Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection

Mitochondria are key for cellular metabolism and signalling processes during viral infection. We report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging and dete...

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Autores principales: Schneider, Annika, Kurz, Sandra, Manske, Katrin, Janas, Marianne, Heikenwälder, Mathias, Misgeld, Thomas, Aichler, Michaela, Weissmann, Sebastian Felix, Zischka, Hans, Knolle, Percy, Wohlleber, Dirk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6560178/
https://www.ncbi.nlm.nih.gov/pubmed/31186476
http://dx.doi.org/10.1038/s41598-019-44922-9
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author Schneider, Annika
Kurz, Sandra
Manske, Katrin
Janas, Marianne
Heikenwälder, Mathias
Misgeld, Thomas
Aichler, Michaela
Weissmann, Sebastian Felix
Zischka, Hans
Knolle, Percy
Wohlleber, Dirk
author_facet Schneider, Annika
Kurz, Sandra
Manske, Katrin
Janas, Marianne
Heikenwälder, Mathias
Misgeld, Thomas
Aichler, Michaela
Weissmann, Sebastian Felix
Zischka, Hans
Knolle, Percy
Wohlleber, Dirk
author_sort Schneider, Annika
collection PubMed
description Mitochondria are key for cellular metabolism and signalling processes during viral infection. We report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging and detection by multispectral flow cytometry. Resolution at the level of tagged individual mitochondria revealed changes in mitochondrial size, membrane potential and displayed a fragile phenotype during viral infection of cells. Thus, single-organelle and multi-parameter resolution allows to explore altered energy metabolism and antiviral defence by tagged mitochondria selectively in virus-infected cells and will be instrumental to identify viral immune escape and to develop and monitor novel mitochondrial-targeted therapies.
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spelling pubmed-65601782019-06-19 Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection Schneider, Annika Kurz, Sandra Manske, Katrin Janas, Marianne Heikenwälder, Mathias Misgeld, Thomas Aichler, Michaela Weissmann, Sebastian Felix Zischka, Hans Knolle, Percy Wohlleber, Dirk Sci Rep Article Mitochondria are key for cellular metabolism and signalling processes during viral infection. We report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging and detection by multispectral flow cytometry. Resolution at the level of tagged individual mitochondria revealed changes in mitochondrial size, membrane potential and displayed a fragile phenotype during viral infection of cells. Thus, single-organelle and multi-parameter resolution allows to explore altered energy metabolism and antiviral defence by tagged mitochondria selectively in virus-infected cells and will be instrumental to identify viral immune escape and to develop and monitor novel mitochondrial-targeted therapies. Nature Publishing Group UK 2019-06-11 /pmc/articles/PMC6560178/ /pubmed/31186476 http://dx.doi.org/10.1038/s41598-019-44922-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Schneider, Annika
Kurz, Sandra
Manske, Katrin
Janas, Marianne
Heikenwälder, Mathias
Misgeld, Thomas
Aichler, Michaela
Weissmann, Sebastian Felix
Zischka, Hans
Knolle, Percy
Wohlleber, Dirk
Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection
title Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection
title_full Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection
title_fullStr Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection
title_full_unstemmed Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection
title_short Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection
title_sort single organelle analysis to characterize mitochondrial function and crosstalk during viral infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6560178/
https://www.ncbi.nlm.nih.gov/pubmed/31186476
http://dx.doi.org/10.1038/s41598-019-44922-9
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