Cargando…

Identification of the Components in a Vaccinium oldhamii Extract Showing Inhibitory Activity against Influenza Virus Adsorption

We previously reported that extracts from plants of the Ericaceae genus Vaccinium, commonly known as the kind of blueberry, inhibited the early steps of influenza virus (IFV) infection to host cells, and that the activity was correlated with the total polyphenol content. Particularly potent inhibito...

Descripción completa

Detalles Bibliográficos
Autores principales: Sekizawa, Haruhito, Ikuta, Kazufumi, Ohnishi-Kameyama, Mayumi, Nishiyama, Kyoko, Suzutani, Tatsuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6560511/
https://www.ncbi.nlm.nih.gov/pubmed/31137514
http://dx.doi.org/10.3390/foods8050172
_version_ 1783425985633320960
author Sekizawa, Haruhito
Ikuta, Kazufumi
Ohnishi-Kameyama, Mayumi
Nishiyama, Kyoko
Suzutani, Tatsuo
author_facet Sekizawa, Haruhito
Ikuta, Kazufumi
Ohnishi-Kameyama, Mayumi
Nishiyama, Kyoko
Suzutani, Tatsuo
author_sort Sekizawa, Haruhito
collection PubMed
description We previously reported that extracts from plants of the Ericaceae genus Vaccinium, commonly known as the kind of blueberry, inhibited the early steps of influenza virus (IFV) infection to host cells, and that the activity was correlated with the total polyphenol content. Particularly potent inhibitory activity was observed for Vaccinium oldhamii. In this study, we identified the active components in Vaccinium oldhamii involved in the inhibition of IFV infection. We sequentially fractionated the Vaccinium oldhamii extract using a synthetic adsorbent resin column. High inhibitory activity was observed for the fractions eluted with 30%, 40%, and 50% ethanol, and three peaks (peak A, B, and C) considered to represent polyphenols were identified in the fractions by HPLC analysis. Among these peaks, high inhibitory activity was detected for peak A and B, but not for peak C. These peaks were analyzed by LC/MS, which revealed that peak A contained procyanidin B2 and ferulic acid derivatives, whereas peak B contained two ferulic acid O-hexosides, and peak C contained quercetin-3-O-rhamnoside and quercetin-O-pentoside-O-rhamnoside. It is already known that these polyphenols have anti-IFV activity, but we speculate that ferulic acid derivatives are the major contributors to the inhibition of the early steps of IFV replication, such as either adsorption or entry, observed for Vaccinium oldhamii.
format Online
Article
Text
id pubmed-6560511
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-65605112019-06-17 Identification of the Components in a Vaccinium oldhamii Extract Showing Inhibitory Activity against Influenza Virus Adsorption Sekizawa, Haruhito Ikuta, Kazufumi Ohnishi-Kameyama, Mayumi Nishiyama, Kyoko Suzutani, Tatsuo Foods Article We previously reported that extracts from plants of the Ericaceae genus Vaccinium, commonly known as the kind of blueberry, inhibited the early steps of influenza virus (IFV) infection to host cells, and that the activity was correlated with the total polyphenol content. Particularly potent inhibitory activity was observed for Vaccinium oldhamii. In this study, we identified the active components in Vaccinium oldhamii involved in the inhibition of IFV infection. We sequentially fractionated the Vaccinium oldhamii extract using a synthetic adsorbent resin column. High inhibitory activity was observed for the fractions eluted with 30%, 40%, and 50% ethanol, and three peaks (peak A, B, and C) considered to represent polyphenols were identified in the fractions by HPLC analysis. Among these peaks, high inhibitory activity was detected for peak A and B, but not for peak C. These peaks were analyzed by LC/MS, which revealed that peak A contained procyanidin B2 and ferulic acid derivatives, whereas peak B contained two ferulic acid O-hexosides, and peak C contained quercetin-3-O-rhamnoside and quercetin-O-pentoside-O-rhamnoside. It is already known that these polyphenols have anti-IFV activity, but we speculate that ferulic acid derivatives are the major contributors to the inhibition of the early steps of IFV replication, such as either adsorption or entry, observed for Vaccinium oldhamii. MDPI 2019-05-20 /pmc/articles/PMC6560511/ /pubmed/31137514 http://dx.doi.org/10.3390/foods8050172 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sekizawa, Haruhito
Ikuta, Kazufumi
Ohnishi-Kameyama, Mayumi
Nishiyama, Kyoko
Suzutani, Tatsuo
Identification of the Components in a Vaccinium oldhamii Extract Showing Inhibitory Activity against Influenza Virus Adsorption
title Identification of the Components in a Vaccinium oldhamii Extract Showing Inhibitory Activity against Influenza Virus Adsorption
title_full Identification of the Components in a Vaccinium oldhamii Extract Showing Inhibitory Activity against Influenza Virus Adsorption
title_fullStr Identification of the Components in a Vaccinium oldhamii Extract Showing Inhibitory Activity against Influenza Virus Adsorption
title_full_unstemmed Identification of the Components in a Vaccinium oldhamii Extract Showing Inhibitory Activity against Influenza Virus Adsorption
title_short Identification of the Components in a Vaccinium oldhamii Extract Showing Inhibitory Activity against Influenza Virus Adsorption
title_sort identification of the components in a vaccinium oldhamii extract showing inhibitory activity against influenza virus adsorption
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6560511/
https://www.ncbi.nlm.nih.gov/pubmed/31137514
http://dx.doi.org/10.3390/foods8050172
work_keys_str_mv AT sekizawaharuhito identificationofthecomponentsinavacciniumoldhamiiextractshowinginhibitoryactivityagainstinfluenzavirusadsorption
AT ikutakazufumi identificationofthecomponentsinavacciniumoldhamiiextractshowinginhibitoryactivityagainstinfluenzavirusadsorption
AT ohnishikameyamamayumi identificationofthecomponentsinavacciniumoldhamiiextractshowinginhibitoryactivityagainstinfluenzavirusadsorption
AT nishiyamakyoko identificationofthecomponentsinavacciniumoldhamiiextractshowinginhibitoryactivityagainstinfluenzavirusadsorption
AT suzutanitatsuo identificationofthecomponentsinavacciniumoldhamiiextractshowinginhibitoryactivityagainstinfluenzavirusadsorption