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CREB-B acts as a key mediator of NPF/NO pathway involved in phase-related locomotor plasticity in locusts

Gene expression changes in neural systems are essential for environment-induced behavioral plasticity in animals; however, neuronal signaling pathways mediating the effect of external stimuli on transcriptional changes are largely unknown. Recently, we have demonstrated that the neuropeptide F (NPF)...

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Detalles Bibliográficos
Autores principales: Hou, Li, Li, Beibei, Ding, Ding, Kang, Le, Wang, Xianhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6561586/
https://www.ncbi.nlm.nih.gov/pubmed/31150381
http://dx.doi.org/10.1371/journal.pgen.1008176
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author Hou, Li
Li, Beibei
Ding, Ding
Kang, Le
Wang, Xianhui
author_facet Hou, Li
Li, Beibei
Ding, Ding
Kang, Le
Wang, Xianhui
author_sort Hou, Li
collection PubMed
description Gene expression changes in neural systems are essential for environment-induced behavioral plasticity in animals; however, neuronal signaling pathways mediating the effect of external stimuli on transcriptional changes are largely unknown. Recently, we have demonstrated that the neuropeptide F (NPF)/nitric oxide (NO) signaling pathway plays a regulatory role in phase-related locomotor plasticity in the migratory locust, Locusta migratoria. Here, we report that a conserved transcription factor, cAMP response element-binding protein B (CREB-B), is a key mediator involved in the signaling pathway from NPF2 to NOS in the migratory locust, triggering locomotor activity shift between solitarious and gregarious phases. We find that CREB-B directly activates brain NOS expression by interacting with NOS promoter region. The phosphorylation at serine 110 site of CREB-B dynamically changes in response to population density variation and is negatively controlled by NPF2. The involvement of CREB-B in NPF2-regulated locomotor plasticity is further validated by RNAi experiment and behavioral assay. Furthermore, we reveal that protein kinase A mediates the regulatory effects of NPF2 on CREB-B phosphorylation and NOS transcription. These findings highlight a precise signal cascade underlying environment-induced behavioral plasticity.
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spelling pubmed-65615862019-06-20 CREB-B acts as a key mediator of NPF/NO pathway involved in phase-related locomotor plasticity in locusts Hou, Li Li, Beibei Ding, Ding Kang, Le Wang, Xianhui PLoS Genet Research Article Gene expression changes in neural systems are essential for environment-induced behavioral plasticity in animals; however, neuronal signaling pathways mediating the effect of external stimuli on transcriptional changes are largely unknown. Recently, we have demonstrated that the neuropeptide F (NPF)/nitric oxide (NO) signaling pathway plays a regulatory role in phase-related locomotor plasticity in the migratory locust, Locusta migratoria. Here, we report that a conserved transcription factor, cAMP response element-binding protein B (CREB-B), is a key mediator involved in the signaling pathway from NPF2 to NOS in the migratory locust, triggering locomotor activity shift between solitarious and gregarious phases. We find that CREB-B directly activates brain NOS expression by interacting with NOS promoter region. The phosphorylation at serine 110 site of CREB-B dynamically changes in response to population density variation and is negatively controlled by NPF2. The involvement of CREB-B in NPF2-regulated locomotor plasticity is further validated by RNAi experiment and behavioral assay. Furthermore, we reveal that protein kinase A mediates the regulatory effects of NPF2 on CREB-B phosphorylation and NOS transcription. These findings highlight a precise signal cascade underlying environment-induced behavioral plasticity. Public Library of Science 2019-05-31 /pmc/articles/PMC6561586/ /pubmed/31150381 http://dx.doi.org/10.1371/journal.pgen.1008176 Text en © 2019 Hou et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Hou, Li
Li, Beibei
Ding, Ding
Kang, Le
Wang, Xianhui
CREB-B acts as a key mediator of NPF/NO pathway involved in phase-related locomotor plasticity in locusts
title CREB-B acts as a key mediator of NPF/NO pathway involved in phase-related locomotor plasticity in locusts
title_full CREB-B acts as a key mediator of NPF/NO pathway involved in phase-related locomotor plasticity in locusts
title_fullStr CREB-B acts as a key mediator of NPF/NO pathway involved in phase-related locomotor plasticity in locusts
title_full_unstemmed CREB-B acts as a key mediator of NPF/NO pathway involved in phase-related locomotor plasticity in locusts
title_short CREB-B acts as a key mediator of NPF/NO pathway involved in phase-related locomotor plasticity in locusts
title_sort creb-b acts as a key mediator of npf/no pathway involved in phase-related locomotor plasticity in locusts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6561586/
https://www.ncbi.nlm.nih.gov/pubmed/31150381
http://dx.doi.org/10.1371/journal.pgen.1008176
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