Cargando…

MicroRNA responses associated with Salmonella enterica serovar typhimurium challenge in peripheral blood: effects of miR-146a and IFN-γ in regulation of fecal bacteria shedding counts in pig

BACKGROUND: MicroRNAs are involved in a broad range of biological processes and are known to be differentially expressed in response to bacterial pathogens. RESULTS: The present study identified microRNA responses in porcine peripheral blood after inoculation with the human foodborne pathogen Salmon...

Descripción completa

Detalles Bibliográficos
Autores principales: Huang, Tinghua, Huang, Xiali, Chen, Wang, Yin, Jun, Shi, Bomei, Wang, Fangfang, Feng, Wenzhao, Yao, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6560770/
https://www.ncbi.nlm.nih.gov/pubmed/31186019
http://dx.doi.org/10.1186/s12917-019-1951-4
_version_ 1783426016854671360
author Huang, Tinghua
Huang, Xiali
Chen, Wang
Yin, Jun
Shi, Bomei
Wang, Fangfang
Feng, Wenzhao
Yao, Min
author_facet Huang, Tinghua
Huang, Xiali
Chen, Wang
Yin, Jun
Shi, Bomei
Wang, Fangfang
Feng, Wenzhao
Yao, Min
author_sort Huang, Tinghua
collection PubMed
description BACKGROUND: MicroRNAs are involved in a broad range of biological processes and are known to be differentially expressed in response to bacterial pathogens. RESULTS: The present study identified microRNA responses in porcine peripheral blood after inoculation with the human foodborne pathogen Salmonella enterica serovar Typhimurium strain LT2. We compared the microRNA transcriptomes of the whole blood of pigs (Duroc × Landrace × Yorkshire) at 2-days post inoculation and before Salmonella infection. The analysis identified a total of 29 differentially expressed microRNAs, most of which are implicated in Salmonella infection and immunology signaling pathways. Joint analysis of the microRNA and mRNA transcriptomes identified 24 microRNAs with binding sites that were significantly enriched in 3′ UTR of differentially expressed mRNAs. Of these microRNAs, three were differentially expressed after Salmonella challenge in peripheral blood (ssc-miR-146a-5p, ssc-miR-125a, and ssc-miR-129a-5p). Expression of 23 targets of top-ranked microRNA, ssc-miR-146a-5p, was validated by real-time PCR. The effects of miR-146a, IFN-γ, and IL-6 on the regulation of fecal bacteria shedding counts in pigs were investigated by in vivo study with a Salmonella challenge model. CONCLUSIONS: The results indicated that induction of miR-146a in peripheral blood could significantly increase the fecal bacterial load, whereas IFN-γ had the reverse effect. These microRNAs can be used to identify targets for controlling porcine salmonellosis.
format Online
Article
Text
id pubmed-6560770
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-65607702019-06-14 MicroRNA responses associated with Salmonella enterica serovar typhimurium challenge in peripheral blood: effects of miR-146a and IFN-γ in regulation of fecal bacteria shedding counts in pig Huang, Tinghua Huang, Xiali Chen, Wang Yin, Jun Shi, Bomei Wang, Fangfang Feng, Wenzhao Yao, Min BMC Vet Res Research Article BACKGROUND: MicroRNAs are involved in a broad range of biological processes and are known to be differentially expressed in response to bacterial pathogens. RESULTS: The present study identified microRNA responses in porcine peripheral blood after inoculation with the human foodborne pathogen Salmonella enterica serovar Typhimurium strain LT2. We compared the microRNA transcriptomes of the whole blood of pigs (Duroc × Landrace × Yorkshire) at 2-days post inoculation and before Salmonella infection. The analysis identified a total of 29 differentially expressed microRNAs, most of which are implicated in Salmonella infection and immunology signaling pathways. Joint analysis of the microRNA and mRNA transcriptomes identified 24 microRNAs with binding sites that were significantly enriched in 3′ UTR of differentially expressed mRNAs. Of