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NLRP3 inflammasome and lipid metabolism analysis based on UPLC-Q-TOF-MS in gouty nephropathy
To determine the differences in plasma metabolism between healthy patients and patients with hyperuricaemia and gouty nephropathy, the present study identified differentially expressed metabolites associated with gouty nephropathy. Furthermore, the NLRP3 inflammasome signalling pathway in gouty neph...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6559305/ https://www.ncbi.nlm.nih.gov/pubmed/31059009 http://dx.doi.org/10.3892/ijmm.2019.4176 |
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author | Zhang, Yan-Zi Sui, Xiao-Lu Xu, Yun-Peng Gu, Feng-Juan Zhang, Ai-Sha Chen, Ji-Hong |
author_facet | Zhang, Yan-Zi Sui, Xiao-Lu Xu, Yun-Peng Gu, Feng-Juan Zhang, Ai-Sha Chen, Ji-Hong |
author_sort | Zhang, Yan-Zi |
collection | PubMed |
description | To determine the differences in plasma metabolism between healthy patients and patients with hyperuricaemia and gouty nephropathy, the present study identified differentially expressed metabolites associated with gouty nephropathy. Furthermore, the NLRP3 inflammasome signalling pathway in gouty nephropathy was explored, and the mechanism of hyperuricaemia-induced renal damage. Adult male patients examined between July 2016 and June 2017 were selected as the patient cohort for the present study from the Affiliated Bao'an Hospital of Shenzhen, Southern Medical University (Shenzhen, China). These patients were divided into three groups of 30 patients each: Control, hyperuricaemia and gouty nephropathy groups. The expression levels of NLRP3, ASC and caspase-1 mRNA and protein were detected in peripheral blood mononuclear cells, and the plasma levels of IL-1β and IL-18. Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to determine differential levels of metabolites between patients from different groups, in order to identify potential biomarkers. The expression of the NLRP3 inflammasome in peripheral blood mononuclear cells, and the levels of IL-1β and IL-18 in the plasma were increased in the gouty nephropathy group compared with the control and hyperuricaemia groups. In addition, 46 metabolites were identified as potential plasma metabolic biomarkers that were able to distinguish gouty nephropathy from hyperuricaemia. The majority of these metabolites were involved in lipid metabolism, in particular the activity of phospholipase A2 and β-oxidation. These data indicated that lipid metabolism and the NLRP3 inflammasome serve a pivotal role in gouty nephropathy. In addition, the results suggested that lipids may mediate the progression of gouty nephropathy through the activity of phospholipase A2, β-oxidation and activation of the NLRP3 inflammasome. |
format | Online Article Text |
id | pubmed-6559305 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-65593052019-06-12 NLRP3 inflammasome and lipid metabolism analysis based on UPLC-Q-TOF-MS in gouty nephropathy Zhang, Yan-Zi Sui, Xiao-Lu Xu, Yun-Peng Gu, Feng-Juan Zhang, Ai-Sha Chen, Ji-Hong Int J Mol Med Articles To determine the differences in plasma metabolism between healthy patients and patients with hyperuricaemia and gouty nephropathy, the present study identified differentially expressed metabolites associated with gouty nephropathy. Furthermore, the NLRP3 inflammasome signalling pathway in gouty nephropathy was explored, and the mechanism of hyperuricaemia-induced renal damage. Adult male patients examined between July 2016 and June 2017 were selected as the patient cohort for the present study from the Affiliated Bao'an Hospital of Shenzhen, Southern Medical University (Shenzhen, China). These patients were divided into three groups of 30 patients each: Control, hyperuricaemia and gouty nephropathy groups. The expression levels of NLRP3, ASC and caspase-1 mRNA and protein were detected in peripheral blood mononuclear cells, and the plasma levels of IL-1β and IL-18. Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to determine differential levels of metabolites between patients from different groups, in order to identify potential biomarkers. The expression of the NLRP3 inflammasome in peripheral blood mononuclear cells, and the levels of IL-1β and IL-18 in the plasma were increased in the gouty nephropathy group compared with the control and hyperuricaemia groups. In addition, 46 metabolites were identified as potential plasma metabolic biomarkers that were able to distinguish gouty nephropathy from hyperuricaemia. The majority of these metabolites were involved in lipid metabolism, in particular the activity of phospholipase A2 and β-oxidation. These data indicated that lipid metabolism and the NLRP3 inflammasome serve a pivotal role in gouty nephropathy. In addition, the results suggested that lipids may mediate the progression of gouty nephropathy through the activity of phospholipase A2, β-oxidation and activation of the NLRP3 inflammasome. D.A. Spandidos 2019-07 2019-04-30 /pmc/articles/PMC6559305/ /pubmed/31059009 http://dx.doi.org/10.3892/ijmm.2019.4176 Text en Copyright: © Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zhang, Yan-Zi Sui, Xiao-Lu Xu, Yun-Peng Gu, Feng-Juan Zhang, Ai-Sha Chen, Ji-Hong NLRP3 inflammasome and lipid metabolism analysis based on UPLC-Q-TOF-MS in gouty nephropathy |
title | NLRP3 inflammasome and lipid metabolism analysis based on UPLC-Q-TOF-MS in gouty nephropathy |
title_full | NLRP3 inflammasome and lipid metabolism analysis based on UPLC-Q-TOF-MS in gouty nephropathy |
title_fullStr | NLRP3 inflammasome and lipid metabolism analysis based on UPLC-Q-TOF-MS in gouty nephropathy |
title_full_unstemmed | NLRP3 inflammasome and lipid metabolism analysis based on UPLC-Q-TOF-MS in gouty nephropathy |
title_short | NLRP3 inflammasome and lipid metabolism analysis based on UPLC-Q-TOF-MS in gouty nephropathy |
title_sort | nlrp3 inflammasome and lipid metabolism analysis based on uplc-q-tof-ms in gouty nephropathy |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6559305/ https://www.ncbi.nlm.nih.gov/pubmed/31059009 http://dx.doi.org/10.3892/ijmm.2019.4176 |
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