Cargando…

Effects of BARLEYmax and high-β-glucan barley line on short-chain fatty acids production and microbiota from the cecum to the distal colon in rats

We investigated whether supplementation with the barley line BARLEYmax (Tantangara; BM), which contains three fermentable fibers (fructan, β-glucan, and resistant starch), modifies the microbiota in cecal and distal colonic digesta in addition to short-chain fatty acids (SCFAs) production more favor...

Descripción completa

Detalles Bibliográficos
Autores principales: Aoe, Seiichiro, Yamanaka, Chiemi, Fuwa, Miki, Tamiya, Taiga, Nakayama, Yasunori, Miyoshi, Takanori, Kitazono, Eiichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6559638/
https://www.ncbi.nlm.nih.gov/pubmed/31185060
http://dx.doi.org/10.1371/journal.pone.0218118
_version_ 1783425834532470784
author Aoe, Seiichiro
Yamanaka, Chiemi
Fuwa, Miki
Tamiya, Taiga
Nakayama, Yasunori
Miyoshi, Takanori
Kitazono, Eiichi
author_facet Aoe, Seiichiro
Yamanaka, Chiemi
Fuwa, Miki
Tamiya, Taiga
Nakayama, Yasunori
Miyoshi, Takanori
Kitazono, Eiichi
author_sort Aoe, Seiichiro
collection PubMed
description We investigated whether supplementation with the barley line BARLEYmax (Tantangara; BM), which contains three fermentable fibers (fructan, β-glucan, and resistant starch), modifies the microbiota in cecal and distal colonic digesta in addition to short-chain fatty acids (SCFAs) production more favorably than supplementation with a high-β-glucan barley line (BG012; BG). Male Sprague–Dawley rats were randomly divided into 3 groups that were fed an AIN-93G-based diet that contained 5% fiber provided by cellulose (control), BM or BG. Four weeks after starting the respective diets, the animals were sacrificed and digesta from the cecum, proximal colon and distal colon were collected and the SCFA concentrations were quantified. Microbiota in the cecal and distal colonic digesta were analyzed by 16S rRNA sequencing. The concentrations of acetate and n-butyrate in cecal digesta were significantly higher in the BM and BG groups than in the control group, whereas the concentration of total SCFAs in cecal digesta was significantly higher only in the BM group than in the control group. The concentrations of acetate and total SCFAs in the distal colonic digesta were significantly higher only in the BM group than in the control group. The abundance of Bacteroidetes in cecal digesta was significantly higher in the BM group than in the control group. In contrast, the abundance of Firmicutes in cecal digesta was significantly lower in the BM and BG groups than in the control group. These results indicated that BM increased the concentration of total SCFAs in the distal colonic digesta. These changes might have been caused by fructan and resistant starch in addition to β-glucan. In conclusion, fermentable fibers in BM reached the distal colon and modified the microbiota, leading to an increase in the concentration of total SCFAs in the distal colonic digesta, more effectively compared with the high-β-glucan barley line (BG).
format Online
Article
Text
id pubmed-6559638
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-65596382019-06-17 Effects of BARLEYmax and high-β-glucan barley line on short-chain fatty acids production and microbiota from the cecum to the distal colon in rats Aoe, Seiichiro Yamanaka, Chiemi Fuwa, Miki Tamiya, Taiga Nakayama, Yasunori Miyoshi, Takanori Kitazono, Eiichi PLoS One Research Article We investigated whether supplementation with the barley line BARLEYmax (Tantangara; BM), which contains three fermentable fibers (fructan, β-glucan, and resistant starch), modifies the microbiota in cecal and distal colonic digesta in addition to short-chain fatty acids (SCFAs) production more favorably than supplementation with a high-β-glucan barley line (BG012; BG). Male Sprague–Dawley rats were randomly divided into 3 groups that were fed an AIN-93G-based diet that contained 5% fiber provided by cellulose (control), BM or BG. Four weeks after starting the respective diets, the animals were sacrificed and digesta from the cecum, proximal colon and distal colon were collected and the SCFA concentrations were quantified. Microbiota in the cecal and distal colonic digesta were analyzed by 16S rRNA sequencing. The concentrations of acetate and n-butyrate in cecal digesta were significantly higher in the BM and BG groups than in the control group, whereas the concentration of total SCFAs in cecal digesta was significantly higher only in the BM group than in the control group. The concentrations of acetate and total SCFAs in the distal colonic digesta were significantly higher only in the BM group than in the control group. The abundance of Bacteroidetes in cecal digesta was significantly higher in the BM group than in the control group. In contrast, the abundance of Firmicutes in cecal digesta was significantly lower in the BM and BG groups than in the control group. These results indicated that BM increased the concentration of total SCFAs in the distal colonic digesta. These changes might have been caused by fructan and resistant starch in addition to β-glucan. In conclusion, fermentable fibers in BM reached the distal colon and modified the microbiota, leading to an increase in the concentration of total SCFAs in the distal colonic digesta, more effectively compared with the high-β-glucan barley line (BG). Public Library of Science 2019-06-11 /pmc/articles/PMC6559638/ /pubmed/31185060 http://dx.doi.org/10.1371/journal.pone.0218118 Text en © 2019 Aoe et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Aoe, Seiichiro
Yamanaka, Chiemi
Fuwa, Miki
Tamiya, Taiga
Nakayama, Yasunori
Miyoshi, Takanori
Kitazono, Eiichi
Effects of BARLEYmax and high-β-glucan barley line on short-chain fatty acids production and microbiota from the cecum to the distal colon in rats
title Effects of BARLEYmax and high-β-glucan barley line on short-chain fatty acids production and microbiota from the cecum to the distal colon in rats
title_full Effects of BARLEYmax and high-β-glucan barley line on short-chain fatty acids production and microbiota from the cecum to the distal colon in rats
title_fullStr Effects of BARLEYmax and high-β-glucan barley line on short-chain fatty acids production and microbiota from the cecum to the distal colon in rats
title_full_unstemmed Effects of BARLEYmax and high-β-glucan barley line on short-chain fatty acids production and microbiota from the cecum to the distal colon in rats
title_short Effects of BARLEYmax and high-β-glucan barley line on short-chain fatty acids production and microbiota from the cecum to the distal colon in rats
title_sort effects of barleymax and high-β-glucan barley line on short-chain fatty acids production and microbiota from the cecum to the distal colon in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6559638/
https://www.ncbi.nlm.nih.gov/pubmed/31185060
http://dx.doi.org/10.1371/journal.pone.0218118
work_keys_str_mv AT aoeseiichiro effectsofbarleymaxandhighbglucanbarleylineonshortchainfattyacidsproductionandmicrobiotafromthececumtothedistalcoloninrats
AT yamanakachiemi effectsofbarleymaxandhighbglucanbarleylineonshortchainfattyacidsproductionandmicrobiotafromthececumtothedistalcoloninrats
AT fuwamiki effectsofbarleymaxandhighbglucanbarleylineonshortchainfattyacidsproductionandmicrobiotafromthececumtothedistalcoloninrats
AT tamiyataiga effectsofbarleymaxandhighbglucanbarleylineonshortchainfattyacidsproductionandmicrobiotafromthececumtothedistalcoloninrats
AT nakayamayasunori effectsofbarleymaxandhighbglucanbarleylineonshortchainfattyacidsproductionandmicrobiotafromthececumtothedistalcoloninrats
AT miyoshitakanori effectsofbarleymaxandhighbglucanbarleylineonshortchainfattyacidsproductionandmicrobiotafromthececumtothedistalcoloninrats
AT kitazonoeiichi effectsofbarleymaxandhighbglucanbarleylineonshortchainfattyacidsproductionandmicrobiotafromthececumtothedistalcoloninrats