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Amaryllidaceae alkaloids: identification and partial characterization of montanine production in Rhodophiala bifida plant

Rhodophiala bifida (R. bifida) is a representative of the Amaryllidaceae plant family and is rich in montanine, an alkaloid with high pharmaceutical potential. Despite the interest in these compounds, many steps of the biosynthetic pathway have not been elucidated. In this study, we identified the a...

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Autores principales: Reis, Andressa, Magne, Kevin, Massot, Sophie, Tallini, Luciana R., Scopel, Marina, Bastida, Jaume, Ratet, Pascal, Zuanazzi, José A. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6559983/
https://www.ncbi.nlm.nih.gov/pubmed/31186470
http://dx.doi.org/10.1038/s41598-019-44746-7
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author Reis, Andressa
Magne, Kevin
Massot, Sophie
Tallini, Luciana R.
Scopel, Marina
Bastida, Jaume
Ratet, Pascal
Zuanazzi, José A. S.
author_facet Reis, Andressa
Magne, Kevin
Massot, Sophie
Tallini, Luciana R.
Scopel, Marina
Bastida, Jaume
Ratet, Pascal
Zuanazzi, José A. S.
author_sort Reis, Andressa
collection PubMed
description Rhodophiala bifida (R. bifida) is a representative of the Amaryllidaceae plant family and is rich in montanine, an alkaloid with high pharmaceutical potential. Despite the interest in these compounds, many steps of the biosynthetic pathway have not been elucidated. In this study, we identified the alkaloids produced in different organs of R. bifida under different growth conditions, set up the conditions for in vitro R. bifida regeneration and initiated the molecular characterization of two R. bifida genes involved in alkaloids biosynthesis: the Norbelladine 4′-O-Methyltransferase (RbN4OMT) and the Cytochrome P450 (RbCYP96T). We show that montanine is the main alkaloid produced in the different R. bifida organs and developed a direct organogenesis regeneration protocol, using twin-scale explants cultivated on media enriched with naphthalene acetic acid and benzyladenine. Finally, we analyzed the RbN4OMT and RbCYP96T gene expressions in different organs and culture conditions and compared them to alkaloid production. In different organs of R. bifida young, adult and regenerated plants, as well as under various growing conditions, the transcripts accumulation was correlated with the production of alkaloids. This work provides new tools to improve the production of this important pharmaceutical compound and for future biotechnological studies.
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spelling pubmed-65599832019-06-19 Amaryllidaceae alkaloids: identification and partial characterization of montanine production in Rhodophiala bifida plant Reis, Andressa Magne, Kevin Massot, Sophie Tallini, Luciana R. Scopel, Marina Bastida, Jaume Ratet, Pascal Zuanazzi, José A. S. Sci Rep Article Rhodophiala bifida (R. bifida) is a representative of the Amaryllidaceae plant family and is rich in montanine, an alkaloid with high pharmaceutical potential. Despite the interest in these compounds, many steps of the biosynthetic pathway have not been elucidated. In this study, we identified the alkaloids produced in different organs of R. bifida under different growth conditions, set up the conditions for in vitro R. bifida regeneration and initiated the molecular characterization of two R. bifida genes involved in alkaloids biosynthesis: the Norbelladine 4′-O-Methyltransferase (RbN4OMT) and the Cytochrome P450 (RbCYP96T). We show that montanine is the main alkaloid produced in the different R. bifida organs and developed a direct organogenesis regeneration protocol, using twin-scale explants cultivated on media enriched with naphthalene acetic acid and benzyladenine. Finally, we analyzed the RbN4OMT and RbCYP96T gene expressions in different organs and culture conditions and compared them to alkaloid production. In different organs of R. bifida young, adult and regenerated plants, as well as under various growing conditions, the transcripts accumulation was correlated with the production of alkaloids. This work provides new tools to improve the production of this important pharmaceutical compound and for future biotechnological studies. Nature Publishing Group UK 2019-06-11 /pmc/articles/PMC6559983/ /pubmed/31186470 http://dx.doi.org/10.1038/s41598-019-44746-7 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Reis, Andressa
Magne, Kevin
Massot, Sophie
Tallini, Luciana R.
Scopel, Marina
Bastida, Jaume
Ratet, Pascal
Zuanazzi, José A. S.
Amaryllidaceae alkaloids: identification and partial characterization of montanine production in Rhodophiala bifida plant
title Amaryllidaceae alkaloids: identification and partial characterization of montanine production in Rhodophiala bifida plant
title_full Amaryllidaceae alkaloids: identification and partial characterization of montanine production in Rhodophiala bifida plant
title_fullStr Amaryllidaceae alkaloids: identification and partial characterization of montanine production in Rhodophiala bifida plant
title_full_unstemmed Amaryllidaceae alkaloids: identification and partial characterization of montanine production in Rhodophiala bifida plant
title_short Amaryllidaceae alkaloids: identification and partial characterization of montanine production in Rhodophiala bifida plant
title_sort amaryllidaceae alkaloids: identification and partial characterization of montanine production in rhodophiala bifida plant
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6559983/
https://www.ncbi.nlm.nih.gov/pubmed/31186470
http://dx.doi.org/10.1038/s41598-019-44746-7
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