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The Effects of Astragalus Polysaccharide on Bone Marrow-Derived Mesenchymal Stem Cell Proliferation and Morphology Induced by A549 Lung Cancer Cells

BACKGROUND: The tumor microenvironment in lung cancer plays an important role in tumor progression and metastasis. Bone marrow-derived mesenchymal stem cells (MSCs) co-cultured with A549 lung cancer cells show changes in morphology, increase cell proliferation, and cell migration. This study aimed t...

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Autores principales: Zhang, Yue-Mei, Liu, Yong-Qi, Liu, Dongling, Zhang, Liying, Qin, Jie, Zhang, Zhiming, Su, Yun, Yan, Chunlu, Luo, Ya-Li, Li, Jintian, Xie, Xiaodong, Guan, Quanlin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6561146/
https://www.ncbi.nlm.nih.gov/pubmed/31154455
http://dx.doi.org/10.12659/MSM.914219
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author Zhang, Yue-Mei
Liu, Yong-Qi
Liu, Dongling
Zhang, Liying
Qin, Jie
Zhang, Zhiming
Su, Yun
Yan, Chunlu
Luo, Ya-Li
Li, Jintian
Xie, Xiaodong
Guan, Quanlin
author_facet Zhang, Yue-Mei
Liu, Yong-Qi
Liu, Dongling
Zhang, Liying
Qin, Jie
Zhang, Zhiming
Su, Yun
Yan, Chunlu
Luo, Ya-Li
Li, Jintian
Xie, Xiaodong
Guan, Quanlin
author_sort Zhang, Yue-Mei
collection PubMed
description BACKGROUND: The tumor microenvironment in lung cancer plays an important role in tumor progression and metastasis. Bone marrow-derived mesenchymal stem cells (MSCs) co-cultured with A549 lung cancer cells show changes in morphology, increase cell proliferation, and cell migration. This study aimed to investigate the effects of Astragalus polysaccharide (APS), a traditional Chinese herbal medicine, on the changes induced in bone marrow-derived MSCs by A549 lung cancer cells in vitro. MATERIAL/METHODS: Bone marrow-derived MSCs were co-cultured with A549 cells (Co-BMSCs). Co-cultured bone marrow-derived MSCs and A549 cells treated with 50 μg/ml of APS (Co-BMSCs + APS) were compared with untreated Co-BMSCs. Cell proliferation was measured using the cell counting kit-8 (CCK-8) assay. Flow cytometry evaluated the cell cycle. Microarray assays for mRNA expression and Western blot for protein expression were used. RESULTS: Compared with untreated Co-BMSCs, APS treatment of Co-BMSCs improved cell morphology, reduced cell proliferation, and inhibited cell cycle arrest. The mitogen-activated protein kinase (MAPK)/nuclear factor-kappa B (NF-κB) pathway, TP53, caspase-3, acetylated H4K5, acetylated H4K8, and acetylated H3K9 were involved in the regulatory process. CONCLUSIONS: APS treatment reduced cell proliferation and morphological changes in bone marrow-derived MSCs that were co-cultured with A549 lung cancer cells in vitro.
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spelling pubmed-65611462019-06-26 The Effects of Astragalus Polysaccharide on Bone Marrow-Derived Mesenchymal Stem Cell Proliferation and Morphology Induced by A549 Lung Cancer Cells Zhang, Yue-Mei Liu, Yong-Qi Liu, Dongling Zhang, Liying Qin, Jie Zhang, Zhiming Su, Yun Yan, Chunlu Luo, Ya-Li Li, Jintian Xie, Xiaodong Guan, Quanlin Med Sci Monit Lab/In Vitro Research BACKGROUND: The tumor microenvironment in lung cancer plays an important role in tumor progression and metastasis. Bone marrow-derived mesenchymal stem cells (MSCs) co-cultured with A549 lung cancer cells show changes in morphology, increase cell proliferation, and cell migration. This study aimed to investigate the effects of Astragalus polysaccharide (APS), a traditional Chinese herbal medicine, on the changes induced in bone marrow-derived MSCs by A549 lung cancer cells in vitro. MATERIAL/METHODS: Bone marrow-derived MSCs were co-cultured with A549 cells (Co-BMSCs). Co-cultured bone marrow-derived MSCs and A549 cells treated with 50 μg/ml of APS (Co-BMSCs + APS) were compared with untreated Co-BMSCs. Cell proliferation was measured using the cell counting kit-8 (CCK-8) assay. Flow cytometry evaluated the cell cycle. Microarray assays for mRNA expression and Western blot for protein expression were used. RESULTS: Compared with untreated Co-BMSCs, APS treatment of Co-BMSCs improved cell morphology, reduced cell proliferation, and inhibited cell cycle arrest. The mitogen-activated protein kinase (MAPK)/nuclear factor-kappa B (NF-κB) pathway, TP53, caspase-3, acetylated H4K5, acetylated H4K8, and acetylated H3K9 were involved in the regulatory process. CONCLUSIONS: APS treatment reduced cell proliferation and morphological changes in bone marrow-derived MSCs that were co-cultured with A549 lung cancer cells in vitro. International Scientific Literature, Inc. 2019-06-02 /pmc/articles/PMC6561146/ /pubmed/31154455 http://dx.doi.org/10.12659/MSM.914219 Text en © Med Sci Monit, 2019 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) )
spellingShingle Lab/In Vitro Research
Zhang, Yue-Mei
Liu, Yong-Qi
Liu, Dongling
Zhang, Liying
Qin, Jie
Zhang, Zhiming
Su, Yun
Yan, Chunlu
Luo, Ya-Li
Li, Jintian
Xie, Xiaodong
Guan, Quanlin
The Effects of Astragalus Polysaccharide on Bone Marrow-Derived Mesenchymal Stem Cell Proliferation and Morphology Induced by A549 Lung Cancer Cells
title The Effects of Astragalus Polysaccharide on Bone Marrow-Derived Mesenchymal Stem Cell Proliferation and Morphology Induced by A549 Lung Cancer Cells
title_full The Effects of Astragalus Polysaccharide on Bone Marrow-Derived Mesenchymal Stem Cell Proliferation and Morphology Induced by A549 Lung Cancer Cells
title_fullStr The Effects of Astragalus Polysaccharide on Bone Marrow-Derived Mesenchymal Stem Cell Proliferation and Morphology Induced by A549 Lung Cancer Cells
title_full_unstemmed The Effects of Astragalus Polysaccharide on Bone Marrow-Derived Mesenchymal Stem Cell Proliferation and Morphology Induced by A549 Lung Cancer Cells
title_short The Effects of Astragalus Polysaccharide on Bone Marrow-Derived Mesenchymal Stem Cell Proliferation and Morphology Induced by A549 Lung Cancer Cells
title_sort effects of astragalus polysaccharide on bone marrow-derived mesenchymal stem cell proliferation and morphology induced by a549 lung cancer cells
topic Lab/In Vitro Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6561146/
https://www.ncbi.nlm.nih.gov/pubmed/31154455
http://dx.doi.org/10.12659/MSM.914219
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