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Live cell imaging of meiosis in Arabidopsis thaliana

To follow the dynamics of meiosis in the model plant Arabidopsis, we have established a live cell imaging setup to observe male meiocytes. Our method is based on the concomitant visualization of microtubules (MTs) and a meiotic cohesin subunit that allows following five cellular parameters: cell sha...

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Autores principales: Prusicki, Maria A, Keizer, Emma M, van Rosmalen, Rik P, Komaki, Shinichiro, Seifert, Felix, Müller, Katja, Wijnker, Erik, Fleck, Christian, Schnittger, Arp
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6559805/
https://www.ncbi.nlm.nih.gov/pubmed/31107238
http://dx.doi.org/10.7554/eLife.42834
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author Prusicki, Maria A
Keizer, Emma M
van Rosmalen, Rik P
Komaki, Shinichiro
Seifert, Felix
Müller, Katja
Wijnker, Erik
Fleck, Christian
Schnittger, Arp
author_facet Prusicki, Maria A
Keizer, Emma M
van Rosmalen, Rik P
Komaki, Shinichiro
Seifert, Felix
Müller, Katja
Wijnker, Erik
Fleck, Christian
Schnittger, Arp
author_sort Prusicki, Maria A
collection PubMed
description To follow the dynamics of meiosis in the model plant Arabidopsis, we have established a live cell imaging setup to observe male meiocytes. Our method is based on the concomitant visualization of microtubules (MTs) and a meiotic cohesin subunit that allows following five cellular parameters: cell shape, MT array, nucleus position, nucleolus position, and chromatin condensation. We find that the states of these parameters are not randomly associated and identify 11 cellular states, referred to as landmarks, which occur much more frequently than closely related ones, indicating that they are convergence points during meiotic progression. As a first application of our system, we revisited a previously identified mutant in the meiotic A-type cyclin TARDY ASYNCHRONOUS MEIOSIS (TAM). Our imaging system enabled us to reveal both qualitatively and quantitatively altered landmarks in tam, foremost the formation of previously not recognized ectopic spindle- or phragmoplast-like structures that arise without attachment to chromosomes.
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spelling pubmed-65598052019-06-13 Live cell imaging of meiosis in Arabidopsis thaliana Prusicki, Maria A Keizer, Emma M van Rosmalen, Rik P Komaki, Shinichiro Seifert, Felix Müller, Katja Wijnker, Erik Fleck, Christian Schnittger, Arp eLife Cell Biology To follow the dynamics of meiosis in the model plant Arabidopsis, we have established a live cell imaging setup to observe male meiocytes. Our method is based on the concomitant visualization of microtubules (MTs) and a meiotic cohesin subunit that allows following five cellular parameters: cell shape, MT array, nucleus position, nucleolus position, and chromatin condensation. We find that the states of these parameters are not randomly associated and identify 11 cellular states, referred to as landmarks, which occur much more frequently than closely related ones, indicating that they are convergence points during meiotic progression. As a first application of our system, we revisited a previously identified mutant in the meiotic A-type cyclin TARDY ASYNCHRONOUS MEIOSIS (TAM). Our imaging system enabled us to reveal both qualitatively and quantitatively altered landmarks in tam, foremost the formation of previously not recognized ectopic spindle- or phragmoplast-like structures that arise without attachment to chromosomes. eLife Sciences Publications, Ltd 2019-05-20 /pmc/articles/PMC6559805/ /pubmed/31107238 http://dx.doi.org/10.7554/eLife.42834 Text en © 2019, Prusicki et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Prusicki, Maria A
Keizer, Emma M
van Rosmalen, Rik P
Komaki, Shinichiro
Seifert, Felix
Müller, Katja
Wijnker, Erik
Fleck, Christian
Schnittger, Arp
Live cell imaging of meiosis in Arabidopsis thaliana
title Live cell imaging of meiosis in Arabidopsis thaliana
title_full Live cell imaging of meiosis in Arabidopsis thaliana
title_fullStr Live cell imaging of meiosis in Arabidopsis thaliana
title_full_unstemmed Live cell imaging of meiosis in Arabidopsis thaliana
title_short Live cell imaging of meiosis in Arabidopsis thaliana
title_sort live cell imaging of meiosis in arabidopsis thaliana
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6559805/
https://www.ncbi.nlm.nih.gov/pubmed/31107238
http://dx.doi.org/10.7554/eLife.42834
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