A novel data processing method CyC* for quantitative real time polymerase chain reaction minimizes cumulative error

Quantitative real-time polymerase chain reaction (qPCR) is routinely conducted for DNA quantitative analysis using the cycle-threshold (Ct) method, which assumes uniform/optimum template amplification. In practice, amplification efficiencies vary from cycle to cycle in a PCR reaction, and often decl...

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Detalles Bibliográficos
Autores principales: Zhang, Linzhong, Dong, Rui, Wei, Shu, Zhou, Han-Chen, Zhang, Meng-Xian, Alagarsamy, Karthikeyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6559663/
https://www.ncbi.nlm.nih.gov/pubmed/31185064
http://dx.doi.org/10.1371/journal.pone.0218159

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6559663/
https://www.ncbi.nlm.nih.gov/pubmed/31185064
http://dx.doi.org/10.1371/journal.pone.0218159

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