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Feeding Essential Oils to Neonatal Holstein Dairy Calves Results in Increased Ruminal Prevotellaceae Abundance and Propionate Concentrations

Since antibiotic use in animal production has become a public health concern, great efforts are being dedicated to find effective and viable alternatives. While essential oils (EO) have become attractive candidates for use in the livestock industry, their mode of action and microbial targets in food...

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Autores principales: Poudel, Prakash, Froehlich, Kelly, Casper, David Paul, St-Pierre, Benoit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6560426/
https://www.ncbi.nlm.nih.gov/pubmed/31052458
http://dx.doi.org/10.3390/microorganisms7050120
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author Poudel, Prakash
Froehlich, Kelly
Casper, David Paul
St-Pierre, Benoit
author_facet Poudel, Prakash
Froehlich, Kelly
Casper, David Paul
St-Pierre, Benoit
author_sort Poudel, Prakash
collection PubMed
description Since antibiotic use in animal production has become a public health concern, great efforts are being dedicated to find effective and viable alternatives. While essential oils (EO) have become attractive candidates for use in the livestock industry, their mode of action and microbial targets in food animals remain largely uncharacterized. To gain further insight, we investigated the rumen environment of neonatal calves fed calf starter pellets and milk replacer supplemented with a commercial blend of EO. Propionate concentrations were not only found to be higher in EO-fed calves compared to controls (P < 0.05), but ruminal bacterial communities also differed greatly. For instance, the abundance of Firmicutes was significantly lower in samples from EO-fed calves than in controls, which appeared to be mostly due to lower Lachnospiraceae levels (P < 0.05). In contrast, Bacteriodetes were more abundant in EO-fed calves compared to controls, which was primarily the result of higher Prevotellaceae (P < 0.05). Notably, two bacterial operational taxonomic units (OTUs) were significantly more abundant in EO-fed calves; SD_Bt-00966 was found to be a close relative of Prevotella ruminicola (97%), while SD_Bt-00978 likely corresponded to an uncharacterized species of Gammaproteobacteria. In addition, Pearson correlation and canonical correspondence analyses revealed potential associations between other ruminal bacterial OTUs and either short chain fatty acids (SCFA) parameters or metrics for calf growth. Together, these results support that EO supplementation in growing dairy calves can modulate rumen function through SCFA production and growth of specific rumen bacterial groups.
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spelling pubmed-65604262019-06-17 Feeding Essential Oils to Neonatal Holstein Dairy Calves Results in Increased Ruminal Prevotellaceae Abundance and Propionate Concentrations Poudel, Prakash Froehlich, Kelly Casper, David Paul St-Pierre, Benoit Microorganisms Article Since antibiotic use in animal production has become a public health concern, great efforts are being dedicated to find effective and viable alternatives. While essential oils (EO) have become attractive candidates for use in the livestock industry, their mode of action and microbial targets in food animals remain largely uncharacterized. To gain further insight, we investigated the rumen environment of neonatal calves fed calf starter pellets and milk replacer supplemented with a commercial blend of EO. Propionate concentrations were not only found to be higher in EO-fed calves compared to controls (P < 0.05), but ruminal bacterial communities also differed greatly. For instance, the abundance of Firmicutes was significantly lower in samples from EO-fed calves than in controls, which appeared to be mostly due to lower Lachnospiraceae levels (P < 0.05). In contrast, Bacteriodetes were more abundant in EO-fed calves compared to controls, which was primarily the result of higher Prevotellaceae (P < 0.05). Notably, two bacterial operational taxonomic units (OTUs) were significantly more abundant in EO-fed calves; SD_Bt-00966 was found to be a close relative of Prevotella ruminicola (97%), while SD_Bt-00978 likely corresponded to an uncharacterized species of Gammaproteobacteria. In addition, Pearson correlation and canonical correspondence analyses revealed potential associations between other ruminal bacterial OTUs and either short chain fatty acids (SCFA) parameters or metrics for calf growth. Together, these results support that EO supplementation in growing dairy calves can modulate rumen function through SCFA production and growth of specific rumen bacterial groups. MDPI 2019-05-01 /pmc/articles/PMC6560426/ /pubmed/31052458 http://dx.doi.org/10.3390/microorganisms7050120 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Poudel, Prakash
Froehlich, Kelly
Casper, David Paul
St-Pierre, Benoit
Feeding Essential Oils to Neonatal Holstein Dairy Calves Results in Increased Ruminal Prevotellaceae Abundance and Propionate Concentrations
title Feeding Essential Oils to Neonatal Holstein Dairy Calves Results in Increased Ruminal Prevotellaceae Abundance and Propionate Concentrations
title_full Feeding Essential Oils to Neonatal Holstein Dairy Calves Results in Increased Ruminal Prevotellaceae Abundance and Propionate Concentrations
title_fullStr Feeding Essential Oils to Neonatal Holstein Dairy Calves Results in Increased Ruminal Prevotellaceae Abundance and Propionate Concentrations
title_full_unstemmed Feeding Essential Oils to Neonatal Holstein Dairy Calves Results in Increased Ruminal Prevotellaceae Abundance and Propionate Concentrations
title_short Feeding Essential Oils to Neonatal Holstein Dairy Calves Results in Increased Ruminal Prevotellaceae Abundance and Propionate Concentrations
title_sort feeding essential oils to neonatal holstein dairy calves results in increased ruminal prevotellaceae abundance and propionate concentrations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6560426/
https://www.ncbi.nlm.nih.gov/pubmed/31052458
http://dx.doi.org/10.3390/microorganisms7050120
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