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Evaluation of ultra-low input RNA sequencing for the study of human T cell transcriptome

Deeper understanding of T cell biology is crucial for the development of new therapeutics. Human naïve T cells have low RNA content and their numbers can be limiting; therefore we set out to determine the parameters for robust ultra-low input RNA sequencing. We performed transcriptome profiling at d...

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Detalles Bibliográficos
Autores principales: Wang, Jingya, Rieder, Sadiye Amcaoglu, Wu, Jincheng, Hayes, Susana, Halpin, Rebecca A., de los Reyes, Melissa, Shrestha, Yashaswi, Kolbeck, Roland, Raja, Rajiv
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6559993/
https://www.ncbi.nlm.nih.gov/pubmed/31186477
http://dx.doi.org/10.1038/s41598-019-44902-z