these microRNAs, three were differentially expressed after Salmonella challenge in peripheral blood (ssc-miR-146a-5p, ssc-miR-125a, and ssc-miR-129a-5p). Expression of 23 targets of top-ranked microRNA, ssc-miR-146a-5p, was validated by real-time PCR. The effects of miR-146a, IFN-γ, and IL-6 on the regulation of fecal bacteria shedding counts in pigs were investigated by in vivo study with a Salmonella challenge model. CONCLUSIONS: The results indicated that induction of miR-146a in peripheral blood could significantly increase the fecal bacterial load, whereas IFN-γ had the reverse effect. These microRNAs can be used to identify targets for controlling porcine salmonellosis. BioMed Central 2019-06-11 /pmc/articles/PMC6560770/ /pubmed/31186019 http://dx.doi.org/10.1186/s12917-019-1951-4 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Huang, Tinghua
Huang, Xiali
Chen, Wang
Yin, Jun
Shi, Bomei
Wang, Fangfang
Feng, Wenzhao
Yao, Min
MicroRNA responses associated with Salmonella enterica serovar typhimurium challenge in peripheral blood: effects of miR-146a and IFN-γ in regulation of fecal bacteria shedding counts in pig
title MicroRNA responses associated with Salmonella enterica serovar typhimurium challenge in peripheral blood: effects of miR-146a and IFN-γ in regulation of fecal bacteria shedding counts in pig
title_full MicroRNA responses associated with Salmonella enterica serovar typhimurium challenge in peripheral blood: effects of miR-146a and IFN-γ in regulation of fecal bacteria shedding counts in pig
title_fullStr MicroRNA responses associated with Salmonella enterica serovar typhimurium challenge in peripheral blood: effects of miR-146a and IFN-γ in regulation of fecal bacteria shedding counts in pig
title_full_unstemmed MicroRNA responses associated with Salmonella enterica serovar typhimurium challenge in peripheral blood: effects of miR-146a and IFN-γ in regulation of fecal bacteria shedding counts in pig
title_short MicroRNA responses associated with Salmonella enterica serovar typhimurium challenge in peripheral blood: effects of miR-146a and IFN-γ in regulation of fecal bacteria shedding counts in pig
title_sort microrna responses associated with salmonella enterica serovar typhimurium challenge in peripheral blood: effects of mir-146a and ifn-γ in regulation of fecal bacteria shedding counts in pig
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6560770/
https://www.ncbi.nlm.nih.gov/pubmed/31186019
http://dx.doi.org/10.1186/s12917-019-1951-4
work_keys_str_mv AT huangtinghua micrornaresponsesassociatedwithsalmonellaentericaserovartyphimuriumchallengeinperipheralbloodeffectsofmir146aandifnginregulationoffecalbacteriasheddingcountsinpig
AT huangxiali micrornaresponsesassociatedwithsalmonellaentericaserovartyphimuriumchallengeinperipheralbloodeffectsofmir146aandifnginregulationoffecalbacteriasheddingcountsinpig
AT chenwang micrornaresponsesassociatedwithsalmonellaentericaserovartyphimuriumchallengeinperipheralbloodeffectsofmir146aandifnginregulationoffecalbacteriasheddingcountsinpig
AT yinjun micrornaresponsesassociatedwithsalmonellaentericaserovartyphimuriumchallengeinperipheralbloodeffectsofmir146aandifnginregulationoffecalbacteriasheddingcountsinpig
AT shibomei micrornaresponsesassociatedwithsalmonellaentericaserovartyphimuriumchallengeinperipheralbloodeffectsofmir146aandifnginregulationoffecalbacteriasheddingcountsinpig
AT wangfangfang micrornaresponsesassociatedwithsalmonellaentericaserovartyphimuriumchallengeinperipheralbloodeffectsofmir146aandifnginregulationoffecalbacteriasheddingcountsinpig
AT fengwenzhao micrornaresponsesassociatedwithsalmonellaentericaserovartyphimuriumchallengeinperipheralbloodeffectsofmir146aandifnginregulationoffecalbacteriasheddingcountsinpig
AT yaomin micrornaresponsesassociatedwithsalmonellaentericaserovartyphimuriumchallengeinperipheralbloodeffectsofmir146aandifnginregulationoffecalbacteriasheddingcountsinpig