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Functional Landscape of PCGF Proteins Reveals Both RING1A/B-Dependent-and RING1A/B-Independent-Specific Activities

Polycomb repressive complexes 1 and 2 (PRC1 and PRC2) control cell identity by establishing facultative heterochromatin repressive domains at common sets of target genes. PRC1, which deposits H2Aub1 through the E3 ligases RING1A/B, forms six biochemically distinct subcomplexes depending on the assem...

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Autores principales: Scelfo, Andrea, Fernández-Pérez, Daniel, Tamburri, Simone, Zanotti, Marika, Lavarone, Elisa, Soldi, Monica, Bonaldi, Tiziana, Ferrari, Karin Johanna, Pasini, Diego
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6561742/
https://www.ncbi.nlm.nih.gov/pubmed/31029542
http://dx.doi.org/10.1016/j.molcel.2019.04.002
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author Scelfo, Andrea
Fernández-Pérez, Daniel
Tamburri, Simone
Zanotti, Marika
Lavarone, Elisa
Soldi, Monica
Bonaldi, Tiziana
Ferrari, Karin Johanna
Pasini, Diego
author_facet Scelfo, Andrea
Fernández-Pérez, Daniel
Tamburri, Simone
Zanotti, Marika
Lavarone, Elisa
Soldi, Monica
Bonaldi, Tiziana
Ferrari, Karin Johanna
Pasini, Diego
author_sort Scelfo, Andrea
collection PubMed
description Polycomb repressive complexes 1 and 2 (PRC1 and PRC2) control cell identity by establishing facultative heterochromatin repressive domains at common sets of target genes. PRC1, which deposits H2Aub1 through the E3 ligases RING1A/B, forms six biochemically distinct subcomplexes depending on the assembled PCGF protein (PCGF1–PCGF6); however, it is yet unclear whether these subcomplexes have also specific activities. Here we show that PCGF1 and PCGF2 largely compensate for each other, while other PCGF proteins have high levels of specificity for distinct target genes. PCGF2 associates with transcription repression, whereas PCGF3 and PCGF6 associate with actively transcribed genes. Notably, PCGF3 and PCGF6 complexes can assemble and be recruited to several active sites independently of RING1A/B activity (therefore, of PRC1). For chromatin recruitment, the PCGF6 complex requires the combinatorial activities of its MGA-MAX and E2F6-DP1 subunits, while PCGF3 requires an interaction with the USF1 DNA binding transcription factor.
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spelling pubmed-65617422019-06-17 Functional Landscape of PCGF Proteins Reveals Both RING1A/B-Dependent-and RING1A/B-Independent-Specific Activities Scelfo, Andrea Fernández-Pérez, Daniel Tamburri, Simone Zanotti, Marika Lavarone, Elisa Soldi, Monica Bonaldi, Tiziana Ferrari, Karin Johanna Pasini, Diego Mol Cell Article Polycomb repressive complexes 1 and 2 (PRC1 and PRC2) control cell identity by establishing facultative heterochromatin repressive domains at common sets of target genes. PRC1, which deposits H2Aub1 through the E3 ligases RING1A/B, forms six biochemically distinct subcomplexes depending on the assembled PCGF protein (PCGF1–PCGF6); however, it is yet unclear whether these subcomplexes have also specific activities. Here we show that PCGF1 and PCGF2 largely compensate for each other, while other PCGF proteins have high levels of specificity for distinct target genes. PCGF2 associates with transcription repression, whereas PCGF3 and PCGF6 associate with actively transcribed genes. Notably, PCGF3 and PCGF6 complexes can assemble and be recruited to several active sites independently of RING1A/B activity (therefore, of PRC1). For chromatin recruitment, the PCGF6 complex requires the combinatorial activities of its MGA-MAX and E2F6-DP1 subunits, while PCGF3 requires an interaction with the USF1 DNA binding transcription factor. Cell Press 2019-06-06 /pmc/articles/PMC6561742/ /pubmed/31029542 http://dx.doi.org/10.1016/j.molcel.2019.04.002 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Scelfo, Andrea
Fernández-Pérez, Daniel
Tamburri, Simone
Zanotti, Marika
Lavarone, Elisa
Soldi, Monica
Bonaldi, Tiziana
Ferrari, Karin Johanna
Pasini, Diego
Functional Landscape of PCGF Proteins Reveals Both RING1A/B-Dependent-and RING1A/B-Independent-Specific Activities
title Functional Landscape of PCGF Proteins Reveals Both RING1A/B-Dependent-and RING1A/B-Independent-Specific Activities
title_full Functional Landscape of PCGF Proteins Reveals Both RING1A/B-Dependent-and RING1A/B-Independent-Specific Activities
title_fullStr Functional Landscape of PCGF Proteins Reveals Both RING1A/B-Dependent-and RING1A/B-Independent-Specific Activities
title_full_unstemmed Functional Landscape of PCGF Proteins Reveals Both RING1A/B-Dependent-and RING1A/B-Independent-Specific Activities
title_short Functional Landscape of PCGF Proteins Reveals Both RING1A/B-Dependent-and RING1A/B-Independent-Specific Activities
title_sort functional landscape of pcgf proteins reveals both ring1a/b-dependent-and ring1a/b-independent-specific activities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6561742/
https://www.ncbi.nlm.nih.gov/pubmed/31029542
http://dx.doi.org/10.1016/j.molcel.2019.04.002
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