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Proteomics analysis of fetal growth restriction and taurine-treated fetal growth restriction rat brain tissue by 2D DIGE and MALDI-TOF/TOF MS analysis

Fetal growth restriction (FGR) is caused by placental insufficiency and can lead to short and long-term neurodevelopmental delays. Taurine, one of the most abundant amino acids in the brain, is critical for the normal growth and development of the nervous system; however, the mechanistic role of tau...

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Autores principales: Liu, Haifeng, Wang, Yan, Liu, Jing, Fu, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6559329/
https://www.ncbi.nlm.nih.gov/pubmed/31115483
http://dx.doi.org/10.3892/ijmm.2019.4182
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author Liu, Haifeng
Wang, Yan
Liu, Jing
Fu, Wei
author_facet Liu, Haifeng
Wang, Yan
Liu, Jing
Fu, Wei
author_sort Liu, Haifeng
collection PubMed
description Fetal growth restriction (FGR) is caused by placental insufficiency and can lead to short and long-term neurodevelopmental delays. Taurine, one of the most abundant amino acids in the brain, is critical for the normal growth and development of the nervous system; however, the mechanistic role of taurine in neural growth and development remains unknown. The present study investigated the role of taurine in FGR. Specifically, we explored the proteomic profiles of fetal rats at 6 h postpartum by two-dimensional difference gel electrophoresis combined with matrix assisted laser desorption ionization-time-of-flight (TOF)/TOF tandem mass spectrometry; the findings were verified via reverse transcription-quantitative polymerase chain reaction. A total of 31 differentially expressed protein spots were selected. Among these, 31 were matched, including dihydropyrimidinase-related protein 2 and, CRK and peroxiredoxin 2. Functional analysis using the Gene Ontology database and Ingenuity Pathway Analysis demonstrated that the differentially expressed proteins were mainly associated with neuronal differentiation, 'metabolic process', 'biological regulation' and developmental processes. The present study identified several proteins that were differentially expressed in rats with FGR in the presence or absence of taurine administration. The results of the present study suggest a potential role for taurine in the treatment and prevention of FGR.
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spelling pubmed-65593292019-06-12 Proteomics analysis of fetal growth restriction and taurine-treated fetal growth restriction rat brain tissue by 2D DIGE and MALDI-TOF/TOF MS analysis Liu, Haifeng Wang, Yan Liu, Jing Fu, Wei Int J Mol Med Articles Fetal growth restriction (FGR) is caused by placental insufficiency and can lead to short and long-term neurodevelopmental delays. Taurine, one of the most abundant amino acids in the brain, is critical for the normal growth and development of the nervous system; however, the mechanistic role of taurine in neural growth and development remains unknown. The present study investigated the role of taurine in FGR. Specifically, we explored the proteomic profiles of fetal rats at 6 h postpartum by two-dimensional difference gel electrophoresis combined with matrix assisted laser desorption ionization-time-of-flight (TOF)/TOF tandem mass spectrometry; the findings were verified via reverse transcription-quantitative polymerase chain reaction. A total of 31 differentially expressed protein spots were selected. Among these, 31 were matched, including dihydropyrimidinase-related protein 2 and, CRK and peroxiredoxin 2. Functional analysis using the Gene Ontology database and Ingenuity Pathway Analysis demonstrated that the differentially expressed proteins were mainly associated with neuronal differentiation, 'metabolic process', 'biological regulation' and developmental processes. The present study identified several proteins that were differentially expressed in rats with FGR in the presence or absence of taurine administration. The results of the present study suggest a potential role for taurine in the treatment and prevention of FGR. D.A. Spandidos 2019-07 2019-05-07 /pmc/articles/PMC6559329/ /pubmed/31115483 http://dx.doi.org/10.3892/ijmm.2019.4182 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Liu, Haifeng
Wang, Yan
Liu, Jing
Fu, Wei
Proteomics analysis of fetal growth restriction and taurine-treated fetal growth restriction rat brain tissue by 2D DIGE and MALDI-TOF/TOF MS analysis
title Proteomics analysis of fetal growth restriction and taurine-treated fetal growth restriction rat brain tissue by 2D DIGE and MALDI-TOF/TOF MS analysis
title_full Proteomics analysis of fetal growth restriction and taurine-treated fetal growth restriction rat brain tissue by 2D DIGE and MALDI-TOF/TOF MS analysis
title_fullStr Proteomics analysis of fetal growth restriction and taurine-treated fetal growth restriction rat brain tissue by 2D DIGE and MALDI-TOF/TOF MS analysis
title_full_unstemmed Proteomics analysis of fetal growth restriction and taurine-treated fetal growth restriction rat brain tissue by 2D DIGE and MALDI-TOF/TOF MS analysis
title_short Proteomics analysis of fetal growth restriction and taurine-treated fetal growth restriction rat brain tissue by 2D DIGE and MALDI-TOF/TOF MS analysis
title_sort proteomics analysis of fetal growth restriction and taurine-treated fetal growth restriction rat brain tissue by 2d dige and maldi-tof/tof ms analysis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6559329/
https://www.ncbi.nlm.nih.gov/pubmed/31115483
http://dx.doi.org/10.3892/ijmm.2019.4182
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