With Deletion of RNA Synthesis Regulatory Protein M2-2 and Cold Passage Mutations Is Overattenuated |
| title_short | Live-Attenuated Respiratory Syncytial Virus Vaccine With Deletion of RNA Synthesis Regulatory Protein M2-2 and Cold Passage Mutations Is Overattenuated |
| title_sort | live-attenuated respiratory syncytial virus vaccine with deletion of rna synthesis regulatory protein m2-2 and cold passage mutations is overattenuated |
| topic | Brief Report |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6559275/ https://www.ncbi.nlm.nih.gov/pubmed/31211158 http://dx.doi.org/10.1093/ofid/ofz212 |
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